The dNTPs is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates (dATP, dCTP, dGTP, dTTP), ready to use in PCR, RT-PCR and Real-Time PCR applications. The dNTPs are available as a set of four separate buffered dNTPs solutions (dNTPs SET) and also as an accurate mixture (dNTPs MIX). Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.

Deoxyribonucleotides are a crucial component of PCR reaction and their chemical purity has a significant influence on the PCR reaction results. They are supplied as lithium salts solutions, which provides extended stability and resistance to repeated freezing and thawing cycles.

Features and advantages

• The highest purity, determined by means of enzymatic synthesis and high resolution HPLC (> 99 % dNTP, < 0.9 % dNDP)
• Increased sensitivity and yield; allows the detection of a small number of copies of template DNA
• DNase, RNase, ATPase and pyrophosphatase free
• Effectively and efficiently amplifies even very large PCR amplicons
• The presence of lithium salt in the storage buffer guarantees high stability and resistance to frequent thawing and freezing cycles
• Free of modified dNTP, tetraphosphorane and pirophosphorane contaminations, which often cause an inhibition of the PCR reaction

Applications

• PCR, long-PCR, Real-time PCR, RT-PCR
• cDNA synthesis
• Primer extension
• DNA sequencing and labeling