Proteinase K - one of the most commonly used enzymes in molecular biology.Perfect choice for protein digestion in various applications.
- BLIRT - expert in Proteinase K production for over 15 years.
- Certified manufacturing process ISO 13485 resulting in the highest quality product with a very low batch-to-batch variability.
- Large quantities, the possibility of white labeling, and portioning service - liquid form in the amount at the customer's request (up to several million samples monthly).
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- Product Information
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- Protocol & Manual
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PROTEINASE K Molecular Biology Grade
Proteinase K Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad-spectrum endopeptidase with a very high specific activity.
Recombinant Proteinase K enzyme is expressed in Pichia pastoris, and undergoes extensive purification to yield the highest quality product.
Proteinase K Molecular Biology Grade (PCR Grade) is active under a wide range of reaction conditions, including elevated temperatures and the presence of SDS. As a result, this enzyme is widely using for the digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA.
Proteinase K Molecular Biology Grade is free of exonucleases, endonucleases, and ribonucleases. Also check our Proteinase K NGS Grade
- Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
- High activity and exceptional purity.
- Active at high temperatures (up to 56 °C) and denaturing conditions (e.g. in the presence of urea and/or SDS), what makes it ideal for digesting proteins in variety of applications.
- Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0).
- Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
- Available as powder, lyophilized “cake” or liquid.
- Extraction of DNA and RNA from different starting materials.
- Purification of target material from contaminating proteins.
- Removal of DNases and RNases during nucleic acids isolation.
One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.
Blirt’s Proteinase K MBG is interchangeable with comparators without dose adjustments.
Declared activity for all shown suppliers is ≥30 U/mg (blue line).
Proteinase K MBG Powder activity after up to 2 years storage at -20°C is guaranteed by Certificate of Analysis, additionally performed experiments revealed only <14% activity loss after 3 years storage at -20°C (and it is still above-declared ≥30U/mg). It is a very important feature for molecular biology kits manufacturers.
Automation of the Proteinase K MBG Production results in minimal batch to batch activity variation.
Consistency in the product characteristics in all delivered batches guarantees reliable experiments results and stable working conditions.
Blue line represents declared minimum activity (≥ 30 U/mg). Batch-to-batch variation is 3,0% (with measurement error ± 1-6%).
Proteinase K MB Grade – Lyophilized powder
- Solubility in water
≥ 20 mg/ml
≥ 30 U/mg lyophilizate
≥ 40 U/mg protein
- Protein content
≥ 70% Protein content is determined by measuring absorbance at 280 nm.
- DNA content
≤ 10 pg/mg by qPCR
Stock solution preparation
- 20 mg/ml solutions: use purified water for immediate use.
- 20 – 50 mg/ml solutions: use 50 mM Tris-HCl, pH = 7.5-8.0, 1-5 mM Ca2+ (calcium chloride, calcium acetate) for immediate use; or 10 mM Tris-HCl, pH = 7.5-8.0, 1-5 mM Ca2+ (calcium chloride, calcium acetate), 50% glycerol for long-term storage.
Proteinase K MB Grade – Solution
- Proteinase K concentration
≥ 800 U/ml
- DNA content
≤ 200 pg/ml by qPCR
PROTEINASE K Molecular Biology Grade - Product Specification (Powder)
PROTEINASE K Molecular Biology Grade - Product Specification (Solution)
PROTEINASE K Molecular Biology Grade - Flyer (Chinese)
What is the recommended reconstituted concentration of Proteinase K?
Proteinase K MB and NGS grade is water soluble at the optimal concentration of 20 and 50 mg/ml, respectively. We also recommend to prepare solutions using 10-50 mM Tris-HCl, pH = 7.5-8.0, 1-5 mM Ca2+ (calcium chloride, calcium acetate) for immediate use or 10 mM Tris-HCl, pH = 7.5-8.0, 1-5 mM Ca2+ (calcium chloride, calcium acetate), 50% glycerol for long-term storage.
At what temperature is Proteinase K active?
Proteinase K activity increases with temperature, which is up to 56 °C.
How to inactivate Proteinase K?
Heating proteinase K to 95°C for 10 minutes incompletely inactivates it. To our knowledge, Proteinase K cannot be completely heat-inactivated. Protease inhibitors such as PMSF and AEBSF can also be used to help inactivate Proteinase K.
What is the optimal pH for using Proteinase K?
Proteinase K can be used in a wide range of pH levels from 4.0 to 12.5, with its optimum at 7.5-8.0.
2. Can Proteinase K be shipped at room temperature? How stable is Proteinase K when exposed to higher temperatures during shipment?
Although we recommend storing the Proteinase K in powder at -20°C, its shipment is at ambient temperature. Our stability tests display no decline of Proteinase K activity when exposed to a short-term temperature rise.
PROTEINASE K Molecular Biology Grade - Powder - MSDS
PROTEINASE K Molecular Biology Grade - Solution - MSDS
PROTEINASE K Molecular Biology Grade - Powder - CoA
PROTEINASE K Molecular Biology Grade - Solution -CoA
Influence of active compounds on the degradation of polylactide biocomposites
Authors Magdalena Stepczyńska Products Proteinase K Year 2019 Source Polimery, 2019, Vol. 64 Issue 6, p410-416. 7p.
Enzymatic degradation of bacteriostatic polylactide composites
Authors Agnieszka Richert, Ewa Olewnik-Kruszkowska, Edyta Adamska, Iwona Tarach Products Proteinase K Year 2019 Source International Biodeterioration & Biodegradation, Volume 142, 2019, Pages 103-108
Broadening the tools for studying sand fly breeding habitats: A novel molecular approach for the detection of phlebotomine larval DNA in soil substrates
Authors I.A. Giantsis, A. Chaskopoulou, Products Proteinase K Year 2019 Source Acta Tropica, Volume 190, 2019, Pages 123-128
Enzymatic degradation of flax-fibers reinforced polylactide
Authors Magdalena Stepczyńska, Piotr Rytlewski Products Proteinase K Year 2018 Source Elsevier
Flax fibres reinforced polylactide modified by ionizing radiation
Authors Piotr Rytlewski, Magdalena Stepczyńska, Uwe Gohs, Rafał Malinowski, Bogusław Budner, Marian Żenkiewicz Products Proteinase K Year 2018 Source Elsevier
A comparative analysis of mass losses of some aliphatic polyesters upon enzymatic degradation
Authors Marian Żenkiewicz, Agnieszka Richert, Rafał Malinowski, Krzysztof Moraczewski Products Proteinase K Year 2012 Source Elsevier
Some composting and biodegradation effects of physically or chemically
Authors Marian Żenkiewicz, Rafał Malinowski, Piotr Rytlewski, Agnieszka Richert, Wanda Sikorska, Katarzyna Krasowska Products Proteinase K Year 2011 Source Elsevier