dNTPs SET 10 mM (RP665)

dNTPs SET 10 mM (RP665)

82,00 

dNTPs SET 10 mM is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates 10 mM of each dNTP (separately: dATP, dCTP, dGTP, dTTP), supplied as lithium salts solutions. (much higher stability) Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.
RP665

Available Options:

The dNTPs is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates (dATP, dCTP, dGTP, dTTP), ready to use in PCR, RT-PCR and Real-Time PCR applications. The dNTPs are available as a set of four separate buffered dNTPs solutions (dNTPs SET) and also as an accurate mixture (dNTPs MIX). Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.

Deoxyribonucleotides are a crucial component of PCR reaction and their chemical purity has a significant influence on the PCR reaction results. They are supplied as lithium salts solutions, which provides extended stability and resistance to repeated freezing and thawing cycles.

Features and advantages

  • The highest purity, determined by means of enzymatic synthesis and high resolution HPLC (> 99 % dNTP, < 0.9 % dNDP)
  • Increased sensitivity and yield; allows the detection of a small number of copies of template DNA
  • DNase, RNase, ATPase and pyrophosphatase free
  • Effectively and efficiently amplifies even very large PCR amplicons
  • The presence of lithium salt in the storage buffer guarantees high stability and resistance to frequent thawing and freezing cycles
  • Free of modified dNTP, tetraphosphorane and pirophosphorane contaminations, which often cause an inhibition of the PCR reaction

Applications

  • PCR, long-PCR, Real-time PCR, RT-PCR
  • cDNA synthesis
  • Primer extension
  • DNA sequencing and labeling

dNTPs SET 10 mM (RP665) - Manual

Storage conditions

Store at -20°C upon receipt. Avoid multiple freezing/thawing cycles.
Aliquoting is recommended.

Shipping conditions

Shipping on dry or blue ice.

dNTPs SET 10 mM (RP665) - MSDS