24,00 € – 282,00 €The EXTRACTME DNA CLEAN-UP & GEL-OUT MICRO SPIN KIT is designed for a rapid and efficient purification of DNA fragments after enzymatic reactions and directly from agarose gels (standard and low-melting point agarose gels run in either a TAE or TBE buffer) with an extremely low elution volume of only 5 μl. It efficiently removes nucleases, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, agarose, ethidium bromide and other contaminants. The purified DNA can be used in common downstream applications. The kit enables the purification of DNA fragments from 35 bp to 30 kb for clean-up procedure and from 100 bp to 30 kbp for gel-out procedure, as well as plasmid and genomic DNA. However purification of fragments smaller than 50 bp and larger than 10 kb will result in decreased recovery rates. The purification protocol and buffer formulations were optimized for high yields and purity of DNA. The product is intended for research use only.
DNA purification procedure utilizes spin microcolumns with membranes which efficiently and selectively bind nucleic acids. In the first step of the clean-up protocol CB Buffer is added to a DNA sample. It causes proteins to degrade and enables DNA binding to the column membrane while in the gel-out protocol the DNA fragments is excised from an agarose gel and incubated in GB Buffer, which enables gel fragment solubilization and protein degradation. As an added convenience, the binding buffers contain a colour indicator, which facilitates easy monitoring of the solution’s pH for optimal DNA binding. The two-step washing stage efficiently removes impurities and enzyme inhibitors. Purified DNA is eluted with the use either a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth or stored until ready to use.
CLEAN-UP: DNA sample
GEL-OUT: agarose fragment of up to 150 mg containing DNA
Depending on DNA fragment length (in the range of 100 bp – 10 kb): 70–90 %
DNA FRAGMENT LENGTH
100 bp – 10 kb
DNA fragments in the 50–100 bp and 10–30 kbp range can also be purified, as can genomic and plasmid DNA, however the efficiency will be decreased.
Approx. 6 μg DNA
approx. 10 min for clean-up procedure
approx. 15 min for gel-out procedure
A260/A280 ratio = 1.7 – 1.9
RECOMMENDATIONS AND IMPORTANT NOTES
Protocol & Manual
EXTRACTME DNA CLEAN-UP & GEL-OUT MICRO SPIN KIT (EM28) - Manual
EXTRACTME DNA GEL-OUT KIT – TROUBLESHOOTING
Quality & Safety
The quality of each production batch (LOT) of the EXTRACTME DNA CLEAN-UP & GEL-OUT KIT is tested using standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.
EXTRACTME DNA CLEAN-UP & GEL-OUT MICRO SPIN KIT (EM28) - MSDS
Functional analysis of novel RUNX2 mutations identified in patients with cleidocranial dysplasia
Authors: Ewa Hordyjewska‐Kowalczyk, Anna Sowińska‐Seidler, Ewelina M. Olech, Magdalena Socha, Renata Glazar, Anna Kruczek, Anna Latos‐Bieleńska, Przemko Tylzanowski, Aleksander Jamsheer Products: EXTRACTME DNA CLEAN-UP & GEL-OUT MICRO SPIN KIT (EM28), dNTPs Year: 2019 Source: Clin Genet.; 1– 10