20,00 € – 273,00 €Proteinase K Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad spectrum endopeptidase with a very high specific activity. Proteinase K Molecular Biology Grade is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS. As a result, this enzyme is widely using for digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K Molecular Biology Grade is free of exonucleases, endonucleases, and ribonucleases. Also check our Proteinase K NGS Grade
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PROTEINASE K Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease
This recombinant enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.
- Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
- High activity and exceptional purity .
- Active at high temperatures (up to 56°C) and denaturing conditions (e.g. in the presence of urea and/or SDS), what makes it ideal for digesting proteins in variety of applications.
- Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0).
- Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
- Available as powder, lyophilized “cake” or liquid.
- Extraction of DNA and RNA from different starting materials.
- Purification of target material from contaminating proteins.
- Removal of DNases and RNases during nucleic acids isolation.
One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.
PROTEINASE K Molecular Biology Grade - Description (powder)
PROTEINASE K Molecular Biology Grade - Description (solution)
PROTEINASE K Molecular Biology Grade - Flyer (Chinese)
Proteinase K MB Grade – Lyophilized powder
- Solubility in water
≥ 20 mg/ml
≥ 30 U/mg lyophilizate
≥ 40 U/mg protein
- Protein content
≥ 70% Protein content is determined by measuring absorbance at 280 nm.
- DNA content
≤ 10 pg/mg by qPCR
Proteinase K MB Grade – Solution
- Proteinase K concentration
≥ 800 U/ml
- DNA content
≤ 0.25 pg/U by qPCR
PROTEINASE K Molecular Biology Grade - MSDS
PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.745872
PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.666272
PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.676172
PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.776372
PROTEINASE K Molecular Biology Grade - CoA 1.2016 L.626571
PROTEINASE K Molecular Biology Grade - CoA 1.2015 L.825770
PROTEINASE K Molecular Biology Grade - CoA 1.2015 L.575870
PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.646072
PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.667472
PROTEINASE K Molecular Biology Grade - CoA L.696672
Influence of active compounds on the degradation of polylactide biocomposites
Authors Magdalena Stepczyńska Products Proteinase K Year 2019 Source Polimery, 2019, Vol. 64 Issue 6, p410-416. 7p.
Enzymatic degradation of bacteriostatic polylactide composites
Authors Agnieszka Richert, Ewa Olewnik-Kruszkowska, Edyta Adamska, Iwona Tarach Products Proteinase K Year 2019 Source International Biodeterioration & Biodegradation, Volume 142, 2019, Pages 103-108
Broadening the tools for studying sand fly breeding habitats: A novel molecular approach for the detection of phlebotomine larval DNA in soil substrates
Authors I.A. Giantsis, A. Chaskopoulou, Products Proteinase K Year 2019 Source Acta Tropica, Volume 190, 2019, Pages 123-128
Enzymatic degradation of flax-fibers reinforced polylactide
Authors Magdalena Stepczyńska, Piotr Rytlewski Products Proteinase K Year 2018 Source Elsevier
Flax fibres reinforced polylactide modified by ionizing radiation
Authors Piotr Rytlewski, Magdalena Stepczyńska, Uwe Gohs, Rafał Malinowski, Bogusław Budner, Marian Żenkiewicz Products Proteinase K Year 2018 Source Elsevier
A comparative analysis of mass losses of some aliphatic polyesters upon enzymatic degradation
Authors Marian Żenkiewicz, Agnieszka Richert, Rafał Malinowski, Krzysztof Moraczewski Products Proteinase K Year 2012 Source Elsevier
Some composting and biodegradation effects of physically or chemically
Authors Marian Żenkiewicz, Rafał Malinowski, Piotr Rytlewski, Agnieszka Richert, Wanda Sikorska, Katarzyna Krasowska Products Proteinase K Year 2011 Source Elsevier
Blirt’s Proteinase K MBG is interchangeable with comparators without dose adjustments.
Declared activity for all shown suppliers is ≥30 U/mg (blue line).
Blirt’s Proteinase K MBG shelf life is guaranteed up to 2 years (storage at -20°C).
Recommended storage of MBG Proteinase K Solution is -20°C
Temporary exposures to temperature (tested at +37 ° C) changes e.g. during transportation or experiments bring no differences in protein activity.
Automation of the Proteinase K MBG Production results in minimal batch to batch activity variation.
Consistency in the product characteristics in all delivered batches guarantees reliable experiments results and stable working conditions.
Blue line represents declared minimum activity (≥ 30 U/mg). Batch-to-batch variation is 3,0% (with measurement error ± 1-6%).