The M50-1500 Fast DNA Ladder is specially designed for fast and accurate sizing and quantification of double-stranded DNA in 48-well (or 96-well) gels as well as in conventional agarose gels. The M50-1500 Fast DNA Ladder is a mixture of specially selected, purified PCR products: 1500, 800, 400, 200, 50 mixed in equal quantities.
Available Options:
| SKU | Quantity | ||
|---|---|---|---|
 | MR27 | 50-100 lanes (ready-to-use) | Ask for an offer |
The M50-1500 Fast DNA Ladder is specially designed for fast and accurate sizing and quantification of double-stranded DNA in 48-well (or 96-well) gels as well as in conventional agarose gels. The M50-1500 Fast DNA Ladder is a mixture of specially selected, purified PCR products: 1500, 800, 400, 200, 50 mixed in equal quantities. The ladder bands are easily resolved after an 8-14 min run on appropriate agarose gels in a short 10-20 mm linear separation distance and visualized by ethidium bromide or SYBR ® Green I staining. The Ladder is premixed with a loading buffer and can be applied directly onto an agarose gel.
Features and advantages
- Allows fast and precise determination of short and medium sized DNA fragments
- Sharp bands are obtained after electrophoresis
- Suitable for gels stained with ethidium bromide, SYBR Green or other stain for DNA visualization
- Ready-to-use: direct gel loading
DNA fragment lengths (bp):
1500, 800, 400, 200, 50
M50-1500 Fast DNA Ladder ready-to-use (MR27) - To be discontinued - Manual
Usage
- For the best results, apply approx. 10 μl of the DNA ladder per well.
- Mix 1 volume of DNA loading dye with 5 volumes of analyzed DNA before application
6x FastLadder Loading Dye
10 mM Tris-HCl (pH 7.6), 60 mM EDTA, 0.03% bromophenol blue and 60% glycerol
Storage conditions
Keep at +4°C or room temperature for short-term or at -20°C for long-term storage.
Quality control
The DNA ladders have been tested in various kinds of agarose and polyacrylamide gels. The absence of nucleases has been confirmed by the relevant QC procedures.