The Hypernova DNA Polymerase is a recombinant, thermostable and proofreading Pwo DNA polymerase, originally isolated from the hyperthermophilic archaeon Pyrococcus woesei. The enzyme can generate very long amplicons (up to 10 kbp).
The polymerase is recommended for the multiplex PCR as it works well in a wide range of Mg2+, salt concentration and pH. It is also recommended for the amplification of difficult templates (regions abundant in GC, palindromes and multiple repeats).
Available Options:
| SKU | Unit | ||
|---|---|---|---|
 | RP235 | 1000 U ( 2U/μl ) | Ask for an offer |
 | RP232 | 200 U ( 2U/μl ) | Ask for an offer |
The Hypernova DNA Polymerase is a recombinant, thermostable and proofreading Pwo DNA polymerase, originally isolated from the hyperthermophilic archaeon Pyrococcus woesei. The enzyme can generate very long amplicons (up to 10 kbp).
Hypernova is a versatile and easy-to-use polymerase, since it works with many different protocols and requires minimal time consuming optimization.
The Hypernova polymerase catalyses a DNA replication reaction at 72°C. The halftime of the polymerase at 95°C is over 8 hours. It has 3’ → 5’ exonuclease activity (proofreading activity). No 5’ → 3’ exonuclease activity increases stability of the PCR products. It leaves blunt-ended 3’ endings (important in molecular cloning).
The polymerase is recommended for the multiplex PCR as it works well in a wide range of Mg2+, salt concentration and pH. It is also recommended for the amplification of difficult templates (regions abundant in GC, palindromes and multiple repeats).
Features and advantages
- Increased processivity (for long amplicons, up to 10 kbp)
- High yield with minimal amounts of enzyme and little optimization
- High fidelity (proofreading activity)
- Ideal for difficult templates which fail with standard Taq DNA polymerases
- Fool proof during multiplex PCR
- Very specific and sensitive
- More thermostable than Taq polymerase
Applications
- Reproducible amplification of long templates
- Multiplex PCR
- Cloning, site-directed mutagenesis and other methods, which require high fidelity
- Amplification of regions abundant in GC
Unit definition
One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 72°C in a 50 μl reaction.
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Additional information
Both reaction buffers provided may be used with Hypernova DNA polymerase. 10 x Hypernova buffer is recommended as first approach and for applications requiring high specificity. 10 x Shark buffer is recommended for applications where high sensitivity and amplification efficiency is required (e.g. for amplification of multiple products). Both buffers may be evaluated to determine the buffer most suitable for specific application.
Storage buffer
20 mM Tris-HCl (pH 7.4, 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% (v/v) glycerol
Reaction buffers
10 x Hypernova
100 mM Tris-HCl (pH 8.3, 25°C), 500 mM KCl, 1.5% Triton X-100
10 x Shark
200 mM Tris-HCl (pH 8.3, 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 1.0% Triton X-100
Storage conditions
Store all components at -20°C.
Shipping conditions
Shipping on dry or blue ice.
Quality control
Extensively tested in various PCR reactions. Free of unspecific nucleases.
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