The EXTRACTME RNA BACTERIA & YEAST KIT is designed for a rapid and efficient purification of high quality RNA from broth yeast or bacteria cultures as well as frozen cells. The isolation protocol and buffer formulations were optimized for high isolation efficiency and purity of RNA. Purified RNA can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR. The product is intended for research use only.
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The EXTRACTME RNA BACTERIA & YEAST KIT utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. In the first step of the isolation procedure the cell walls are disintegrated and the membranes and proteins are degraded. Any RNases are inactivated by guanidine thiocyanate and ß-mercaptoethanol. The homogenate is separated from the undigested cell remains by centrifugation and on the Homogenizing Column. The RNA is bound to the Purification Column membrane by addition of ethanol. The three-step washing stage effectively removes impurities and enzyme inhibitors. Purified RNA is eluted with the use of a low ionic strength
buffer or RNase-free water (pH 7.0–9.0) and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.
- broth bacterial (up to 1 x 109 cells) or yeast (up to 5 x 10 7 cells) culture
- frozen cell pellet
approx. 90 μg RNA
It depends on the sample material amount and type.
- up to 60 μg RNA from bacterial cul
- tureup to 30 μg RNA from yeast culture
- 30 minutes for RNA isolation from bacteria (+ 10 minutes for optional DNase I treatment)
- 50 minutes for RNA isolation from yeast (+ 10 minutes for optional DNase I treatment)
A260/A280 ratio = 1.9 – 2.1
EXTRACTME RNA BACTERIA & YEAST KIT (EM25.1) - To be discontinued - Manual
EXTRACTME RNA BACTERIA & YEAST KIT – TROUBLESHOOTING
The quality of each production batch (LOT) of the EXTRACTME RNA BACTERIA & YEAST KIT is tested with the use of standard QC procedures. Purified RNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.
EXTRACTME RNA BACTERIA & YEAST KIT (EM25.1) - To be discontinued - MSDS
Unveiling the role of Gardnerella vaginalis in polymicrobial Bacterial Vaginosis biofilms: the impact of other vaginal pathogens living as neighbors
Authors Joana Castro, Daniela Machado & Nuno Cerca Products EXTRACTME RNA BACTERIA & YEAST KIT (EM25.1) – To be discontinued, EXTRACTME RNA BACTERIA & YEAST KIT (EM25) – Discontinued Year 2019 Source The ISME Journal (2019)
Lactobacillus crispatus represses vaginolysin expression by BV associated Gardnerella vaginalis and reduces cell cytotoxicity
Authors Joana Castro, Ana Paula Martins, Maria Elisa Rodrigues, Nuno Cerca Products EXTRACTME RNA BACTERIA & YEAST KIT (EM25.1) – To be discontinued, EXTRACTME RNA BACTERIA & YEAST KIT (EM25) – Discontinued Year 2018 Source 1075-9964/© 2018 Elsevier Ltd