|EM28-250||250 extractions||Ask for an offer|
|EM28-050||50 extractions||Ask for an offer|
DNA purification procedure utilizes spin microcolumns with membranes which efficiently and selectively bind nucleic acids. In the first step of the clean-up protocol CB Buffer is added to a DNA sample. It causes proteins to degrade and enables DNA binding to the column membrane while in the gel-out protocol the DNA fragments is excised from an agarose gel and incubated in GB Buffer, which enables gel fragment solubilization and protein degradation. As an added convenience, the binding buffers contain a colour indicator, which facilitates easy monitoring of the solution’s pH for optimal DNA binding. The two-step washing stage efficiently removes impurities and enzyme inhibitors. Purified DNA is eluted with the use either a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth or stored until ready to use.
CLEAN-UP: DNA sample
GEL-OUT: agarose fragment of up to 150 mg containing DNA
Depending on DNA fragment length (in the range of 100 bp – 10 kb): 70–90 %
DNA FRAGMENT LENGTH
100 bp – 10 kb
DNA fragments in the 50–100 bp and 10–30 kbp range can also be purified, as can genomic and plasmid DNA, however the efficiency will be decreased.
Approx. 6 μg DNA
approx. 10 min for clean-up procedure
approx. 15 min for gel-out procedure
A260/A280 ratio = 1.7 – 1.9
EXTRACTME DNA CLEAN-UP & GEL-OUT MICRO SPIN KIT (EM28) - To be discontinued - Manual
EXTRACTME DNA GEL-OUT KIT – TROUBLESHOOTING
The quality of each production batch (LOT) of the EXTRACTME DNA CLEAN-UP & GEL-OUT KIT is tested using standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.
EXTRACTME DNA CLEAN-UP & GEL-OUT MICRO SPIN KIT (EM28) - To be discontinued - MSDS
Functional analysis of novel RUNX2 mutations identified in patients with cleidocranial dysplasia
Authors Ewa Hordyjewska‐Kowalczyk, Anna Sowińska‐Seidler, Ewelina M. Olech, Magdalena Socha, Renata Glazar, Anna Kruczek, Anna Latos‐Bieleńska, Przemko Tylzanowski, Aleksander Jamsheer Products EXTRACTME DNA CLEAN-UP & GEL-OUT MICRO SPIN KIT (EM28) – To be discontinued, Deoxyribonucleotides (dNTPs) Year 2019 Source Clin Genet.; 1– 10