TaqNova DNA-free Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The TaqNova DNA-free Polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR condition. The enzyme catalyzes DNA synthesis in a 5’ → 3’ direction, shows no 3’ → 5’ exonuclease activity, but has a 5’ → 3’ exonuclease activity.

TaqNova DNA-free Polymerase is highly purified from DNA contaminants, enabling amplification of very conserved sequences (e.g. bacterial 16S rRNA region) without risk of false positive PCR results.

Features and advantages:

• Extreme yield with minimal amounts of enzyme and little optimization
• Increased sensitivity
• Suitable for a wide range of applications
• Consistent results
• High-purity recombinant enzyme from DNA contaminants
• The half-life of the enzyme is 45 minutes at 95°C
• Amplifies fragments of up to 5 kb
• Leaves ´A´ overhangs

Applications:

• Efficient amplification of conserved sequences
  (e.g. bacterial 16S rRNA region)
• Routine PCR
• Diagnostic PCR
• Multiplex PCR
• TA cloning

Unit definition

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 72°C in a 50 μl reaction