PROTEINASE K NGS Grade

Proteinase K NGS Grade is a broad spectrum endopeptidase with a very high specific activity developed for most demanding applications. Additional purification technology results in its significantly increased solubility (2.5 fold), increased specific activity, and remarkable purity with DNA content ≤0.1 pg/mg. The product is available as specially formulated for smooth aliquoting lyophilized powder. Proteinase K NGS Grade is free of exonucleases, endonucleases, and ribonucleases. Also, check our PROTEINASE K Molecular Biology Grade
RP100, RP100N, RP101N, RP102N, RP103N, RP107B

Available Options:
SKU Size
Proteinase K NGS bulk RP103N bulk Ask for an offer
RP100N 100 mg Ask for an offer
Proteinase K MB Grade 10g RP102N 1000 mg Ask for an offer

Proteinase K from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad-spectrum endopeptidase with a very high specific activity. It is widely used for the digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and the presence of SDS. This recombinant enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product. An extra purification step results in significantly increased solubility (2.5 fold), increased specific activity, and decreased DNA content, compared to our Molecular Biology Grade product.

Below, you can see the comparison of Proteinase K MBG and NGS Grade (Table 1 ).

Proteinase K comparison

 

Features

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • High activity and exceptional purity (Molecular Biology Grade & NGS Grade).
  • Active at high temperatures (up to 56°C) and under denaturing conditions (e.g. in the presence of urea and/or SDS), which makes it ideal for digesting proteins in a variety of applications.
  • Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Available as a lyophilized powder.

Applications:

  • Extraction of DNA and RNA from different starting materials.
  • Removal of DNases and RNases during nucleic acid isolation.
  • Purification of samples contaminated with different protein
  • Automated isolation stations

Units

One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing a color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.

 

 

PROTEINASE K NGS Grade - Product Specification

PROTEINASE K NGS Grade - Flyer

Proteinase K – Lyophilized powder, NGS Grade

  • DNA content

≤ 0.1 pg/mg by qPCR

 

  • Solubility in water

≥ 50 mg/ml

 

  • Activity

≥ 35 U/mg lyophilizate
≥ 45 U/mg protein

 

  • Protein content

≥ 70% Protein content is determined by measuring absorbance at 280 nm.

 

Proteinase K Stock solution preparation

  • 20-50 mg/ml solutions: use purified water for immediate use; or 50% glycerol in purified water (v/v) for long-term storage at -20˚C.
  • < 20 mg/ml solutions: use 50 mM Tris-HCl, pH = 7.8, 3 mM CaCl2 for immediate use; or 50 mM Tris-HCl, pH = 7.8, 3 mM CaCl2 , 50% glycerol for long-term storage at -20˚C.

PROTEINASE K NGS Grade - MSDS


PROTEINASE K NGS Grade - CoA