125,00 € – 217,00 €
EXTRAzol is a ready-to-use reagent for the isolation of separate fractions of RNA, DNA and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.
- Additional information
- Product information
- Protocol & Manual
- Technical Tips
- Quality & Safety
100 ml, 200 ml
EXTRAzol is a ready-to-use reagent for the isolation of total RNA from various biological materials such as animal and tissues (rich in polysaccharides andproteoglycans), cell culture and bacterial cells.
Using EXTRAzol a biological sample is homogenized or lysed before being separated into three phases: an aqueous phase (upper), an organic phase (lower) and an interphase. The RNA remains in the aqueous phase and its purification is followed by precipitation in isopropyl alcohol. The highly effective RNase inhibitory property of EXTRAzol protects the integrity of the RNA during lysis and results in the isolation of high-quality material (high RIN and S28/S18 values). EXTRAzol contains phenol and the mixture of other reagents to ensure optimal results.
1 ml of EXTRAzol is sufficient to isolate total RNA from 1x 107 cells or 100 mg of tissue. The isolation method is fast and easy to carry.
RNA isolation obtained by using EXTRAzol provides reliable results in the gene expression based studies using such applications as synthesis of cDNA, real-time RT-PCR, microarrays, hybridization assays, in vitro translation.
Protocol & Manual
EXTRAzol (EM30) - Manual
Store at +4°C
Shipping at room temperature
Quality & Safety
Each LOT of the EXTRAzol reagent is tested in accordance to internal procedures.