EXTRACTME TOTAL RNA KIT (EM09.1) – Discontinued

The EXTRACTME TOTAL RNA KIT is designed for a rapid and efficient purification of high quality RNA from up to 30 mg of tissue (fresh or frozen) and up to 107 cultured cells. The isolation protocols and buffer formulations were optimized for high isolation efficiency and purity of RNA. The product is intended for research use only.

EM09.1, EM09.1-010, EM09.1-050, EM09.1-250

Available Options:
SKUExctractions
EM09.1-250250 extractionsAsk for an offer
EM09.1-05050 extractionsAsk for an offer

PRINCIPLE

The EXTRACTME TOTAL RNA KIT utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. During the first isolation step, a tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). A homogenate is lysed with guanidine thiocyanate and detergents. RNases are inactivated by guanidine thiocyanate and ß-mercaptoethanol (optional). The homogenate is separated from undigested tissue/cell that remains after centrifugation. RNA bounds to a Purification Column membrane by addition  of ethanol. On-column DNase digestion enables removal of remaining genomic DNA. A three-step washing stage effectively removes impurities and enzyme inhibitors. Purified RNA is eluted with the use of low ionic strength buffer or RNase-free water and may be used directly in all downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RNA sequencing, microarrays.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL

  • fresh or frozen tissue : up to 30 mg
  • tissue preserved in RNase inactivating buffers (e.g. RNAlater®, Ambion): up to 30 mg
  • cell culture: up to 107 cells

BINDING CAPACITY

~ 230 μg RNA

TIME REQUIRED

  • 10–12 minutes (lysis and homogenization time not included)
  •  15–30 minutes for homogenization in liquid nitrogen
  • 15–20 minutes for mechanical homogenization (ceramic beads)
  • 5 minutes for optional DNase I treatment

RNA PURITY

A260/A280 ratio = 1.9 – 2.1

 

EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - Manual

EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - Quick Protocol

EXTRACTME TOTAL RNA KIT – TROUBLESHOOTING

QUALITY CONTROL

The quality of each production batch (LOT) of the EXTRACTME TOTAL RNA KIT is tested with the use of standard QC procedures. Purified RNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.

EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - MSDS


EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - CoA 1.2018 L.755773

EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - CoA 1.2017 L.755773

EXTRACTME TOTAL RNA KIT (EM09.1) - Discontinued - CoA 1.2017 L.626672