EXTRACTME DNA GEL-OUT KIT (EM08)

EXTRACTME DNA GEL-OUT KIT (EM08)

21,00 208,00 

The EXTRACTME DNA GEL-OUT KIT is designed for a rapid and efficient purification of DNA fragments directly from agarose gels (standard and low-melting point agarose gels run in either a TAE or TBE buffer). Agarose, ethidium bromide and other contaminants from a sample are effectively removed in the purification process. The kit enables the purification of DNA fragments from 50 bp to 30 kb, as well as plasmid and genomic DNA. However, purification of fragments smaller than 100 bp and larger than 10 kb will result in decreased recovery rates. The purified DNA can be used in common downstream applications. The purification protocol and buffer formulations were optimized for high yields and DNA purity. The product is intended for research use only.

EM08, EM08-10, EM08-050, EM08-250, EM08-010

Available Options:

Additional information

Exctractions

10 extractions, 250 extractions, 50 extractions

Product information

PRINCIPLE

DNA purification procedure utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. In the first step, DNA fragment is excised from an agarose gel and incubated in GB Buffer, which enables gel fragment solubilization and protein degradation. As an added convenience, binding buffer contains a color indicator, which facilitates easy monitoring of the solution’s pH for optimal DNA binding. The two-step washing stage efficiently removes impurities and enzyme inhibitors. Purified DNA is eluted with the use either a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth or stored until ready to use.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL

Agarose fragment of up to 300 mg containing DNA

BINDING CAPACITY

Approx. 25 μg DNA

TIME REQUIRED

16-20 minutes

DNA PURITY

A260/A280 ratio = 1.7 – 1.9

RECOMMENDATIONS AND IMPORTANT NOTES

GB Buffer contains an indicator, which enables pH monitoring. Yellow indicates that the solution’s pH is lower than 7.5, which guarantees optimal DNA binding with the membrane. When the pH is higher than 7.5, the solution turns pink. It usually happens, for example, when the running buffer for electrophoresis has been used several times or was incorrectly prepared. In such cases, it is essential to add 10 μl of 3 M sodium acetate (pH 5.2). It will lower the pH, enabling the solution to bind efficiently to the minicolumn membrane.

 

Protocol & Manual

EXTRACTME DNA GEL-OUT KIT (EM08) - Manual

Technical Tips

EXTRACTME DNA GEL-OUT KIT – TROUBLESHOOTING

FAQ

Quality & Safety

QUALITY CONTROL

The quality of each production batch (LOT) of the EXTRACTME DNA GEL-OUT KIT is tested with the use of standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer

EXTRACTME DNA GEL-OUT KIT (EM08) - MSDS

EXTRACTME DNA GEL-OUT KIT (EM08) - MSDS

EXTRACTME DNA GEL-OUT KIT (EM08) - MSDS


EXTRACTME DNA GEL-OUT KIT (EM08) - CoA 1.2018 L.706671

EXTRACTME DNA GEL-OUT KIT (EM08) - CoA 3.2017 L.656572

EXTRACTME DNA GEL-OUT KIT (EM08) - CoA 1.2018 L.695873

Literature