TaqNovaGC DNA Polymerase is an ideal tool for the amplification of GC-rich templates. A recombinant and thermostable enzyme isolated from Thermus aquaticus, is recommended for a wide range of applications requiring DNA synthesis at extremely high temperatures. The TaqNovaGC DNA polymerase is a universal DNA polymerase which works rapidly and effectively in various PCR conditions. The enzyme catalyses DNA synthesis in a 5’→ 3’ direction, shows no 3’→ 5’ exonuclease activity, but has a 5’→ 3’ exonuclease activity.

The application of appropriate PCR buffer conditions and 5x GC-Additive as the PCR enhancer enables the amplification of specific DNA regions with a high GC-content. This PCR additive changes DNA behavior upon heating and can be used with GC-rich primer-template pairs which do not work well under standard PCR conditions. The 5x GC-Additive reduces the number of secondary structures and enables the specific hybridisation of primers.

Features and advantages

• Recombinant enzyme of high purity
• Extreme yield with minimal amounts of enzyme and little optimization
• Half-life of the enzyme is 45 minutes at 95°C
• Amplifies fragments of up to 5 kb
• High specificity
• Enables the amplification of specific DNA regions with a high GC-content

Applications

• Efficient amplification of short and medium size DNA sequences
• GC-rich templates
• Ideal for problematic templates, which fail with standard Taq DNA polymerases
• Diagnostic PCR
• TA cloning

Unit definition

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.