PROTEINASE K Molecular Biology Grade

PROTEINASE K Molecular Biology Grade

20,00 273,00 

Proteinase K Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad spectrum endopeptidase with a very high specific activity. Proteinase K Molecular Biology Grade is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS. As a result, this enzyme is widely using for digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K Molecular Biology Grade is free of exonucleases, endonucleases, and ribonucleases. Also check our Proteinase K NGS Grade
RP100, RP100B, RP101B, RP102B, RP103B-5, RP103B-10, RP103B-50, RP103B, RP103B-C, RP107B, RP107B-1, RP107B-5

Available Options:

PROTEINASE K Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease

This recombinant enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.

Features

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • High activity and exceptional purity .
  • Active at high temperatures (up to 56°C) and denaturing conditions (e.g. in the presence of urea and/or SDS), what makes it ideal for digesting proteins in variety of applications.
  • Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Available as powder, lyophilized “cake” or liquid.

Applications:

  • Extraction of DNA and RNA from different starting materials.
  • Purification of target material from contaminating proteins.
  • Removal of DNases and RNases during nucleic acids isolation.

Units

One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.

BLIRT Proteinase K Molecular Biology Grade

Activity

Proteinase K MBG - Activity

Blirt’s Proteinase K MBG is interchangeable with comparators without dose adjustments.
Declared activity for all shown suppliers is ≥30 U/mg (blue line).

Stability

Proteinase K MBG activity after up to 2 years storage at -20°C is guaranteed by Certificate of Analysis, additionally performed experiments revealed only <14% activity loss after 3 years storage at -20°C (and it is still above-declared ≥30U/mg). It is a very important feature for molecular biology kits manufacturers.

 

Proteinase K MBG Solution storage at -20°C is recommended.
No significant differences in protein activity at +37°C after 18 months have been proven.
Proteinase K remains full activity in spite of temporary exposures to temperature changes e.g. during transportation or experiments.

 

Consistency

Automation of the Proteinase K MBG Production results in minimal batch to batch activity variation.

Consistency in the product characteristics in all delivered batches guarantees reliable experiments results and stable working conditions.

Blue line represents declared minimum activity (≥ 30 U/mg). Batch-to-batch variation is 3,0% (with measurement error ± 1-6%).

PROTEINASE K Molecular Biology Grade - Description (powder)

PROTEINASE K Molecular Biology Grade - Description (solution)

PROTEINASE K Molecular Biology Grade - Flyer (Chinese)

Proteinase K MB Grade – Lyophilized powder

  • Solubility in water

≥ 20 mg/ml

  • Activity

≥ 30 U/mg lyophilizate
≥ 40 U/mg protein

  • Protein content

≥ 70% Protein content is determined by measuring absorbance at 280 nm.

  • DNA content

≤ 10 pg/mg by qPCR

Proteinase K MB Grade – Solution

  • Proteinase K concentration

20 mg/ml

  • Activity

≥ 800 U/ml

  • DNA content

≤ 0.25 pg/U by qPCR

PROTEINASE K Molecular Biology Grade - MSDS - Powder

PROTEINASE K Molecular Biology Grade - MSDS - Solution


PROTEINASE K Molecular Biology Grade - Powder CoA L:NAE886174

PROTEINASE K Molecular Biology Grade - Powder CoA L:NAE855875

PROTEINASE K Molecular Biology Grade - Powder CoA L:NAE795775

PROTEINASE K Molecular Biology Grade - Powder CoA L:NAE566073

PROTEINASE K Molecular Biology Grade - Powder CoA L:876174

PROTEINASE K Molecular Biology Grade - Solution CoA L:NBE575875-F1

PROTEINASE K Molecular Biology Grade - Solution CoA L:NBE795775-F1

PROTEINASE K Molecular Biology Grade - Cake CoA L:736473-C1