30,00 € – 440,00 €The EXTRACTME DNA SWAB & SEMEN KIT is designed for a rapid and efficient purification of high quality DNA from human and animal mucosa membrane swabs (including buccal, nasal, pharyngeal and vaginal swabs) as well as from semen. The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity. The product is intended for research use only.
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- Protocol & Manual
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10 extractions, 250 extractions, 50 extractions
DNA purification procedure consists of four steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. Swab or semen sample is subjected to enzymatic lysis by Proteinase K in SSL Buffer. When isolating from semen, DTT must also be used. In this step, cell walls, membranes and proteins are degraded by lysis buffer and Proteinase K. After addition of chaotropic salts, lysate is applied to purification minicolumn membrane and DNA is bound. A two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted with then use of a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, DNA ligation and so forth, or stored until ready to use.
Buccal, nasal, pharyngeal, vaginal, blood and saliva swabs or semen.
Approx. 25 μg DNA
45-50 minutes (including incubation time)
A260/A280 ratio = 1.7 – 1.9
RECOMMENDATIONS AND IMPORTANT NOTES
Co-extracted vestigial RNA contamination may interfere with some enzymatic reaction, but does not inhibit PCR. If an RNA-free DNA sample is desired, add 4 μl of the RNase A solution (10 mg/ml; cat no. RP14, RP45) to SSL Buffer in step 2 of the Isolation Protocol. Mix well and incubate at 37°C for 5 minutes. After incubation, add Proteinase K and DTT when isolating from semen and continue isolation following the Isolation Protocol (section XI).
Protocol & Manual
EXTRACTME DNA SWAB &SEMEN KIT (EM06) - Manual
EXTRACTME DNA SWAB & SEMEN KIT – TROUBLESHOOTING
Quality & Safety
The quality of each production batch (LOT) of the EXTRACTME DNA SWAB & SEMEN KIT is tested using standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.
EXTRACTME DNA SWAB &SEMEN KIT (EM06) - MSDS
ALTERAÇÕES NA MICROBIOTA VAGINAL DE VACAS HOLANDESAS CAUSADAS PELO USO DE IMPLANTE INTRAVAGINAL COM PROGESTERONA PARA SINCRONIZAÇÃO DO ESTRO
Authors: Daniel Quadros, Arthur Nery da Silva, Giovana Ciacci Zanella, Fernanda L. Facioli, Lucas M. Lof, Carlos Bondan, Eraldo Lourenso Zanella, Ricardo Zanella Products: EXTRACTME DNA SWAB & SEMEN KIT (EM06) Year: 2017 Source: 3º Congresso Regional de Medicina Veterinária - Anais Eletrônicos