The EXTRACTME TOTAL RNA KIT is designed for a rapid and efficient purification of high quality RNA from up to 30 mg of tissue (fresh or frozen) and up to 107 cultured cells. The isolation protocols and buffer formulations were optimized for high isolation efficiency and purity of RNA. The product is intended for research use only.
Available Options:
SKU | Exctractions | ||
---|---|---|---|
![]() | EM09.1-250 | 250 extractions | Ask for an offer |
![]() | EM09.1-050 | 50 extractions | Ask for an offer |
PRINCIPLE
The EXTRACTME TOTAL RNA KIT utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. During the first isolation step, a tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). A homogenate is lysed with guanidine thiocyanate and detergents. RNases are inactivated by guanidine thiocyanate and ß-mercaptoethanol (optional). The homogenate is separated from undigested tissue/cell that remains after centrifugation. RNA bounds to a Purification Column membrane by addition of ethanol. On-column DNase digestion enables removal of remaining genomic DNA. A three-step washing stage effectively removes impurities and enzyme inhibitors. Purified RNA is eluted with the use of low ionic strength buffer or RNase-free water and may be used directly in all downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RNA sequencing, microarrays.
PRODUCT SPECIFICATIONS
SAMPLE MATERIAL
- fresh or frozen tissue : up to 30 mg
- tissue preserved in RNase inactivating buffers (e.g. RNAlater®, Ambion): up to 30 mg
- cell culture: up to 107 cells
BINDING CAPACITY
~ 230 μg RNA
TIME REQUIRED
- 10–12 minutes (lysis and homogenization time not included)
- 15–30 minutes for homogenization in liquid nitrogen
- 15–20 minutes for mechanical homogenization (ceramic beads)
- 5 minutes for optional DNase I treatment
RNA PURITY
A260/A280 ratio = 1.9 – 2.1
EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - Manual
EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - Quick Protocol
EXTRACTME TOTAL RNA KIT – TROUBLESHOOTING
QUALITY CONTROL
The quality of each production batch (LOT) of the EXTRACTME TOTAL RNA KIT is tested with the use of standard QC procedures. Purified RNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.
EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - MSDS
EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - CoA 1.2018 L.755773
EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - CoA 1.2017 L.755773
EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - CoA 1.2017 L.626672
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Mechanistic insights into the synergistic activation of the RXR–PXR heterodimer by endocrine disruptor mixtures
Authors Vanessa Delfosse, Tiphaine Huet, Deborah Harrus, Meritxell Granell, Maxime Bourguet, Caroline Gardia-Parège, Barbara Chiavarina, Marina Grimaldi, Sébastien Le Mével, Pauline Blanc, David Huang, Jakub Gruszczyk, Barbara Demeneix, Sarah Cianférani, Jean-Baptiste Fini, Patrick Balaguer, and William Bourguet Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2021 Source Proceedings of the National Academy of Sciences Jan 2021, 118 (1) e2020551118 -
TGF-β Activity of a Demineralized Bone Matrix
Authors Layla Panahipour, Anes Omerbasic, Jila Nasirzade, Reinhard Gruber Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2021 Source Int. J. Mol. Sci. 2021, 22(2), 664 -
Micellar Casein and Whey Powder Hold a TGF-β Activity and Regulate ID Genes In Vitro
Authors Layla Panahipour, Selma Husejnovic, Jila Nasirzade, Stephan Semelmayer, Reinhard Gruber Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2021 Source Molecules 2021, 26(2), 507 -
Mechanistic insights into the synergistic activation of the RXR–PXR heterodimer by endocrine disruptor mixtures
Authors Delfosse V, Huet T, Harrus D, Granell M, Bourguet M, Gardia-Parège C, Chiavarina B, Grimaldi M, Le Mével S, Blanc P, Huang D, Gruszczyk J, Demeneix B, Cianférani S, Fini JB, Balaguer P, Bourguet W. Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2021 Source Proceedings of the National Academy of Sciences of the United States of America, 118(1), e2020551118 -
