The EXTRACTME GENOMIC DNA MICRO SPIN KIT is designed for a rapid and efficient purification of high quality genomic, mitochondrial, bacterial, parasite or viral DNA from solid tissues (fresh, frozen, formalin-preserved or paraffin-embedded), physiological fluids (urine, cerebrospinal fluid, peritoneal fluid, pleural fluid, sputum), fresh and frozen blood (human and mammalian), human and animal mucosa membrane swabs (including buccal, nasal, pharyngeal and vaginal swabs), semen, hair, insects, bacteria, yeast and cell cultures with an extremely low elution volume of only 5 μl. The isolation protocol and buffer formulations have beenoptimized for high isolation efficiency and DNA purity. The product is intended for research use only.

PRINCIPLE

The EXTRACTME GENOMIC DNA MICRO SPIN KIT utilizes Purification Micro Spin Columns with membranes which efficiently and selectively bind nucleic acids. In the first step, biological material is lysed by Proteinase K in optimized GL Buffer. At this stage all the cellular membranes and proteins are degraded. When it is necessary to remove RNA the use of RNase A is recommended. After the  addition of chaotropic salts, the lysate is applied to Purification Micro Spin Column’s membrane and the DNA is bound. The two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted with the use of  either a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and may be used directly in all downstream applications such as PCR, quantitive real-time PCR, pharmacogenic research, Southern blotting, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, DNA sequencing, enzymatic  restriction, ligation and so forth, or stored until ready to use.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL

• fresh or frozen solid tissue: up to 15 mg
• formalin-preserved tissue: up to 15 mg
• paraffin-embedded tissue: up to 15 mg
• phisiological fluids (urine, PMR, peritoneal fluid); up to 5 ml
• cell culture: up to 10⁷ cells
• broth or plate bacterial or yeast culture, single bacterial
• yeast colony, frozen cell pellet
• buccal, nasal, pharyngeal, vaginal, buccal saliva swabs or semen
• fresh or frozen blood: up to 0.15 ml
• hair: up to 15 mg
• insects: up to 15 mg

BINDING CAPACITY

6 μg DNA

TIME REQUIRED

• approx. 12 minutes (lysis time not included)
• 10-60 minutes for sample preparation

DNA PURITY

A₂₆₀/A₂₈₀ ratio = 1.7 – 1.9

RECOMMENDATIONS AND IMPORTANT NOTES