The Hypernova DNA Polymerase is a recombinant, thermostable and proofreading Pwo DNA polymerase, originally isolated from the hyperthermophilic archaeon Pyrococcus woesei. The enzyme can generate very long amplicons (up to 10 kbp).
Hypernova is a versatile and easy-to-use polymerase, since it works with many different protocols and requires minimal time consuming optimization.

The Hypernova polymerase catalyses a DNA replication reaction at 72°C. The halftime of the polymerase at 95°C is over 8 hours. It has 3’ → 5’ exonuclease activity (proofreading activity). No 5’ → 3’ exonuclease activity increases stability of the PCR products. It leaves blunt-ended 3’ endings (important in molecular cloning).

The polymerase is recommended for the multiplex PCR as it works well in a wide range of Mg²⁺, salt concentration and pH. It is also recommended for the amplification of difficult templates (regions abundant in GC, palindromes and multiple repeats).

Features and advantages

• Increased processivity (for long amplicons, up to 10 kbp)
• High yield with minimal amounts of enzyme and little optimization
• High fidelity (proofreading activity)
• Ideal for difficult templates which fail with standard Taq DNA polymerases
• Fool proof during multiplex PCR
• Very specific and sensitive
• More thermostable than Taq polymerase

Applications

• Reproducible amplification of long templates
• Multiplex PCR
• Cloning, site-directed mutagenesis and other methods, which require high fidelity
• Amplification of regions abundant in GC

Unit definition

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 72°C in a 50 μl reaction.