TaqNova DNA-free Polymerase (RP10)

TaqNova DNA-free Polymerase (RP10)

121,00 498,00 

TaqNova DNA-free Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The TaqNova DNA-free Polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR condition. The enzyme catalyzes DNA synthesis in a 5’ → 3’ direction, shows no 3’ → 5’ exonuclease activity, but has a 5’ → 3’ exonuclease activity.
RP10, RP1002, RP1002-S, RP1010

SKU: RP10 Categories: ,

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TaqNova DNA-free Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The TaqNova DNA-free Polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR condition. The enzyme catalyzes DNA synthesis in a 5’ → 3’ direction, shows no 3’ → 5’ exonuclease activity, but has a 5’ → 3’ exonuclease activity.

TaqNova DNA-free Polymerase is highly purified from DNA contaminants, enabling amplification of very conserved sequences (e.g. bacterial 16S rRNA region) without risk of false positive PCR results.

Features and advantages:

  • Extreme yield with minimal amounts of enzyme and little optimization
  • Increased sensitivity
  • Suitable for a wide range of applications
  • Consistent results
  • High-purity recombinant enzyme from DNA contaminants
  • The half-life of the enzyme is 45 minutes at 95°C
  • Amplifies fragments of up to 5 kb
  • Leaves ´A´ overhangs

Applications:

  • Efficient amplification of conserved sequences
    (e.g. bacterial 16S rRNA region)
  • Routine PCR
  • Diagnostic PCR
  • Multiplex PCR
  • TA cloning

Unit definition

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 72°C in a 50 μl reaction

TaqNova DNA-free Polymerase (RP10) - Manual

Storage buffer

20 mM Tris-HCl (pH 8.0 at 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.

10 x TaqNova DNA-free Reaction Buffer

100 mM Tris-HCl (pH 8.3 at 25°C), 500 mM KCl

Storage conditions

Store all components at -20°C.

Shipping conditions

Shipping on dry or blue ice.

Quality control

  • Purity > 95% determined by densitometry of SDS-PAGE
  • Functionality of the enzyme tested in PCR reaction with human DNA
  • Free of nonspecific DNase contamination
  • DNA contamination is evaluated by qPCR reaction with primers specific for 16S rDNA region, less than 1 E. coli genome is detected in 1 U of enzyme

TaqNova DNA-free Polymerase (RP10) - MSDS


TaqNova DNA-free Polymerase (RP10) - CoA L. TLZ716274

TaqNova DNA-free Polymerase (RP10) - L. 735775