Available Options:
SKU | Exctractions | ||
---|---|---|---|
![]() | EM06-250 | 250 extractions | Ask for an offer |
![]() | EM06-050 | 50 extractions | Ask for an offer |
PRINCIPLE
DNA purification procedure consists of four steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. Swab or semen sample is subjected to enzymatic lysis by Proteinase K in SSL Buffer. When isolating from semen, DTT must also be used. In this step, cell walls, membranes and proteins are degraded by lysis buffer and Proteinase K. After addition of chaotropic salts, lysate is applied to purification minicolumn membrane and DNA is bound. A two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted with then use of a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, DNA ligation and so forth, or stored until ready to use.
PRODUCT SPECIFICATIONS
SAMPLE MATERIAL
Buccal, nasal, pharyngeal, vaginal, blood and saliva swabs or semen.
BINDING CAPACITY
Approx. 25 μg DNA
TIME REQUIRED
45-50 minutes (including incubation time)
DNA PURITY
A260/A280 ratio = 1.7 – 1.9
RECOMMENDATIONS AND IMPORTANT NOTES
Co-extracted vestigial RNA contamination may interfere with some enzymatic reaction, but does not inhibit PCR. If an RNA-free DNA sample is desired, add 4 μl of the RNase A solution (10 mg/ml; cat no. RP14, RP45) to SSL Buffer in step 2 of the Isolation Protocol. Mix well and incubate at 37°C for 5 minutes. After incubation, add Proteinase K and DTT when isolating from semen and continue isolation following the Isolation Protocol (section XI).
EXTRACTME DNA SWAB &SEMEN KIT (EM06) – Discontinued - Manual
EXTRACTME DNA SWAB & SEMEN KIT – TROUBLESHOOTING
QUALITY CONTROL
The quality of each production batch (LOT) of the EXTRACTME DNA SWAB & SEMEN KIT is tested using standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.
EXTRACTME DNA SWAB &SEMEN KIT (EM06) – Discontinued - MSDS
-
Identification of Genetic Variants in 65 Obesity Related Genes in a Cohort of Portuguese Obese Individuals
Authors Catarina Ginete, Bernardo Serrasqueiro, José Silva-Nunes, Luísa Veiga, Miguel Brito Products EXTRACTME DNA SWAB &SEMEN KIT (EM06) – Discontinued Year 2021 Source Genes 2021, 12(4), 603 -
Prediction of Early Childhood Caries Based on Single Nucleotide Polymorphisms Using Neural Networks
Authors Katarzyna Zaorska, Tomasz Szczapa, Maria Borysewicz-Lewicka, Michał Nowicki, Karolina Gerreth Products EXTRACTME DNA SWAB &SEMEN KIT (EM06) – Discontinued Year 2021 Source Genes 2021, 12, 462 -
Prediction of skin color, tanning and freckling from DNA in Polish population: linear regression, random forest and neural network approaches
Authors Katarzyna Zaorska, Piotr Zawierucha, Michał Nowicki Products EXTRACTME DNA SWAB &SEMEN KIT (EM06) – Discontinued Year 2019 Source M. Hum Genet (2019) 138: 635 -
ALTERAÇÕES NA MICROBIOTA VAGINAL DE VACAS HOLANDESAS CAUSADAS PELO USO DE IMPLANTE INTRAVAGINAL COM PROGESTERONA PARA SINCRONIZAÇÃO DO ESTRO
Authors Daniel Quadros, Arthur Nery da Silva, Giovana Ciacci Zanella, Fernanda L. Facioli, Lucas M. Lof, Carlos Bondan, Eraldo Lourenso Zanella, Ricardo Zanella Products EXTRACTME DNA SWAB &SEMEN KIT (EM06) – Discontinued Year 2017 Source 3º Congresso Regional de Medicina Veterinária - Anais Eletrônicos -
Association of ENAM gene single nucleotide polymorphisms with dental caries in Polish children
Authors Karolina Gerreth, Katarzyna Zaorska, Maciej Zabel, Maria Borysewicz-Lewicka, Michal Nowicki Products EXTRACTME DNA SWAB &SEMEN KIT (EM06) – Discontinued Year 2016 Source Clin Oral Invest (2016) 20:631–636