M50-1500 Fast DNA Ladder (ready-to-use) (MR27)

M50-1500 Fast DNA Ladder (ready-to-use) (MR27)

54,00 

The M50-1500 Fast DNA Ladder is specially designed for fast and accurate sizing and quantification of double-stranded DNA in 48-well (or 96-well) gels as well as in conventional agarose gels. The M50-1500 Fast DNA Ladder is a mixture of specially selected, purified PCR products: 1500, 800, 400, 200, 50 mixed in equal quantities.

MR27

SKU: MR27 Categories: ,

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The M50-1500 Fast DNA Ladder is specially designed for fast and accurate sizing and quantification of double-stranded DNA in 48-well (or 96-well) gels as well as in conventional agarose gels. The M50-1500 Fast DNA Ladder is a mixture of specially selected, purified PCR products: 1500, 800, 400, 200, 50 mixed in equal quantities. The ladder bands are easily resolved after an 8-14 min run on appropriate agarose gels in a short 10-20 mm linear separation distance and visualized by ethidium bromide or SYBR ® Green I staining. The Ladder is premixed with a loading buffer and can be applied directly onto an agarose gel.

Features and advantages

  • Allows fast and precise determination of short and medium sized DNA fragments
  • Sharp bands are obtained after electrophoresis
  • Suitable for gels stained with ethidium bromide, SYBR Green or other stain for DNA visualization
  • Ready-to-use: direct gel loading

DNA fragment lengths (bp):

1500, 800, 400, 200, 50

M50-1500 Fast DNA Ladder (ready-to-use) (MR27) - Manual

Usage

  • For the best results, apply approx. 10 μl of the DNA ladder per well.
  • Mix 1 volume of DNA loading dye with 5 volumes of analyzed DNA before application

6x FastLadder Loading Dye

10 mM Tris-HCl (pH 7.6), 60 mM EDTA, 0.03% bromophenol blue and 60% glycerol

Storage conditions

Keep at +4°C or room temperature for short-term or at -20°C for long-term storage.

Quality control

The DNA ladders have been tested in various kinds of agarose and polyacrylamide gels. The absence of nucleases has been confirmed by the relevant QC procedures.

M50-1500 Fast DNA Ladder (ready-to-use) (MR27) - MSDS