The M50pz molecular weight DNA ladder is designed for determining the size of short and medium PCR products and dsDNA fragments in the 50 - 1000 bp range.
The M50pz DNA Ladder is a mixture of specially selected, purified, blunt-endedPCR products. The DNA Ladder gives a clear band pattern, with two bands of increased intensity employed as reference points.
Ready-to-use version is pre-mixed with 6x GREEN DNA loading buffer.
The M50pz molecular weight DNA ladder is designed for determining the size of short and medium PCR products and dsDNA fragments in the 50 – 1000 bp range. The M50pz DNA Ladder is a mixture of specially selected, purified, blunt-ended PCR products. The DNA Ladder gives a clear band pattern, with two bands of increased intensity employed as reference points. A DNA loading buffer (6x GREEN) is provided with each DNA ladder. The loading buffer contain Ficoll® 400, which facilitates rapid sinking to the bottom of the well in the agarose or polyacrylamide gel. The solution protects DNA thanks to the presence of EDTA, which inactivates nucleases and other enzymes by binding the divalent metal ions crucial to their activity. The 6x GREEN solution contains two different dyes which migrate in the electrophoretic field differently from the standard size PCR products, ensuring that they do not hinder the analysis by image obscuration. The orange G dye corresponds to 50 bp and xylene cyanol FF to 4000 bp PCR products in a 1% agarose gel.
The M50pz DNA Ladder is also supplied in a ready-to-use version pre-mixed with 6x GREEN DNA loading buffer.
Features and advantages
- Allows precise determination of short and medium sized DNA fragments
- Bands at 50 bp and 250 bp have a higher intensity to allow easy identification
- Sharp bands are obtained after electrophoresis
- Suitable for gels stained with ethidium bromide, SYBR Green or other stain for DNA visualization
- Ready-to-use: direct gel loading
DNA fragment lengths (bp):
1000, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100, 50
M50pz DNA Ladder ready-to-use (MR201) - Manual
- For the best results, apply approx. 10 μl of the DNA ladder per well.
- Mix 1 volume of DNA loading dye with 5 volumes of analyzed DNA and/or DNA ladder before application.
6x GREEN DNA Loading Buffer
20 mM Tris-HCl (pH 7.6), 60 mM EDTA, 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400
Keep at +4°C or room temperature for short-term or at -20°C for long-term storage.
The DNA ladders have been tested in various kinds of agarose and polyacrylamide gels. The absence of nucleases has been confirmed by the relevant QC procedures.