EXTRACTME RNA BACTERIA & YEAST KIT (EM25)

EXTRACTME RNA BACTERIA & YEAST KIT (EM25)

38,00 613,00 

The EXTRACTME RNA BACTERIA & YEAST KIT is designed for a rapid and efficient purification of high quality RNA from broth yeast or bacteria cultures as well as frozen cells. The isolation protocol and buffer formulations were optimized for high isolation efficiency and purity of RNA. The product is intended for research use only.

EM25, EM25-010, EM25-050, EM25-250

Available Options:

PRINCIPLE

The EXTRACTME RNA BACTERIA & YEAST KIT utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. In the first step of the isolation procedure the cell walls are disintegrated and the membranes and proteins are degraded. Any RNases are inactivated by guanidine thiocyanate and ß-mercaptoethanol. The homogenate is separated from the  undigested cell remains by centrifugation and on the Homogenizing Column. The RNA is bound to  the Purification Column membrane by addition of ethanol. The three-step washing stage effectively removes impurities and enzyme inhibitors. Purified RNA is eluted with the use of a low ionic strength buffer or RNase-free water (pH 7.0–9.0) and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL

  • broth bacterial (up to 1 x 109 cells) or yeast (up to 5 x 107 cells) culture
  • frozen cell pellet

BINDING CAPACITY

approx. 90 μg RNA

YIELD

It depends on the sample material amount and type.

  • up to 60 μg RNA from bacterial cul
  • tureup to 30 μg RNA from yeast culture

TIME REQUIRED

  • 30 minutes for RNA isolation from bacteria (+ 10 minutes for optional DNase I treatment)
  • 50 minutes for RNA isolation from yeast (+ 10 minutes for optional DNase I treatment)

DNA PURITY

A260/A280 ratio = 1.9 – 2.1

RECOMMENDATIONS AND IMPORTANT NOTES

EXTRACTME RNA BACTERIA & YEAST KIT (EM25) - Manual

EXTRACTME RNA BACTERIA & YEAST KIT – TROUBLESHOOTING

QUALITY CONTROL

The quality of each production batch (LOT) of the EXTRACTME RNA BACTERIA & YEAST KIT is tested with the use of standard QC procedures. Purified RNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.

EXTRACTME RNA BACTERIA & YEAST KIT (EM25) - MSDS


EXTRACTME RNA BACTERIA & YEAST KIT (EM25) - CoA 2.2017 L.756572