181,00 €The LM Agarose Low Melting Point is ideal for preparative electrophoresis and the recovery of DNA and RNA. It is characterised by low melting and gelling point. When working with the LM Agarose Low Melting Point, there is no need for extracting the DNA from a gel before manipulations such as ligation, transformation, restriction, amplification and sequencing. It is useful for the separation of nucleic acids in the range of 100 – 23 000 bp.
Features and advantages
- Low melting and gelling point
- High clarity and low background
- DNase and RNase free
- High purity (MolecularBiology Grade)
- Conventional and preparative electrophoresis of nucleic acids in a 100 – 23 000 bp range
- Recovery of high quality RNA and DNA after electrophoretic separation
- Direct enzymatic manipulations in the dissolved agarose gel
Store at room temperature
Shipping at ambient temperature
LM Agarose (AG43) - Manual
Dissolve the appropriate quantity of agarose in 1x TAE/ 1x TBE buffer by heating the suspension in a microwave or water bath. Use caution when handling as the temperature of the solution may be extremely high. Cool the solution to approx. 60°C before pouring.
The detection of nucleic acids in agarose gels can be carried out with ethidium bromide or other commercially available stains for DNA visualization.
For obtaining the best separation of DNA fragments, the following recommendations should be applied.
LM Agarose (AG43) - MSDS
LM Agarose (AG43) - CoA 1.2018 L.795673
LM Agarose (AG43) - CoA 1.2018 L.736572
LM Agarose (AG43) - CoA 1.2018 L.836772
Antioxidant and DNA Repair Stimulating Effect of Extracts from Transformed and Normal Roots of Rhaponticum carthamoides against Induced Oxidative Stress and DNA Damage in CHO Cells
Authors: Ewa Skała, Przemysław Sitarek, Marek Różalski, Urszula Krajewska, Janusz Szemraj, Halina Wysokińska, and Tomasz Śliwiński Products: LM Agarose (AG43), EXTRACTME TOTAL RNA KIT (EM09), TaqNova DNA Polymerase (RP702) Year: 2016 Source: Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2016