Available Options:
SKU | Exctractions | ||
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![]() | EM03-250 | 250 extractions | Ask for an offer |
![]() | EM03-050 | 50 extractions | Ask for an offer |
PRINCIPLE
DNA purification procedure consists of four steps and utilizes spin DNA Purification Columns with membranes which efficiently and selectively bind nucleic acids. In the first isolation step, tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then homogenate is lysed by Proteinase K in optimized TL Buffer. At this stage, all the cellular membranes and proteins are degraded. When a metabolically active tissue is used for isolation, RNA is removed by RNase A. Homogenate is separated from undigested tissue remains by centrifugation and combined with chaotropic salts. Mixture is then applied to DNA Purification Column membrane and DNA is bound. A two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted using a low ionic strength buffer or water (pH of 7.0–9.0) and can be used directly in all downstream applications such as qPCR, Southern blotting, DNA sequencing, enzymatic restriction, DNA ligation and so forth, or stored until ready to use.
PRODUCT SPECIFICATIONS
SAMPLE MATERIAL
- fresh or frozen solid tissue: 1–30 mg
- formalin-preserved tissue: 1–30 mg
- paraffin-embedded tissue: 1–30 mg
- cell culture: 103–107 cells
- physiological fluids (urine, PMR, peritoneal fluid): 1–5 ml
- hair: 10–30 mg
- insects: 1–30 mg
- rodent tail: up to 30 mg
BINDING CAPACITY
Approx. 50 μg DNA
TIME REQUIRED
- approx. 12 minutes (lysis time not included)
- 30–40 minutes for mechanical homogenization
- 1–16 hours for periodical shaking by vortexing
DNA PURITY
A260/A280 ratio = 1.7 – 1.9
RECOMMENDATIONS AND IMPORTANT NOTES
RNA contamination
Most fresh or frozen tissue contains more RNA than DNA, especially metabolically active tissues like glands, nerve tissue and epithelium. RNA may interfere with some enzymatic reactions, but does not inhibit PCR. If an RNA-free DNA sample is desired, add 4 μl of RNase A solution and incubate at 37°C for 5 minutes (step 3 of the Isolation Protocol, section XI).
EXTRACTME DNA TISSUE KIT (EM03) – Discontinued - Manual
EXTRACTME DNA TISSUE KIT – TROUBLESHOOTING
QUALITY CONTROL
The quality of each production batch (LOT) of the EXTRACTME DNA TISSUE KIT is tested using of standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.
EXTRACTME DNA TISSUE KIT (EM03) – Discontinued - MSDS
EXTRACTME DNA TISSUE KIT (EM03) – Discontinued - CoA 2.2017 L.626772
EXTRACTME DNA TISSUE KIT (EM03) – Discontinued - CoA 2.2017 L.815672
EXTRACTME DNA TISSUE KIT (EM03) – Discontinued - CoA 2.2017 L.746771
EXTRACTME DNA TISSUE KIT (EM03) – Discontinued - CoA 2.2017 L.866372
EXTRACTME DNA TISSUE KIT (EM03) – Discontinued - CoA 1.2018 L.765773
EXTRACTME DNA TISSUE KIT (EM03) – Discontinued - CoA 1.2018 L.765773
EXTRACTME DNA TISSUE KIT (EM03) – Discontinued - CoA 2.2017 L.616772
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Presence of Helicobacter pylori and H. suis DNA in Free-Range Wild Boars
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Distinct Expression Patterns of Two Tumor Necrosis Factor Superfamily Member 15 Gene Isoforms in Human Colon Cancer
Authors Tomasz Jerzy Ślebioda, Marcin Stanisławowski,Marta Cyman,Piotr Mieczysław Wierzbicki, Dorota Żurawa-Janicka, Jarek Kobiela, Wojciech Makarewicz, Marek Guzek, Zbigniew Kmieć Products EXTRACTME DNA TISSUE KIT (EM03) – Discontinued Year 2019 Source Digestive Diseases and Sciences, 2019