Available Options:
SKU | Exctractions | ||
---|---|---|---|
![]() | RP85T.1 | 100 rxns | Ask for an offer |
![]() | RP85T-10 | 1000 rxns | Ask for an offer |
2x PCR TaqNova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR TaqNova-RED solution contains a reaction buffer, magnesium chloride, dNTPs and a thermostable Taq DNA polymerase. In order to set-up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water. This convenient 2x PCR TaqNova-RED master mix reduces the time required to set-up PCR reactions and decreases the possibility of contamination, particularly when preparing large numbers of reactions. Moreover, the master mix is supplemented with an inner dye and a density reagent, which allows for direct loading of PCR products to a gel.
Features and advantages
- Convenient – the TaqNova Polymerase in a ready-to-use mix
- Consistent results
- Suitable for a wide range of applications
- Recombinant enzyme of high purity
- Half-life of the enzyme is 45 minutes at 95°C
- Amplifies fragments of up to 5 kb
- Leaves ´A´ overhangs
- Facilitate high throughput PCR reaction set-up
- Reduced risk of contamination
- Direct gel loading
Applications
- Efficient amplification of short and medium size DNA sequences
- Routine PCR
- Diagnostic PCR
- TA cloning
- Multiplex PCR assays
Unit definition
One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.
2x PCR TaqNova-RED PCR Master Mix (RP85T) - Manual
Formulation
2x concentrated PCR reaction buffer, 4 mM MgCl2; 1.6 mM dNTPs mix (0.4 mM of each dNTP); 0.04 U/μl TaqNova DNA Polymerase, red inner dye and density reagent.
Storage conditions
Store all components at -20°C. After thawing, the product will remain
stable at +4°C for at least two months. Up to ten freeze/thaw cycles will not compromise product performance.
Shipping conditions
Shipping on dry or blue ice.
Quality control
Free of unspecific nucleases contamination. Extensively tested for PCR applications.
2x PCR TaqNova-RED PCR Master Mix (RP85T) - MSDS
2x PCR TaqNova-RED PCR Master Mix (RP85T) - L. TEZ606175
2x PCR TaqNova-RED PCR Master Mix (RP85T) - L. TEZ616074
2x PCR TaqNova-RED PCR Master Mix (RP85T) - L. TEZ616175
2x PCR TaqNova-RED PCR Master Mix (RP85T) - L. TEZ766074
2x PCR TaqNova-RED PCR Master Mix (RP85T) - L. TEZ855875
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Can procalcitonin be used as a clinical biomarker during bacterial, viral and parasitic infections in dogs?
Authors Erdal Matur, Banu Dokuzeylül, Mukaddes Özcan, Handan Çetinkaya, Murat Arslan, Erman Or, Songül Erhan, Ülker Çötelioğlu Products 2x PCR TaqNova-RED PCR Master Mix (RP85T), EXTRACTME DNA BLOOD KIT (EM05) – Discontinued Year 2021 Source Japanese Journal of Veterinary Research, 69(1), 5-17 -
New endosymbiotic relationships between Mucoromycota and Burkholderiaceae representatives
Authors Alicja Okrasińska, Aleksandra Bokus, Katarzyna Duk, Aleksandra Gęsiorska, Blanka Sokołowska, Aleksandra Miłobędzka, Marta Wrzosek, Julia Pawłowska Products 2x PCR TaqNova-RED PCR Master Mix (RP85T), EXTRACTME DNA CLEAN-UP KIT (EM07) – Discontinued, EXTRACTME GENOMIC DNA KIT (EM13) – Discontinued Year 2021 Source Applied and Environmental Microbiology, Jan 2021 -
Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold
Authors Nausika Betriu, Claire Jarrosson-Moral, Carlos E. Semino Products 2x PCR TaqNova-RED PCR Master Mix (RP85T), TRANSCRIPTME RNA Kit (RT31) Year 2020 Source Biomolecules 10(5) -
Lack of TLR4 modifies the miRNAs profile and attenuates inflammatory signaling pathways
Authors Juan R. Ureña-Peralta, Raúl Pérez-Moraga, Francisco García-García, Consuelo Guerri Products 2x PCR TaqNova-RED PCR Master Mix (RP85T) Year 2020 Source PLOS ONE 15 -
Antimicrobial Potential of Caffeic Acid against Staphylococcus aureus Clinical Strains
Authors Małgorzata Kępa, Maria Miklasińska-Majdanik, Robert D. Wojtyczka , Danuta Idzik, Konrad Korzeniowski, Joanna Smoleń-Dzirba, and Tomasz J.Wąsik Products 2x PCR TaqNova-RED PCR Master Mix (RP85T), 1 Kb HypeLadderIV Year 2018 Source Hindawi BioMed Research International Volume 2018, Article ID 7413504, 9 pages -
Effect of Replacing Soybean Meal by Raw or Extruded Pea Seeds on Growth Performance and Selected Physiological Parameters of the Ileum and Distal Colon of Pigs
Authors Anna Tuśnio, Marcin Taciak, Marcin Barszcz, Ewa Święch, Ilona Bachanek, Jacek Skomiał Products 2x PCR TaqNova-RED PCR Master Mix (RP85T) Year 2017 Source PLOS ONE -
In Vitro Antimicrobial Activity of Ethanolic Extract of Polish Propolis against Biofilm Forming Staphylococcus epidermidis Strains
Authors Robert D. Wojtyczka, Małgorzata Kępa, Danuta Idzik, Robert Kubina, Agata Kabała-Dzik, Arkadiusz Dziedzic, and Tomasz J.Wąsik Products 2x PCR TaqNova-RED PCR Master Mix (RP85T), EXTRACTME GENOMIC DNA KIT (EM13) – Discontinued, 1 Kb HyperLadder IV Year 2013 Source Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2013, Article ID 590703, 11 pages