TaqNova DNA Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The TaqNova DNA polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR conditions.
The enzyme catalyses DNA synthesis in a 5’→3’ direction, shows no 3’→ 5’ exonuclease activity, but has a 5’→ 3’ exonuclease activity.
- Recombinant thermostable DNA polymerases derived from Thermus aquaticus and overexpressed in E. coli.
- Extreme yields with minimal amounts of enzyme and little optimization.
- Increased sensitivity.
- Suitable for a wide range of applications.
- The half-life of the enzyme is 45 minutes at 95°C.
- Amplifies fragments of up to 5 kb.
- Leaves ´A´ overhangs.
- Efficient amplification of short and medium size DNA sequences
- Routine PCR.
- Diagnostic PCR.
- Multiplex PCR.
- TA cloning.
One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.
TaqNova DNA Polymerase ( 2U/μl ) - Discontinued - Manual
Both reaction buffers provided may be used with TaqNova DNA polymerase. 10 x TaqNova KCl buffer is recommended as first approach and for applications requiring high specificity. 10 x TaqNova (NH4)2SO4 buffer is recommended for applications where high sensitivity and amplification efficiency is required (e.g. for amplification of multiple DNA fragments).
Both buffers may be evaluated to determine the buffer most suitable for specific application.
20 mM Tris-HCl (pH 8.0, 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.
10 x TaqNova KCl
100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% (v/v) Nonidet P40
10 x TaqNova (NH4)2SO4
750 mM Tris-HCl (pH 8.8 at 25°C), 200 mM (NH4)2SO4, 0.1% (v/v) Tween 20
Store all components at -20°C.
Shipping on dry or blue ice.
Free of nonspecific nucleases (DNases) contamination. Extensively tested in PCR reactions.