Milk lactoperoxidase decreases ID1 and ID3 expression in human oral squamous cell carcinoma cell lines
Authors Layla Panahipour, Maria De Biasi, Theresa Sophia Bokor, Alexandra Thajer, Nadja Haiden, Reinhard Gruber Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued, EXTRACTME TOTAL RNA KIT (EM09.2) – Discontinued Year 2020 Source Sci Rep 10, 5836 -
TGFβ activity released from platelet-rich fibrin adsorbs to titanium surface and collagen membranes
Authors Francesca Di Summa, Zahra Kargarpour, Jila Nasirzade, Alexandra Stähli, Goran Mitulović, Tanja Panić-Janković, Veronika Koller, Cosima Kaltenbach, Heinz Müller, Layla Panahipour, Reinhard Gruber, Franz-Josef Strauss Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued, EXTRACTME TOTAL RNA KIT (EM09.2) – Discontinued Year 2020 Source Scientific Reports 10 -
Platelet-Rich Fibrin Can Neutralize Hydrogen Peroxide-Induced Cell Death in Gingival Fibroblasts
Authors Zahra Kargarpour, Jila Nasirzade, Francesca Di Summa, Layla Panahipour, Richard J. Miron, Reinhard Gruber Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2020 Source Antioxidants, 9(6) -
SLy2‐deficiency promotes B‐1 cell immunity and triggers enhanced production of IgM and IgG2 antibodies against pneumococcal vaccine
Authors Jennifer Jaufmann, Leyla Tümen, Fee Schmitt, Daniel Schäll, Max von Holleben, Sandra Beer‐Hammer Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued, TRANSCRIPTME RNA Kit (RT31) Year 2020 Source Immun Inflamm Dis. 2020; 1– 17 -
Hypoallergenic infant formula lacks transforming growth factor beta activity and has a lower anti-inflammatory activity than regular infant formula
Authors Layla Panahipour, Amirali Abooneghab Tabatabaei, Reinhard Gruber Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2020 Source Journal of Dairy Science -
Butyrate Decreases ICAM-1 Expression in Human Oral Squamous Cell Carcinoma Cells
Authors Gabriel Leonardo Magrin, Francesca Di Summa, Franz-Josef Strauss, Layla Panahipour, Michael Mildner, Cesar Augusto Magalhães Benfatti, Reinhard Gruber Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2020 Source Int. J. Mol. Sci. 2020, 21(5), 1679; -
Dual Inhibitory Action of a Novel AKR1C3 Inhibitor on Both Full-Length AR and the Variant AR-V7 in Enzalutamide Resistant Metastatic Castration Resistant Prostate Cancer
Authors Mona Kafka, Fabian Mayr, Veronika Temml, Gabriele Möller, Jerzy Adamski, Julia Höfer, Stefan Schwaiger, Isabel Heidegger, Barbara Matuszczak, Daniela Schuster, Helmut Klocker, Jasmin Bektic, Hermann Stuppner, Iris E. Eder Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2020 Source Cancers 12, 2092 -
Administration of silver nanoparticles affects ovarian steroidogenesis and may influence thyroid hormone metabolism in hens (Gallus domesticus)
Authors Dorota Katarzyńska-Banasik, Małgorzata Grzesiak, Kinga Kowalik, Andrzej Sechman Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2020 Source Ecotoxicology and Environmental Safety, Volume 208 -
Effect of SUV39H1 Histone Methyltransferase Knockout on Expression of Differentiation-Associated Genes in HaCaT Keratinocytes
Authors Barbara Sobiak, Wiesława Leśniak Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2020 Source Cells 2020, 9(12), 2628 -
Biosynthesis of GlcNAc-rich N- and O-glycans in the Golgi apparatus does not require the nucleotide sugar transporter SLC35A3
Authors Bożena Szulc, Paulina Sosicka, Dorota Maszczak-Seneczko, Edyta Skurska, Auhen Shauchuk, Teresa Olczak, Hudson H. Freeze and Mariusz Olczak Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2020 Source Journal of Biological Chemistry(2020) 18:132 -
Platelet‐rich fibrin suppresses in vitro osteoclastogenesis
Authors Zahra Kargarpour, Jila Nasirzade, Franz Josef Strauss, Francesca Di Summa, Sadegh Hasannia, Heinz‐Dieter Müller, Reinhard Gruber Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source Journal of Periodontology, Volume 91, Issue 3 -
Inhibition of PIM1 blocks the autophagic flux to sensitize glioblastoma cells to ABT-737-induced apoptosis
