30,00 € – 446,00 €The EXTRACTME DNA BACTERIA KIT is designed for a rapid and efficient purification of high quality bacterial gDNA from broth and plate cultures as well as frozen cells. The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity. The product is intended for research use only.
DNA purification procedure consists of five steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. During the first step, cell walls, membranes and proteins are degraded by lysis buffer and Proteinase K. To obtain an RNA-free DNA sample, RNA is removed by RNase A. After addition of chaotropic salts, lysate is applied to purification column membrane and DNA is bound. A two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted with the use of a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and may be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, DNA ligation and so forth, or stored until ready to use.
Broth or plate bacterial culture, frozen cell pellet.
- up to 5 x 108 cells → 100%
- 109 ÷ 3 x 109 → 75-90% (when the modified protocol for isolation from a great number of cells is followed)
- 109 ÷ 3x 109 → ≤ 60% (when the standard isolation protocol is followed)
Approx. 40 μg DNA
Approx. 40–60 minutes (including incubation time)
A260/A280 ratio = 1.7 – 1.9
Protocol & Manual
EXTRACTME DNA BACTERIA KIT (EM02) - Manual
EXTRACTME DNA BACTERIA KIT – TROUBLESHOOTING
RECOMMENDATIONS AND IMPORTANT NOTES
Sample material DNA isolation efficiency and purity can depend not only on number of bacterial cells, cell type (morphology) or antibiotics in grow medium, but also on age andcondition of the cells.
The kit is designed to isolate DNA from max. 3 x 109 bacterial cells. Using a greaternumber of cells may significantly reduce isolation efficiency and purity of DNA. Extracting DNA from fresh starting material or frozen cell pellets is recommended. To minimize DNA degradation, avoid repeated freeze/thaw cycles of samples. Using old or repeatedly frozen/thawed material may result in low efficiency isolation of high molecular DNA.
Quality & Safety
The quality of each production batch (LOT) of EXTRACTME DNA BACTERIA KIT is tested using standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.
EXTRACTME DNA BACTERIA KIT (EM02) - MSDS
EXTRACTME DNA BACTERIA KIT (EM02) - CoA 1.2017 L.626371
EXTRACTME DNA BACTERIA KIT (EM02) - CoA 1.2017 L.756172
EXTRACTME DNA BACTERIA KIT (EM02) - CoA 1.2017 L.736472
Intra-operative biopsy in chronic sinusitis detects pathogenic Escherichia coli that carry fimG/H, fyuA and agn43 genes coding biofilm formation
Authors: Michał Michalik, Alfred Samet, Andrzej Marszałek, Beata Krawczyk, Roman Kotłowski, Alex Nowicki, Tomasz Anyszek, Stella Nowicki, Józef Kur, Bogdan Nowicki Products: EXTRACTME DNA BACTERIA KIT (EM02) Year: 2018 Source: PLOS ONE
New nuclease from extremely psychrophilic microorganism Psychromonas ingrahamii 37: identification and characterization
Authors: Maciejewska, N., Walkusz, R., Olszewski, M., & Szymańska, A Products: EXTRACTME DNA BACTERIA KIT (EM02), Pwo DNA polymerase, EXTRACTME DNA GEL-OUT KIT (EM08) – DISCONTINUED, EXTRACTME DNA CLEAN-UP KIT (EM07) – DISCONTINUED, ExtractMe Plasmid DNA Kit Year: 2018 Source: Molecular biotechnology, 61(2), 122-133.
Lactococcus lactis as a safe and inexpensive source of bioactive silver composites
Authors: Railean-Plugaru Viorica, Pomastowski Pawel, Meller Kinga, Złoch Michal, Rafinska Katarzyna, Buszewski Boguslaw Products: EXTRACTME DNA BACTERIA KIT (EM02) Year: 2017 Source: Appl Microbiol Biotechnol (2017) 101:7141–7153
Monitoring of bacterial pathogens at workplaces in power plant using biochemical and molecular methods
Authors: Anna Ławniczek‑Wałczyk, Małgorzata Gołofit‑Szymczak, Marcin Cyprowski, Agata Stobnicka, Rafał L. Górny Products: EXTRACTME DNA BACTERIA KIT (EM02), TaqNova DNA Polymerase (RP702) Year: 2017 Source: Int Arch Occup Environ Health (2017) 90:285–295
Biofilm production and other virulence factors in Streptococcus spp. isolated from clinical cases of bovine mastitis in Poland
Authors: Edyta Kaczorek, Joanna Małaczewska, Roman Wójcik and Andrzej Krzysztof Siwicki Products: EXTRACTME DNA BACTERIA KIT (EM02) Year: 2017 Source: BMC Veterinary Research (2017) 13:398
Biosorption of silver cations onto Lactococcus lactis and Lactobacillus casei isolated from dairy products
Authors: Maciej Milanowski, Paweł Pomastowski, Viorica Railean-Plugaru, Katarzyna Rafińska, Tomasz Ligor, Bogusław Buszewski Products: EXTRACTME DNA BACTERIA KIT (EM02), Ideal Marker DNA M10Kpz Year: 2016 Source: Plos ONE
Fine-tuning recA expression in Staphylococcus aureus for antimicrobial photoinactivation: importance of photo-induced DNA damage in the photoinactivation mechanism
Authors: Mariusz Grinholc, Aleksandra Rodziewicz, Katarzyna Forys, Aleksandra Rapacka-Zdonczyk, Anna Kawiak, Anna Domachowska, Grzegorz Golunski, Christiane Wolz, Lili Mesak, Karsten Becker, Krzysztof P. Bielawski Products: EXTRACTME DNA BACTERIA KIT (EM02) Year: 2015 Source: Appl Microbiol Biotechnol (2015) 99:9161–9176
Identification and characterization of single-stranded DNA-binding protein from the facultative psychrophilic bacteria Pseudoalteromonas haloplanktis
Authors: Marcin Olszewski, Marta Nowak, Anna Cyranka-Czaja, Józef Kur Products: EXTRACTME DNA BACTERIA KIT (EM02), EXTRACTME DNA GEL-OUT KIT (EM08), EXTRACTME DNA CLEAN-UP KIT (EM07), Pwo DNA polymerase, PCR Reagents Year: 2014 Source: Microbiological Research 169 (2014) 139– 147
Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum
Authors: Marta Nowak, Marcin Olszewski, Marta Śpibida and Józef Kur Products: EXTRACTME DNA BACTERIA KIT (EM02), EXTRACTME DNA GEL-OUT KIT (EM08), EXTRACTME DNA CLEAN-UP KIT (EM07), Pwo DNA polymerase, PCR Reagents Year: 2014 Source: Nowak et al. BMC Microbiology 2014, 14:91