EXTRACTME DNA YEAST KIT (EM10)

EXTRACTME DNA YEAST KIT (EM10)

30,00 503,00 

The EXTRACTME DNA YEAST KIT is designed for a rapid and efficient purification of high quality DNA from broth and plate yeast cultures as well as frozen cells. The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity. The product is intended for research use only.
EM10, EM10-010, EM10-050, EM10-250

Available Options:

Exctractions

10 extractions, 250 extractions, 50 extractions

Product information

PRINCIPLE

DNA purification procedure consists of five steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. During the first step cell walls of the yeast cells are disintegrated in the YS Buffer (Spheroplast Buffer). Spheroplasts obtained this way are enzymatically lysed by Proteinase K in lysis buffer. To obtain an RNA-free DNA sample, RNA is removed by RNase A. After the addition of chaotropic salts, the lysate is applied to the purification column membrane and DNA is  bound. The two-step washing stage effectively  removes impurities and enzyme inhibitors. Purified DNA is eluted with then use of a low ionic strength buffer (Elution Buffer) or water (pH  7.0–9.0) and may be used directly in all downstream applications such as PCR, qPCR,  Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until  ready to use.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL

Broth or plate yeast culture, frozen cell pellet

EFFICIENCY

Up to 1 x 108 cells → 100%
2 x 108 ÷ 9 x 108 → 30–70%

BINDING CAPACITY

Approx. 40 μg DNA

TIME REQUIRED

Approx. 75 minutes (including incubation time)

DNA PURITY

A260/A280 ratio = 1.7 – 1.9

RECOMMENDATIONS AND IMPORTANT NOTES

Sample material
DNA isolation efficiency and purity can depend not only on the number of yeast cells, cell type (morphology) or antibiotics in the grow medium, but also on the age and condition of the yeast cells.

The kit is designed to isolate DNA from max. 1 x 108 yeast cells. Using greater number of cells may significantly reduce the isolation efficiency and the purity of the DNA.

Extracting DNA from fresh starting material or frozen cell pellets is recommended. To minimize DNA degradation, avoid subjecting the sample material to repeated freeze/thaw cycles. Using old or repeatedly frozen/thawed material may result in low efficiency isolation of high molecular DNA.

Protocol & Manual

EXTRACTME DNA YEAST KIT (EM10) - Manual

Technical Tips

EXTRACTME DNA YEAST KIT – TROUBLESHOOTING

FAQ

Quality & Safety

QUALITY CONTROL

The quality of each production batch (LOT) of the EXTRACTME DNA YEAST KIT is tested with the use of standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.

EXTRACTME DNA YEAST KIT (EM10) - MSDS


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