54,00 € – 454,00 €
M100-1000 DNA Ladderis a molecular weight standard specially designed for electrophoretic analysis of small and medium DNA fragments on agarose and non-denaturing polyacrylamide gels. Ideal for sizing of PCR products and relatively small double-stranded DNA. It is especially useful for determining 100 bp differences between DNA fragments. M100-1000 DNA ladder contain purified blunt-ended PCR products.
DNA Ladders are supplied with 6x Green DNA Loading Dye (AG18) (500 µl).
The M100-1000 ready-to-use molecular weight DNA ladder is designed for determining the size of short and medium PCR products and dsDNA fragments in the 100 – 1000 bp range. The M100-1000 DNA Ladder is a mixture of specially selected, purified blunt-ended PCR products. It gives a pattern of regularly spaced ten bands, ranging from 100 bp and 1000 bp, differing in size by 100 bp. A DNA loading buffer (6x GREEN) is provided with each DNA ladder. The loading buffer contains Ficoll® 400, which facilitates rapid sinking to the bottom of the well in the agarose or polyacrylamide gel. The solution protects DNA thanks to the presence of EDTA, which inactivates nucleases and other enzymes by binding the divalent metal ions crucial to their activity. The 6x GREEN solution contains two different dyes which migrate in the electrophoretic field differently from the standard size PCR products, ensuring that they do not hinder the analysis by image obscuration. The orange G dye corresponds to 50 bp and xylene cyanol FF to 4000 bp PCR
products in a 1% agarose gel.
The M100-1000 DNA Ladder is also supplied in a M100-1000 ready-to-use version pre-mixed with 6x GREEN DNA loading buffer.
Features and advantages
- Allows precise determination of short and medium sized DNA fragments
- Band sizes are easily remembered (differ by 100 bp)
- Sharp bands are obtained after electrophoresis
- Suitable for gels stained with ethidium bromide, SYBR Green or other stain for DNA visualization
- Ready-to-use: direct gel loading
DNA fragment lengths (bp):
1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
M100-1000 DNA Ladder (MR61) - Manual
- For the best results, apply approx. 10 μl of the DNA ladder per well.
- Mix 1 volume of DNA loading dye with 5 volumes of analyzed DNA and/or DNA ladder before application.
10 mM Tris-HCl (pH 7.6), 1 mM EDTA
6x GREEN DNA Loading Buffer
20 mM Tris-HCl (pH 7.6), 60 mM EDTA, 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400
Keep at +4°C or room temperature for short-term or at -20°C for long-term storage.
The DNA ladders have been tested in various kinds of agarose and polyacrylamide gels. The absence of nucleases has been confirmed by the relevant QC procedures.
M100-1000 DNA Ladder (MR61) - MSDS
M100-1000 DNA Ladder (MR61) - CoA 1.2017 L.725872
M100-1000 DNA Ladder (MR61) - CoA 1.2018 L.626772
Post-mortem detection of six human herpesviruses (HSV-1, HSV-2, VZV, EBV, CMV, HHV-6) in trigeminal and facial nerve ganglia by PCR
Authors: Iwona Ptaszyńska-Sarosiek, Justyna Dunaj, Agata Zajkowska, Anna Niemcunowicz-Janica, Monika Król, Sławomir Pancewicz, Joanna Zajkowska Products: M100-1000 DNA Ladder (MR61), M100-500 DNA Ladder (MR71) Year: 2019 Source: PeerJ, 2019 January, Vol. 9
Campylobacter spp. and bacteriophages from broiler chickens: Characterization of antibiotic susceptibility profiles and lytic bacteriophages
Authors: Anna Nowaczek, Renata Urban‐Chmiel, Marta Dec, Andrzej Puchalski, Dagmara Stępień‐Pyśniak, Agnieszka Marek, Ewelina Pyzik Products: M100-1000 DNA Ladder (MR61), M100-3000 DNA Ladder (MR17) Year: 2019 Source: Microbiology Open
Influence of Pasteurella multocida Toxin on the differentiation of dendritic cells into osteoclasts
Authors: Chakraborty S., Kloos B., Roetz N., Schmidt S., Eigenbrod T., Kamitani S., Kubatzky K.F. Products: EXTRACTME TOTAL RNA KIT (EM09) Year: 2018 Source: Elsevier, Immunobiology
Detection of BHV-1 and BRSV viruses in European bison in the Białowieża forest: a preliminary study
Authors: Renata Urban-Chmiel, Andrzej Wernicki, Diana Stęgierska, Jan Marczuk, Jerzy Rola, Wojciech Socha & Jose L. Valverde Piedra Products: M100-1000bp DNA marker Year: 2017 Source: Journal of Applied Animal Research
A new assay for the simultaneous identification and differentiation of Klebsiella oxytoca strains
Authors: Karolina Stojowska-Swędrzyńska, Beata Krawczyk Products: M100-1000bp DNA marker, Taq DNA polymerase Year: 2016 Source: Appl Microbiol Biotechnol (2016)
Modified 16S–23S rRNA intergenic region restriction endonuclease analysis for species identification of Enterococcus strains isolated from pigs, compared with identification using classical methods and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Authors: Aneta Nowakiewicz, Grażyna Ziółkowska, Przemysław Zięba, Aleksandra Trościańczyk, Tomasz Banach and Cezary Kowalski Products: M100-1000bp DNA marker Year: 2015 Source: Journal of Medical Microbiology (2015)
Identification of Lactobacillus strains of goose origin using MALDI-TOF mass spectrometry and 16Se23S rDNA intergenic spacer PCR analysis
Authors: Marta Dec, Renata Urban-Chmiel, Sebastian Gnat, Andrzej Puchalski, Andrzej Wernicki Products: M100-1000 DNA Ladder, dNTPs, Pwo Polymerase, Primers Year: 2014 Source: Elsevier
Phenotypic and Genotypic diversity of Pseudomonas aeruginosa strains isolated from hospitals in Siedlce(Poland)
Authors: Wolska Katarzyna, Barbara Kot, Antoni Jakubczak Products: M100-1000 DNA Ladder, Taq DNA polymerase Year: 2012 Source: Brazilian Journal of Microbiology
BOX-PCR is an adequate tool for typing of clinical Pseudomonas aeruginosa isolates
Authors: Wolska Katarzyna, Barbara Kot, Rymuza Katarzyna Products: M100-1000 DNA Ladder, Taq DNA polymerase, PCR buffer Year: 2011 Source: ResearchGate