EasyPCR I (DY45) – Discontinued

EasyPCR I (DY45) – Discontinued

EasyPCRI Kit -aim: presentation of an influence of changing reaction components onto the reaction efficiency. The following will be tested: composition of the reaction buffer, magnesium and nucleotides (dNTPs) concentration and the amount of template. The EasyPCRI kit is designed to perform experiments during two laboratory classes (I - PCR set-up - 2 hours; II - electrophoresis - 2 hours). We suggest to perform laboratory experiments in six groups of two students.
DY45, DY455

Available Options:

EasyPCR I kit components (1 exercise, DY45):

6 sets of PCR reagents:

  • 5 tubes with 10x reaction buffer (20 reactions per tube)
  • 5 tubes with MgCl2 solution (20 reactions per tube)
  • 2 tubes with RED polymerases (3 reactions per tube)
  • 2 tubes with Master Mix RED (3 reactions per tube)
  • 12 tubes with ready-to-use dNTPs mixture (10 reactions per tube)
  • 6 tubes with FORWARD primer (20 reactions per tube)
  • 6 tubes with REVERSE primer (20 reactions per tube)
  • 6 tubes with PCR – grade water
  • 6 tubes with thermostable DNA polymerase (20 reactions per tube)
  • 6 tubes with DNA template (10 reactions per tube)

Moreover:

  • 7 laboratory protocols (one with additional information for a teacher)
  • Agarose (5 g)
  • 50x TAE buffer for agarose gel electrophoresis (50 ml)
  • M100-500 ready-to-use DNA molecular weight standard for 10 electrophoretic lanes
  • 6x GREEN DNA Gel Loading Buffer (0.5 ml)

EasyPCR I kit components (5 exercises, DY455):

6 sets of PCR reagents:

  • 5 tubes with 10x reaction buffer (100 reactions per tube)
  • 5 tubes with MgCl2 solution (100 reactions per tube)
  • 2 tubes with RED polymerases (15 reactions per tube)
  • 2 tubes with Master Mix RED (15 reactions per tube)
  • 12 tubes with ready-to-use dNTPs mixture (50 reactions per tube)
  • 6 tubes with FORWARD primer (100 reactions per tube)
  • 6 tubes with REVERSE primer (100 reactions per tube)
  • 6 tubes with PCR – grade water
  • 6 tubes with thermostable DNA polymerase (100 reactions per tube)
  • 6 tubes with DNA template (50 reactions per tube)

Moreover:

  • 7 laboratory protocols (one with additional information for a teacher)
  • Agarose (25 g)
  • 50x TAE buffer for agarose gel electrophoresis (100 ml)
  • M100-500 ready-to-use DNA molecular weight standard for 50 electrophoretic lanes
  • 6x GREEN DNA Gel Loading Buffer (1 ml)

Additional equipment and materials required (not included in the kit):

  • Automatic pipettes with appropriate tips
  • PCR tubes and 1.5 ml Eppendorf tubes
  • UV transilluminator or lamp
  • Agarose electrophoresis equipment (apparatus, power supply)
  • Microwave oven or laboratory burner
  • Thermal cycler
  • Ethidium bromide solution or other stain for DNA visualization
  • Nitrile gloves

Shipping conditions

  • Reagents for DNA isolation are shipped at room temperature.
  • Reagents for PCR set up are shipped on dry or blue ice.
  • DNA templates are shipped on dry or blue ice.
  • DNA Ladder is shipped on dry or blue ice.
  • Other reagents for electrophoresis are shipped at room temperature.