IDEAL II DNA Ladder is a olecularm weight standard specially designed for electrophoretic analysis of medium and large DNA fragments on agarose and non-denaturing polyacrylamide gels. Ideal for sizing of PCR products, digested plasmids and medium or large double-stranded DNA fragments.
IDEAL II DNA ladder contain purified blunt-ended PCR products and cohesive-ended restriction fragments.
Ready-to-use version is pre-mixed with 6x GREEN DNA loading buffer.
DNA Ladders are supplied with 6x Green DNA Loading Dye (500 µl).
The IDEAL II molecular weight DNA ladder is designed for determining the size of large and medium PCR products, digested plasmids and other dsDNA fragments in the 700 – 9276 bp range. The IDEAL II DNA Ladder is a mixture of specially selected, purified, blunt-ended PCR products and sticky-ended DNA restriction products.
It gives a clear band pattern. A DNA loading buffer (6x GREEN) is provided with each DNA ladder. The loading buffer contains Ficoll® 400, which facilitates rapid sinking to the bottom of the well in the agarose or polyacrylamide gel.
The solution protects DNA thanks to the presence of EDTA, which inactivates nucleases and other enzymes by binding the divalent metal ions crucial to their activity.
The 6xGREEN solution contains two different dyes which migrate in the electrophoretic field differently from the standard size PCR products, ensuring that they do not hinder the analysis by image obscuration. The orange G dye corresponds to 50 bp and xylene cyanol FF to 4000 bp PCR products in a 1% agarose gel.
The IDEAL II DNA Ladder is also supplied in a ready-to-use version pre-mixed with 6x GREEN DNA loading buffer.
Features and advantages
- Allows precise determination of large and medium sized DNA fragments
- Gives a clear band pattern in a wide DNA size range
- Sharp bands are obtained after electrophoresis
- Suitable for gels stained with ethidium bromide, SYBR Green or other stain for DNA visualization
- Ready-to-use: direct gel loading
DNA fragment lengths (bp):
9276, 5982, 3906, 2688, 2000, 1500, 1000, 700
IDEAL II DNA Ladder ready-to-use (MR25) - Manual
- For the best results, apply approx. 10 μl of the DNA ladder per well.
- Mix 1 volume of DNA loading dye with 5 volumes of analyzed DNA and/or DNA ladder before application.
6x GREEN DNA Loading Buffer
20 mM Tris-HCl (pH 7.6), 60 mM EDTA, 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400
Keep at +4°C or room temperature for short-term or at -20°C for long-term storage.
The DNA ladders have been tested in various kinds of agarose and polyacrylamide gels. The absence of nucleases has been confirmed by the relevant QC procedures.