TaqNova DNA Polymerase (RP7)

TaqNova DNA Polymerase (RP7)

43,00 251,00 

TaqNova DNA Polymerase suited to a wide range of applications, fast and very efficient; universal and easy-to-use; half-life of the enzyme is 45 minutes at 95°C; shows 5’→3’ exonuclease activity; does not have 3’→5’ exonuclease activity; adds A on the 3’ ends.
RP7, RP702, RP702A, RP705, RP705A, RP710, RP710A, RP725, RP725A

SKU: RP7 Categories: ,

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Product Information

TaqNova DNA Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The TaqNova DNA polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR conditions.

The enzyme catalyses DNA synthesis in a 5’→3’ direction, shows no 3’→ 5’ exonuclease activity, but has a 5’→ 3’ exonuclease activity.

Features:

  • Recombinant thermostable DNA polymerases derived from Thermus aquaticus and overexpressed in E. coli.
  • Extreme yields with minimal amounts of enzyme and little optimization.
  • Increased sensitivity.
  • Suitable for a wide range of applications.
  • The half-life of the enzyme is 45 minutes at 95°C.
  • Amplifies fragments of up to 5 kb.
  • Leaves ´A´ overhangs.

Applications:

  • Efficient amplification of short and medium size DNA sequences
  • Routine PCR.
  • Diagnostic PCR.
  • Multiplex PCR.
  • TA cloning.

Unit definition

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.

Protocol & Manual

TaqNova DNA Polymerase (RP7) - Manual

Technical Tips

Additional information

Both reaction buffers provided may be used with TaqNova DNA polymerase. 10 x TaqNova KCl buffer is recommended as first approach and for applications requiring high specificity. 10 x TaqNova (NH4)2SO4 buffer is recommended for applications where high sensitivity and amplification efficiency is required (e.g. for amplification of multiple DNA fragments).

Both buffers may be evaluated to determine the buffer most suitable for specific application.

Storage buffer

20 mM Tris-HCl (pH 8.0, 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.

Reaction buffers

10 x TaqNova KCl
100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% (v/v) Nonidet P40

10 x TaqNova (NH4)2SO4
750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% (v/v) Tween 20

Storage conditions

Store all components at -20°C.

Shipping conditions

Shipping on dry or blue ice.

Quality & Safety

Quality control

Free of nonspecific nucleases (DNases) contamination. Extensively tested in PCR reactions.

TaqNova DNA Polymerase (RP7) - MSDS


TaqNova DNA Polymerase (RP7) - CoA 2.2017 L.856372

TaqNova DNA Polymerase (RP7) - CoA 2.2017 L.866372

TaqNova DNA Polymerase (RP7) - CoA 2.2017 L.775872

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