EXTRAzol is a ready-to-use reagent for the isolation of separate fractions of RNA, DNA and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.
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EXTRAzol is a ready-to-use reagent for the isolation of total RNA from various biological materials such as animal and tissues (rich in polysaccharides andproteoglycans), cell culture and bacterial cells.
Using EXTRAzol a biological sample is homogenized or lysed before being separated into three phases: an aqueous phase (upper), an organic phase (lower) and an interphase. The RNA remains in the aqueous phase and its purification is followed by precipitation in isopropyl alcohol. The highly effective RNase inhibitory property of EXTRAzol protects the integrity of the RNA during lysis and results in the isolation of high-quality material (high RIN and S28/S18 values). EXTRAzol contains phenol and the mixture of other reagents to ensure optimal results.
1 ml of EXTRAzol is sufficient to isolate total RNA from 1x 107 cells or 100 mg of tissue. The isolation method is fast and easy to carry.
RNA isolation obtained by using EXTRAzol provides reliable results in the gene expression based studies using such applications as synthesis of cDNA, real-time RT-PCR, microarrays, hybridization assays, in vitro translation.
EXTRAzol (EM30) - Manual
Store at +4°C
Shipping at room temperature
Each LOT of the EXTRAzol reagent is tested in accordance to internal procedures.
EXTRAzol (EM30) - MSDS
EXTRAzol (EM30) - CoA 2.2017 L.766672
EXTRAzol (EM30) - CoA 1.2017 L.846072
S1P-lyase deficiency uncouples ganglioside formation – Potential contribution to tumorigenic capacity
Authors Nadine Hagen-Euteneuer, Shah Alam, Hannah Rindsfuesser, Dagmar Meyer zu Heringdorf, Gerhild van Echten-Deckert Products EXTRAzol (EM30) Year 2020 Source Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
SATB1 as oncogenic driver and potential therapeutic target in head & neck squamous cell carcinoma (HNSCC)
Authors Omkar Panchal, Gunnar Wichmann, Reidar Grenman, Lisa Eckhardt, Leoni A. Kunz-Schughart, Heike Franke, Andreas Dietz, Achim Aigner Products EXTRAzol (EM30) Year 2020 Source Scientific Reports 10, 8615
Neurodegeneration Caused by S1P-Lyase Deficiency Involves Calcium-Dependent Tau Pathology and Abnormal Histone Acetylation
Authors Shah Alam, Antonia Piazzesi, Mariam Abd El Fatah, Maren Raucamp and Gerhild van Echten-Deckert, Products EXTRAzol (EM30) Year 2020 Source Cells 2020, 9(10), 2189
SARS-CoV-2: Comparative analysis of different RNA extraction methods
Authors Cecilia Ambrosi, Carla Prezioso, Paola Checconi, Daniela Scribano, Meysam Sarshar, Maurizio Capannari, Carlo Tomino, Massimo Fini, Enrico Garaci, Anna Teresa Palamara, Giovanna De Chiara, Dolores Limongi Products EXTRAzol (EM30) Year 2020 Source Journal of Virological Methods, Volume 287, January 2021, 114008
The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro
Authors Smieszek, A.; Marcinkowska, K.; Pielok, A.; Sikora, M.; Valihrach, L.; Marycz, K. Products EXTRAzol (EM30) Year 2020 Source Cells, , 9, 479
Effect of Anthocyanin-Rich Extract of Sour Cherry for Hyperglycemia-Induced Inflammatory Response and Impaired Endothelium-Dependent Vasodilation
Authors Markovics A., Biró A., Kun-Nemes A., Fazekas MÉ., Rácz AA., Paholcsek M., Lukács J., Stündl L., Remenyik J. Products EXTRAzol (EM30) Year 2020 Source Nutrients. 2020; 12(11):3373
Theranostic applications of nanostructured silicate-substituted hydroxyapatite co-doped with Eu3+ and Bi3+ ions-novel strategy for bone regeneration
Authors Sara Targonska, Mateusz Sikora, Krzysztof Marycz, Agnieszka Smieszek, and Rafal J. Wiglusz Products EXTRAzol (EM30) Year 2020 Source ACS Biomaterials Science & Engineering
Zirconium Oxide Thin Films Obtained by Atomic Layer Deposition Technology Abolish the Anti-Osteogenic Effect Resulting from miR-21 Inhibition in the Pre-Osteoblastic MC3T3 Cell Line
Authors Seweryn A, Pielok A, Lawniczak-Jablonska K, Pietruszka R, Marcinkowska K, Sikora M, Witkowski BS, Godlewski M, Marycz K, Smieszek A Products EXTRAzol (EM30) Year 2020 Source International journal of nanomedicine vol. 15 1595-1610
Evaluation of the presence of ToLCNDV and CGMMV viruses in commercial seeds of cucurbits
Authors García Pérez Arcadio Products EXTRAzol (EM30) Year 2019 Source http://hdl.handle.net/10251/123830
Evaluación de la presencia de los virus ToLCNDV y CGMMV en semillas comerciales de cucurbitáceas
Authors Arcadio García Pérez Products EXTRAzol (EM30) Year 2019 Source riunet.upv.es
Expression of odorant‐binding proteins in mouthpart palps of the desert locust Schistocerca gregaria
Authors P. Pregitzer, M. Zielonka, A.‐S. Eichhorn, X. Jiang, J. Krieger, H. Breer Products EXTRAzol (EM30) Year 2018 Source Insect Mol Biol, 28: 264-276