Authors J. Remy, B. Linder, U. Weirauch, J. Konovalova, R. Marschalek, A. Aigner, D. Kögel Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1866, Issue 2, 2019, Pages 175-189 -
Prognostic Impact of Carboxylesterase 2 in Cholangiocarcinoma
Authors Benjamin Goeppert, Marcus Renner, Stephan Singer, Thomas Albrecht, Qiangnu Zhang, Arianeb Mehrabi, Anita Pathil, Christoph Springfeld, Bruno Köhler, Christian Rupp, Karl Heinz Weiss, Anja A. Kühl, Ruza Arsenic, Ulrich Frank Pape, Arndt Vogel, Peter Schirmacher, Stephanie Roessler & Nalân Utku Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source Scientific Reports, 2019, Vol 9, Article number: 4338 -
Milk modulates macrophage polarization in vitro
Authors Layla Panahipour, Evgeniya Kochergina, Alexandra Kreissl, Nadja Haiden, Reinhard Gruberab Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued, EXTRACTME TOTAL RNA KIT (EM09.2) – Discontinued Year 2019 Source Cytokine: X, Volume 1(2) -
Expression of the Sonic Hedgehog pathway components in clear cell renal cell carcinoma
Authors Anna Kotulak‑Chrzaszcz, Jakub Klacz, Marcin Matuszewski, Zbigniew Kmiec, Piotr M. Wierzbicki Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source Oncology Letters, 18, 5801-5810 -
Additive Pharmacological Interaction between Cisplatin (CDDP) and Histone Deacetylase Inhibitors (HDIs) in MDA-MB-231 Triple Negative Breast Cancer (TNBC) Cells with Altered Notch1 Activity—An Isobolographic Analysis
Authors Wawruszak, Anna and Luszczki, Jarogniew J. and Kalafut, Joanna and Okla, Karolina and Halasa, Marta and Rivero-Muller, Adolfo and Stepulak, Andrzej Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source Int. J. Mol. Sci. 2019, 20(15), 3663 -
Arsenic Trioxide and (−)-Gossypol Synergistically Target Glioma Stem-Like Cells via Inhibition of Hedgehog and Notch Signaling
Authors Linder, Benedikt and Wehle, Andrej and Hehlgans, Stephanie and Bonn, Florian and Dikic, Ivan and Rödel, Franz and Seifert, Volker and Kögel, Donat Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source Cancers 2019, 11(3), 350 -
Genetically Engineered Lung Cancer Cells for Analyzing Epithelial–Mesenchymal Transition
Authors Michał Kiełbus, Jakub Czapiński, Joanna Kałafut, Justyna Woś, Andrzej Stepulak, Adolfo Rivero-Müller Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source Cells 2019, 8(12), 1644; -
S100A6 activates EGFR and its downstream signaling in HaCaT keratinocytes
Authors Agnieszka Graczyk‐Jarzynka, Barbara Sobiak, Michał Mlącki, Tomasz Wilanowski, Wiesława Leśniak Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source J Cell Physiol. 2019; 234: 17561– 17569 -
Caffeic acid phenethyl ester protects against oxidative stress and dampens inflammation via heme oxygenase 1
Authors Alexandra Stähli, Ceeneena Ubaidha Maheen, Franz Josef Strauss, Sigrun Eick, Anton Sculean & Reinhard Gruber Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source International Journal of Oral Science, 2019, Vol 11, Article number: 6 -
Collagen I Promotes Adipocytogenesis in Adipose-Derived Stem Cells In Vitro
Authors Zöller N, Schreiner S, Petry L, Hoffmann S, Steinhorst K, Kleemann J, Jäger M, Kaufmann R, Meissner M, Kippenberger S. Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source Cells. 2019; 8(4):302. -
Selection of an inadequate housekeeping gene leads to misinterpretation of target gene expression in zinc deficiency and zinc supplementation models
Authors Veronika Kloubert, Lothar Rink Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2019 Source Journal of Trace Elements in Medicine and Biology, Volume 56, Pages 192-197 -
The anti-inflammatory effect of milk and dairy products on periodontal cells: an in vitro approach
Authors Layla Panahipour, Shayesteh Nasserzare, Zeinab Amer, Friedrich Brücke, Alexandra Stähli, Alexandra Kreissl, Nadja Haiden, Reinhard Gruber Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued, EXTRACTME TOTAL RNA KIT (EM09.2) – Discontinued Year 2018 Source Clin Oral Invest (2019) 23: 1959 -
Influence of Pasteurella multocida Toxin on the differentiation of dendritic cells into osteoclasts
