48,00 € – 295,00 €2xPCR TaqNova-RED is a concentrated, ready-to-use PCR master mix with TaqNova polymerase, which facilitates an easy and rapid PCR reaction set up. The 2x PCR TaqNova-RED solution contains a reaction buffer, magnesium chloride, dNTPs and a thermostable Taq DNA polymerase. The 2x PCR TaqNova-RED is recommended for multiplex PCR assays.
- Additional information
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- Protocol & Manual
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- Quality & Safety
100 rxns, 1000 rxns
2x PCR TaqNova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR TaqNova-RED solution contains a reaction buffer, magnesium chloride, dNTPs and a thermostable Taq DNA polymerase. In order to set-up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water. This convenient 2x PCR TaqNova-RED master mix reduces the time required to set-up PCR reactions and decreases the possibility of contamination, particularly when preparing large numbers of reactions. Moreover, the master mix is supplemented with an inner dye and a density reagent, which allows for direct loading of PCR products to a gel.
Features and advantages
- Convenient – the TaqNova Polymerase in a ready-to-use mix
- Consistent results
- Suitable for a wide range of applications
- Recombinant enzyme of high purity
- Half-life of the enzyme is 45 minutes at 95°C
- Amplifies fragments of up to 5 kb
- Leaves ´A´ overhangs
- Facilitate high throughput PCR reaction set-up
- Reduced risk of contamination
- Direct gel loading
- Efficient amplification of short and medium size DNA sequences
- Routine PCR
- Diagnostic PCR
- TA cloning
- Multiplex PCR assays
One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.
Protocol & Manual
2x PCR TaqNova-RED PCR Master Mix (RP85T) - Manual
2x concentrated PCR reaction buffer, 4 mM MgCl2; 1.6 mM dNTPs mix (0.4 mM of each dNTP); 0.04 U/μl TaqNova DNA Polymerase, red inner dye and density reagent.
Store all components at -20°C. After thawing, the product will remain
stable at +4°C for at least two months. Up to ten freeze/thaw cycles will not compromise product performance.
Shipping on dry or blue ice.
Quality & Safety
Free of unspecific nucleases contamination. Extensively tested for PCR applications.
2x PCR TaqNova-RED PCR Master Mix (RP85T) - MSDS
2x PCR TaqNova-RED PCR Master Mix (RP85T) - CoA 2.2017 L.606172
2x PCR TaqNova-RED PCR Master Mix (RP85T) - CoA 1.2018 L.766173
2x PCR TaqNova-RED PCR Master Mix (RP85T) - CoA 2.2017 L.746472
Effect of Replacing Soybean Meal by Raw or Extruded Pea Seeds on Growth Performance and Selected Physiological Parameters of the Ileum and Distal Colon of Pigs
Authors: Anna Tuśnio, Marcin Taciak, Marcin Barszcz, Ewa Święch, Ilona Bachanek,Jacek Skomiał Products: 2x PCR TaqNova-RED PCR Master Mix (RP85T) Year: 2017 Source: PLOS ONE | DOI:10.1371/journal.pone.0169467 January 6, 2017
Antimicrobial Potential of Caffeic Acid against Staphylococcus aureus Clinical Strains
Authors: Małgorzata Kępa, Maria Miklasińska-Majdanik, Robert D. Wojtyczka, Danuta Idzik, Konrad Korzeniowski, Joanna Smoleń-Dzirba, and Tomasz J. Wąsik Products: 10x PCR RED master mix, 1 Kb HypeLadderIV Year: 2018 Source: Hindawi, BioMed Research International Volume 2018, Article ID 7413504, 9 pages