TaqNova-RED DNA Polymerase

TaqNova-RED DNA Polymerase

48,00 155,00 

TaqNova-RED polymerase with inert red dye for direct gel loading, which also facilitates accurate low volume pipetting and is an indicator of an enzyme addition

The thermostable TaqNova-RED DNA Polymerase catalyzes DNA synthesis in 5’→ 3’ direction, shows no 3’→ 5’ exonuclease activity, but demonstrates 5’→ 3’ exonuclease activity.

RP20R, RP100R

SKU: RP2/1 Categories: ,

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Unit

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Product Information

TaqNova-RED DNA Polymerase is a 94 kDa, recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus with an addition of inert, red dye. It facilitates accurate low volume pipetting and is an indicator of an enzyme addition. The dye does not affect the outcome of PCR adversely; the efficiency is identical as with the standard TaqNova DNA Polymerase.

The thermostable TaqNova-RED DNA Polymerase catalyzes DNA synthesis in 5’→ 3’ direction, shows no 3’→ 5’ exonuclease activity, but demonstrates 5’→ 3’ exonuclease activity.

Use of the TaqNova-RED DNA Polymerase decreases the risk of error occurrence during preparation of reactions e.g. omission of the polymerase or mixing inaccurate reagents. PCR product may be directly applied to a gel after amplification process is completed, with no need of mixing with loading buffer. It is less time consuming and facilitates a preparation of agarose or polyacrylamide gel electrophoresis. A fresh TAE/TBE buffer is recommended to use for the agarose gel preparation and electrophoretic processes. The dye does not interfere with any successive reactions (except reactions employing fluorescence techniques) and PCR products may be easily separated from the dye by any standard DNA purification method (e.g. PCR/DNA Clean-up Purification Kit).

Features and advantages

  • Consistent results
  • Suitable for a wide range of applications
  • Recombinant enzyme of high purity
  • Extreme yield with minimal amounts of enzyme and little optimization
  • Half-life of the enzyme is 45 minutes at 95°C
  • Amplifies fragments of up to 5 kb
  • Leaves ´A´ overhangs
  • Facilitate PCR reaction set-up
  • Direct gel loading

Applications

  • Efficient amplification of short and medium size DNA sequences
  • Routine PCR
  • Diagnostic PCR
  • TA cloning

Unit definition

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.

Protocol & Manual

TaqNova-RED DNA Polymerase - Manual

Technical Tips

Additional information

  • Both reaction buffers provided may be used with TaqNova-RED DNA polymerase. 10x TaqNova KCl buffer is recommended as first approach and for applications requiring high specificity. 10x TaqNova (NH4)2SO4 buffer is recommended for applications where high amplification efficiency is required (e.g. for multiple products’ amplification). Both buffers may be evaluated to determine the one that is most suitable for specific application.
  • The recommended concentration of TaqNova-RED DNA Polymerase in the reaction is 0.04 U/μl. If other polymerase concentrations are needed, magnesium ion concentration optimization may be required. The table below presents Mg2+ concentration calculations for the volume of 50 mM MgCl2 added to the reaction.
  • A minimal volume of the polymerase in a reaction is 1.5 μl / 50 μl, for the efficient sample loading to a gel.
  • PCR products may be applied directly onto a gel in a volume range of 5 – 15 μl with no need to mix them with a DNA loading dye. Electrophoresis, detection and visualization may be carried out in accordance with the standard procedures.

Storage buffer

20 mM Tris-HCl (pH 8.0, 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, inert dye and additives, 50% (v/v) glycerol

Reaction buffers

10x TaqNova KCl
100 mM Tris-HCl (pH 8.8, 25°C), 500 mM KCl, 0.8% (v/v) Nonidet P40

10x TaqNova (NH4)2SO4
750 mM Tris-HCl (pH 8.8, 25°C), 200 mM (NH4)2SO4, 0.1% (v/v) Tween 20

Storage conditions

Store all components at -20°C.

Shipping conditions

Shipping on dry or blue ice.

Quality & Safety

Quality control

Free of unspecific DNA nucleases. Functionality tested in PCR reaction.

TaqNova-RED DNA Polymerase - MSDS


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