Authors Chakraborty S., Kloos B., Roetz N., Schmidt S., Eigenbrod T., Kamitani S., Kubatzky K.F. Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2018 Source Elsevier, Immunobiology -
Doxycycline Impairs Mitochondrial Function and Protects Human Glioma Cells from Hypoxia-Induced Cell Death: Implications of Using Tet-Inducible Systems
Authors Anna-Luisa Luger, Benedikt Sauer ID , Nadja I. Lorenz, Anna L. Engel, Yannick Braun, Martin Voss, Patrick N. Harter, Joachim P. Steinbach and Michael W.Ronellenfitsch Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2018 Source Int. J. Mol. Sci. 2018, 19, 1504; doi:10.3390/ijms19051504 -
Nobiletin acts anti-inflammatory on murine IL-10−/− colitis and human intestinal fibroblasts
Authors Yvonne Hagenlocher, Sonja Gommeringer, Anna Held, Katharina Feilhauer, Jörg Köninger, Stephan C. Bischoff, Axel Lorentz Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2018 Source European Journal of Nutrition -
Impact of OGT deregulation on EZH2 target genes FOXA1 and FOXC1 expression in breast cancer cells
Authors Ewa Forma, Paweł Jóźwiak, Piotr Ciesielski, Agnieszka Zaczek, Katarzyna Starska, Magdalena Bryś, Anna Krześlak Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2018 Source PLOS ONE -
Diverse Regulation of Vitamin D Receptor Gene Expression by 1,25-Dihydroxyvitamin D and ATRA in Murine and Human Blood Cells at Early Stages of Their Differentiation
Authors Sylwia Janik, Urszula Nowak, Agnieszka Łaszkiewicz, Anastasia Satyr, Michał Majkowski, Aleksandra Marchwicka, Łukasz Snieżewski, Klaudia Berkowska, Marian Gabryś, Małgorzata Cebrat and Ewa Marcinkowska Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2017 Source Int. J. Mol. Sci. 2017, 18, 1323; doi:10.3390/ijms18061323 International Journal of Molecular Sciences -
Attenuation of H2O2-induced neuronal cell damage by piceatannol
Authors Joanna Gerszon, Anna Walczak, Aleksandra Rodacka Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2017 Source Elsevier -
Overexpression of the YAP1 oncogene in clear cell renal cell carcinoma is associated with poor outcome
Authors Agnieszka Rybarczyk, Jakub Klacz, Agata Wronska, Marcin Mat uszewski, Zbigniew Kmiec and Piotr M. Wierzbicki Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2017 Source ONCOLOGY REPORTS 38: 427-439, 2017 -
The Effect of Leonurus sibiricus Plant Extracts on Stimulating Repair and Protective Activity against Oxidative DNA Damage in CHO Cells and Content of Phenolic Compounds
Authors Przemysław Sitarek, Ewa Skała, Halina Wysokińska, Marzena Wielanek, Janusz Szemraj, Monika Toma, and Tomasz Śliwiński Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued, TaqNova DNA Polymerase (RP7A) Year 2016 Source Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2016, Article ID 5738193, 11 pages -
All-Trans Retinoic Acid Modulates DNA Damage Response and the Expression of the VEGF-A and MKI67 Genes in ARPE-19 Cells Subjected to Oxidative Stress
Authors Paulina Tokarz, AgnieszkaWanda Piastowska-Ciesielska, Kai Kaarniranta and Janusz Blasiak Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2016 Source Int. J. Mol. Sci. 2016, 17, 898; doi:10.3390/ijms17060898 International Journal of Molecular Sciences -
Antioxidant and DNA Repair Stimulating Effect of Extracts from Transformed and Normal Roots of Rhaponticum carthamoides against Induced Oxidative Stress and DNA Damage in CHO Cells
Authors Ewa Skała, Przemysław Sitarek, Marek Różalski, Urszula Krajewska, Janusz Szemraj, Halina Wysokińska, and Tomasz Śliwiński Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued, LM Agarose (AG43) – Discontinued, TaqNova DNA Polymerase (RP7A) Year 2016 Source Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2016 -
Decreased expression of RASSF1A tumor suppressor gene is associated with worse prognosis in clear cell renal cell carcinoma
Authors Jakub Klacz, Piotr M. Wierzbicki, Agata Wronska, Agnieszka Rybarczyk, Marcin Stanislawowski, Tomasz Slebiod, Agata Olejniczak, Marcin Matuszewski and Zbigniew Kmiec Products EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued Year 2015 Source INTERNATIONAL JOURNAL OF ONCOLOGY 48: 55-66, 2016