PRINCIPLE

The EXTRACTME RNA BACTERIA & YEAST KIT utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. In the first step of the isolation procedure the cell walls are disintegrated and the membranes and proteins are degraded. Any RNases are inactivated by guanidine thiocyanate and ß-mercaptoethanol. The homogenate is separated from the  undigested cell remains by centrifugation and on the Homogenizing Column. The RNA is bound to  the Purification Column membrane by addition of ethanol. The three-step washing stage effectively removes impurities and enzyme inhibitors. Purified RNA is eluted with the use of a low ionic strength buffer or RNase-free water (pH 7.0–9.0) and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.