2x PCR TaqNova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR TaqNova-RED solution contains a reaction buffer, magnesium chloride, dNTPs and a thermostable Taq DNA polymerase. In order to set-up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water. This convenient 2x PCR TaqNova-RED master mix reduces the time required to set-up PCR reactions and decreases the possibility of contamination, particularly when preparing large numbers of reactions. Moreover, the master mix is supplemented with an inner dye and a density reagent, which allows for direct loading of PCR products to a gel.

Features and advantages

• Convenient – the TaqNova Polymerase in a ready-to-use mix
• Consistent results
• Suitable for a wide range of applications
• Recombinant enzyme of high purity
• Half-life of the enzyme is 45 minutes at 95°C
• Amplifies fragments of up to 5 kb
• Leaves ´A´ overhangs
• Facilitate high throughput PCR reaction set-up
• Reduced risk of contamination
• Direct gel loading

Applications

• Efficient amplification of short and medium size DNA sequences
• Routine PCR
• Diagnostic PCR
• TA cloning
• Multiplex PCR assays

Unit definition

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.