Available Options:
SKU | Exctractions | ||
---|---|---|---|
![]() | EM15-250 | 250 extractions | Ask for an offer |
![]() | EM15-050 | 50 extractions | Ask for an offer |
PRINCIPLE
The EXTRACTME RNA & DNA KIT utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. During the first isolation step, a tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). A homogenate is lysed with guanidine thiocyanate and detergents. Nucleases are inactivated by guanidine thiocyanate and β-mercaptoethanol (optional). The homogenate is separated from undigested tissue/cell that remains after centrifugation. First, DNA selectively bounds to DNA Purification Column. In the second order, RNA remaining in the filtrate bounds to an RNA Purification Column membrane in the presence of additional ethanol. A three-step washing stage effectively removes impurities and enzyme inhibitors. Purified RNA and DNA are eluted with the use of low ionic strength buffers and may be used directly in all downstream applications, such as PCR, qPCR, primer extension, sequencing, microarrays, Southern blotting, and in case of RNA also Northern blotting, RT-PCR, cDNA synthesis or stored until ready to use.
PRODUCT SPECIFICATIONS
SAMPLE MATERIAL
- fresh or frozen tissue: up to 30 mg
- tissue preserved in RNase inactivating buffers (e.g. RNAlater® , Ambion):
up to 30 mg - cell culture: up to 107 cells
BINDING CAPACITY
~230 μg RNA, ~60 μg DNA
TIME REQUIRED
- 10 minutes (for simultaneous RNA/DNA purification, lysis and homogenization time not included)
- 15–30 minutes for homogenization in liquid nitrogen
- 15–20 minutes for mechanical homogenization (ceramic beads)
RNA/DNA PURITY
A260/A280 ratio = 1.9 – 2.1
EXTRACTME RNA & DNA KIT (EM15) – Discontinued - Manual
EXTRACTME TOTAL RNA 96-WELL KIT- TROUBLESHOOTING
QUALITY CONTROL
The quality of each production batch (LOT) of the EXTRACTME RNA & DNA KIT is tested with the use of standard QC procedures. Purified RNA and DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.
EXTRACTME RNA & DNA KIT (EM15) – Discontinued - MSDS
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Co-Extraction Methods of DNA and total RNA for body fluid identification: a review
Authors Anh Tran Products EXTRACTME RNA & DNA KIT (EM15) – Discontinued Year 2020 Source Research repository of Murdoch University -
Evaluation of two DNA/RNA co-extraction methods for body fluid identification in forensics
Authors S. Loureiro, A. Amorim, L. Cainé, B. Silva, I. Gomes Products EXTRACTME RNA & DNA KIT (EM15) – Discontinued Year 2019 Source Forensic Science International: Genetics Supplement Series 1875-1768/ © 2019 Elsevier B.V. All rights reserved. Received 17 September 2019; Accepted 26 September 2019 -
Identification of the source of biological fluids recovered from samples collected at Sexual Assaultsscenarios using mRNA markers
Authors Sara Duarte Loureiro Products EXTRACTME RNA & DNA KIT (EM15) – Discontinued Year 2019 Source repositorio-aberto.up.pt -
IDENTIFICAÇÃO DA NATUREZA DE FLUÍDOS BIOLÓGICOS PROVENIENTES DE AMOSTRAS COLHIDAS NO ÂMBITO DE AGRESSÕES SEXUAIS ATRAVÉS DE mRNA
Authors Sara Loureiro, Iva Gomes, Benedita Silva, Laura Cainé Products EXTRACTME RNA & DNA KIT (EM15) – Discontinued Year 2019 Source In Livro de Resumos do IV Congresso Internacional da Associação Portuguesa de Ciências Forenses (Dinis-Oliveira RJ Ed). Dinis-Oliveira RJ, 159, Lisboa, 2019