PROTEINASE K Molecular Biology Grade

PROTEINASE K Molecular Biology Grade

30,00 9 000,00 

Proteinase K from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad spectrum endopeptidase with a very high specific activity. Proteinase K Molecular Biology Grade is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS. As a result, Proteinase K is widely using for digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K Molecular Biology Grade is free of exonucleases, endonucleases, and ribonucleases. Also check our Proteinase K NGS Grade
RP100, RP100B, RP101B, RP102B, RP103B-5, RP103B-10, RP103B-50, RP103B, RP103B-C, RP107B, RP107B-1, RP107B-5

SKU: RP Categories: ,

Available Options:

Additional information

Form

Lyophilized powder, Solution, Cake

Size

100 mg, 250 mg, 1000 mg, bulk, 5 g, 10 g, 50 g, 1 ml, 5 ml

Product Information

PROTEINASE K Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease

This recombinant enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.

Features

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • High activity and exceptional purity (Molecular Biology Grade & NGS Grade).
  • Active at high temperatures (up to 56°C) and denaturing conditions (e.g. in the presence of urea and/or SDS), what makes it ideal for digesting proteins in variety of applications.
  • Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Available as powder, lyophilized “cake” or liquid.

Applications:

  • Extraction of DNA and RNA from different starting materials.
  • Purification of target material from contaminating proteins.
  • Removal of DNases and RNases during nucleic acids isolation.

Units

One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.

Protocol & Manual

PROTEINASE K Molecular Biology Grade - Description (powder)

PROTEINASE K Molecular Biology Grade - Description (solution)

PROTEINASE K Molecular Biology Grade - Flyer (Chinese)

Technical Tips

Proteinase K – Lyophilized powder, Molecular Biology Grade

  • Solubility in water

≥ 20 mg/ml

  • Activity

≥ 30 U/mg lyophilizate
≥ 40 U/mg protein

  • Protein content

≥ 70% Protein content is determined by measuring absorbance at 280 nm.

  • DNA content

≤ 10 pg/mg by qPCR

Proteinase K – Solution, Molecular Biology Grade

  • Proteinase K concentration

20 mg/ml

  • Activity

≥ 800 U/ml

  • DNA content

≤ 0.25 pg/U by qPCR

FAQ

Quality & Safety

PROTEINASE K Molecular Biology Grade - MSDS


PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.745872

PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.666272

PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.676172

PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.776372

PROTEINASE K Molecular Biology Grade - CoA 1.2016 L.626571

PROTEINASE K Molecular Biology Grade - CoA 1.2015 L.825770

PROTEINASE K Molecular Biology Grade - CoA 1.2015 L.575870

PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.646072

PROTEINASE K Molecular Biology Grade - CoA 3.2017 L.667472

PROTEINASE K Molecular Biology Grade - CoA L.696672

Literature