M100-1000 -DNA Ladder ready-to-use is a molecular weight standard specially designed for electrophoretic analysis of small and medium DNA fragments on agarose and non-denaturing polyacrylamide gels. Ideal for sizing of PCR products and relatively small double-stranded DNA. It is especially useful for determining 100 bp differences between DNA fragments. M100-1000 DNA ladder contain purified blunt-ended PCR products.
DNA Ladders are supplied with 6x Green DNA Loading Dye (AG18) (500 µl).
The M100-1000 ready-to-use molecular weight DNA ladder is designed for determining the size of short and medium PCR products and dsDNA fragments in the 100 – 1000 bp range. The M100-1000 DNA Ladder is a mixture of specially selected, purified blunt-ended PCR products. It gives a pattern of regularly spaced ten bands, ranging from 100 bp and 1000 bp, differing in size by 100 bp. A DNA loading buffer (6x GREEN) is provided with each DNA ladder. The loading buffer contains Ficoll® 400, which facilitates rapid sinking to the bottom of the well in the agarose or polyacrylamide gel. The solution protects DNA thanks to the presence of EDTA, which inactivates nucleases and other enzymes by binding the divalent metal ions crucial to their activity. The 6x GREEN solution contains two different dyes which migrate in the electrophoretic field differently from the standard size PCR products, ensuring that they do not hinder the analysis by image obscuration. The orange G dye corresponds to 50 bp and xylene cyanol FF to 4000 bp PCR
products in a 1% agarose gel.
The M100-1000 DNA Ladder is also supplied in a M100-1000 ready-to-use version pre-mixed with 6x GREEN DNA loading buffer.
Features and advantages
- Allows precise determination of short and medium sized DNA fragments
- Band sizes are easily remembered (differ by 100 bp)
- Sharp bands are obtained after electrophoresis
- Suitable for gels stained with ethidium bromide, SYBR Green or other stain for DNA visualization
- Ready-to-use: direct gel loading
DNA fragment lengths (bp):
1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
M100-1000 DNA Ladder (MR61) – Discontinued - Manual
- For the best results, apply approx. 10 μl of the DNA ladder per well.
- Mix 1 volume of DNA loading dye with 5 volumes of analyzed DNA
and/or DNA ladder before application.
10 mM Tris-HCl (pH 7.6), 1 mM EDTA
6x GREEN DNA Loading Buffer
20 mM Tris-HCl (pH 7.6), 60 mM EDTA, 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400
Keep at +4°C or room temperature for short-term or at -20°C for long-term storage.
The DNA ladders have been tested in various kinds of agarose and polyacrylamide gels. The absence of nucleases has been confirmed by the relevant QC procedures.