Available Options:
| SKU | Unit | ||
|---|---|---|---|
 | RP710A | 1000 U ( 5 U/μl ) | Ask for an offer |
 | RP705A | 500 U ( 5U/μl ) | Ask for an offer |
TaqNova DNA Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The TaqNova DNA polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR conditions.
The enzyme catalyses DNA synthesis in a 5’→3’ direction, shows no 3’→ 5’ exonuclease activity, but has a 5’→ 3’ exonuclease activity.
Features:
- Recombinant thermostable DNA polymerases derived from Thermus aquaticus and overexpressed in E. coli.
- Extreme yields with minimal amounts of enzyme and little optimization.
- Increased sensitivity.
- Suitable for a wide range of applications.
- The half-life of the enzyme is 45 minutes at 95°C.
- Amplifies fragments of up to 5 kb.
- Leaves ´A´ overhangs.
Applications:
- Efficient amplification of short and medium size DNA sequences
- Routine PCR.
- Diagnostic PCR.
- Multiplex PCR.
- TA cloning.
Unit definition
One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.
TaqNova DNA Polymerase (RP7A) - Manual
Additional information
Both reaction buffers provided may be used with TaqNova DNA polymerase. 10 x TaqNova KCl buffer is recommended as first approach and for applications requiring high specificity. 10 x TaqNova (NH4)2SO4 buffer is recommended for applications where high sensitivity and amplification efficiency is required (e.g. for amplification of multiple DNA fragments).
Both buffers may be evaluated to determine the buffer most suitable for specific application.
Storage buffer
20 mM Tris-HCl (pH 8.0, 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.
Reaction buffers
10 x TaqNova KCl
100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% (v/v) Nonidet P40
10 x TaqNova (NH4)2SO4
750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% (v/v) Tween 20
Storage conditions
Store all components at -20°C.
Shipping conditions
Shipping on dry or blue ice.
Quality control
Free of nonspecific nucleases (DNases) contamination. Extensively tested in PCR reactions.
TaqNova DNA Polymerase (RP7A) – MSDS
TaqNova DNA Polymerase (RP7A) - L. TBZ725975
TaqNova DNA Polymerase (RP7A) - L. TBZ816274
TaqNova DNA Polymerase (RP7A) - L. TBZ846674
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Monitoring of bacterial pathogens at workplaces in power plant using biochemical and molecular methods
Authors Anna Ławniczek‑Wałczyk, Małgorzata Gołofit‑Szymczak, Marcin Cyprowski, Agata Stobnicka, Rafał L. Górny Products EXTRACTME DNA BACTERIA KIT (EM02) – Discontinued, TaqNova DNA Polymerase (RP7A) Year 2017 Source Int Arch Occup Environ Health (2017) 90:285–295 -
The Effect of Leonurus sibiricus Plant Extracts on Stimulating Repair and Protective Activity against Oxidative DNA Damage in CHO Cells and Content of Phenolic Compounds
Authors Przemysław Sitarek, Ewa Skała, Halina Wysokińska, Marzena Wielanek, Janusz Szemraj, Monika Toma, and Tomasz Śliwiński Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued, TaqNova DNA Polymerase (RP7A) Year 2016 Source Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2016, Article ID 5738193, 11 pages -
Antioxidant and DNA Repair Stimulating Effect of Extracts from Transformed and Normal Roots of Rhaponticum carthamoides against Induced Oxidative Stress and DNA Damage in CHO Cells
Authors Ewa Skała, Przemysław Sitarek, Marek Różalski, Urszula Krajewska, Janusz Szemraj, Halina Wysokińska, and Tomasz Śliwiński Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued, LM Agarose (AG43) – Discontinued, TaqNova DNA Polymerase (RP7A) Year 2016 Source Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2016 -
Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production
Authors Ewa Skała, Renata Grąbkowska, Przemysław Sitarek, Łukasz Kuźma, Andrzej Błauż, Halina Wysokińska Products TaqNova DNA Polymerase (RP7A), Deoxyribonucleotides (dNTPs), Primers Year 2015 Source Plant Cell Tiss Organ Cult (2015) 123:83–98 -
Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives
Authors Ewa Skała, Agnieszka Kicel, Monika A. Olszewska, Anna K. Kiss, and Halina Wysokińska Products TaqNova DNA Polymerase (RP7A), Deoxyribonucleotides (dNTPs), Primers Year 2015 Source Hindawi Publishing Corporation BioMed Research International Volume 2015, Article ID 181098, 11 pages -
Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens
Authors Renata Grąbkowska, Przemysław Sitarek, Halina Wysokińska Products TaqNova DNA Polymerase (RP7A), Primers Year 2014 Source Acta Physiol Plant (2014) 36:1661–1672