EXTRACTME DNA BACTERIA KIT (EM02)

EXTRACTME DNA BACTERIA KIT (EM02)

30,00 446,00 

The EXTRACTME DNA BACTERIA KIT is designed for a rapid and efficient purification of high quality bacterial gDNA from broth and plate cultures as well as frozen cells. The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity. The product is intended for research use only.
EM02, EM02-010, EM02-050, EM02-250

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Product information

PRINCIPLE

DNA purification procedure consists of five steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. During the first step, cell walls, membranes and proteins are degraded by lysis buffer and Proteinase K. To obtain an RNA-free DNA sample, RNA is removed by RNase A. After addition of chaotropic salts, lysate is applied to purification column membrane and DNA is bound. A two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted with the use of a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and may be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, DNA ligation and so forth, or stored until ready to use.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL

Broth or plate bacterial culture, frozen cell pellet.

EFFICIENCY

  • up to 5 x 108 cells → 100%
  • 109 ÷ 3 x 109 → 75-90% (when the modified protocol for isolation from a great number of cells is followed)
  • 109 ÷ 3x 109 → ≤ 60% (when the standard isolation protocol is followed)

BINDING CAPACITY

Approx. 40 μg DNA

TIME REQUIRED

Approx. 40–60 minutes (including incubation time)

DNA PURITY

A260/A280 ratio = 1.7 – 1.9

Protocol & Manual

EXTRACTME DNA BACTERIA KIT (EM02) - Manual

Technical Tips

EXTRACTME DNA BACTERIA KIT – TROUBLESHOOTING

RECOMMENDATIONS AND IMPORTANT NOTES

Sample material DNA isolation efficiency and purity can depend not only on number of bacterial cells, cell type (morphology) or antibiotics in grow medium, but also on age andcondition  of the cells.
The kit is designed to isolate DNA from max. 3 x 109 bacterial cells. Using a greaternumber  of cells may significantly reduce isolation efficiency and purity of DNA. Extracting DNA from fresh starting material or frozen cell pellets is recommended. To minimize DNA degradation, avoid repeated freeze/thaw cycles of samples. Using old or repeatedly frozen/thawed material may result in low efficiency isolation of high molecular DNA.

Quality & Safety

QUALITY CONTROL

The quality of each production batch (LOT) of EXTRACTME DNA BACTERIA KIT is tested using standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.

EXTRACTME DNA BACTERIA KIT (EM02) - MSDS


EXTRACTME DNA BACTERIA KIT (EM02) - CoA 1.2017 L.626371

EXTRACTME DNA BACTERIA KIT (EM02) - CoA 1.2017 L.756172

EXTRACTME DNA BACTERIA KIT (EM02) - CoA 1.2017 L.736472

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