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The dNTPs is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates (dATP, dCTP, dGTP, dTTP), ready to use in PCR, RT-PCR and Real-Time PCR applications. The dNTPs are available as a set of four separate buffered dNTPs solutions (dNTPs SET) and also as an accurate mixture (dNTPs MIX). Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.
Deoxyribonucleotides are a crucial component of PCR reaction and their chemical purity has a significant influence on the PCR reaction results. They are supplied as lithium salts solutions, which provides extended stability and resistance to repeated freezing and thawing cycles.
Features and advantages
- The highest purity, determined by means of enzymatic synthesis and high resolution HPLC (> 99 % dNTP, < 0.9 % dNDP)
- Increased sensitivity and yield; allows the detection of a small number of copies of template DNA
- DNase, RNase, ATPase and pyrophosphatase free
- Effectively and efficiently amplifies even very large PCR amplicons
- The presence of lithium salt in the storage buffer guarantees high stability and resistance to frequent thawing and freezing cycles
- Free of modified dNTP, tetraphosphorane and pirophosphorane contaminations, which often cause an inhibition of the PCR reaction
- PCR, long-PCR, Real-time PCR, RT-PCR
- cDNA synthesis
- Primer extension
- DNA sequencing and labeling
dNTPs MIX 10 mM (RP63) - Manual
Store at -20°C upon receipt. Avoid multiple freezing/thawing cycles.
Aliquoting is recommended.
Shipping on dry or blue ice.
Digital Rolling Circle Amplification–Based Detection of Ebola and Other Tropical Viruses
Authors Sibel Ciftci, Felix Neumann, Samir Abdurahman, K. Sofia Appelberg, Ali Mirazimi, Mats Nilsson, Narayanan Madaboosi Products RNase H (RT34), Deoxyribonucleotides (dNTPs) Year 2020 Source The Journal of Molecular Diagnostics, Volume 22, Issue 2
Complete sequence analysis of human toll-like receptor 3 gene in natural killer cells of multiple sclerosis patients
Authors Elie Deeba, Dana Koptides, Anastasia Lambrianides, Marios Pantzaris, George Krashias, Christina Christodoulou Products Deoxyribonucleotides (dNTPs) Year 2019 Source Multiple Sclerosis and Related Disorders, Volume 33, 2019, Pages 100-106
A novel mutation tolerant padlock probe design for multiplexed detection of hypervariable RNA viruses
Authors Sibel Ciftci, Felix Neumann, Iván Hernández-Neuta, Mikhayil Hakhverdyan, Ádám Bálint, David Herthnek, Narayanan Madaboosi & Mats Nilsson Products RNase H (RT34), T4 DNA Ligase (EN11), Deoxyribonucleotides (dNTPs), ATP Year 2019 Source Scientific Reports, 2019, Vol 9, Article number: 2872
Antibiotic Susceptibility and Virulence Factors in Escherichia coli from Sympatric Wildlife of the Apuan Alps Regional Park (Tuscany, Italy)
Authors Barbara Turchi, Marta Dec, Fabrizio Bertelloni, Stanisław Winiarczyk, Sebastian Gnat, Flavio Bresciani, Fabio Viviani, Domenico Cerri, Filippo Fratini Products Deoxyribonucleotides (dNTPs) Year 2019 Source Microbial Drug Resistance; 25:5, 772-780
Functional analysis of novel RUNX2 mutations identified in patients with cleidocranial dysplasia
Authors Ewa Hordyjewska‐Kowalczyk, Anna Sowińska‐Seidler, Ewelina M. Olech, Magdalena Socha, Renata Glazar, Anna Kruczek, Anna Latos‐Bieleńska, Przemko Tylzanowski, Aleksander Jamsheer Products EXTRACTME DNA CLEAN-UP & GEL-OUT MICRO SPIN KIT (EM28) – To be discontinued, Deoxyribonucleotides (dNTPs) Year 2019 Source Clin Genet.; 1– 10
Efficient DNA-assisted synthesis of trans-membrane gold nanowires
Authors Maoxiang Guo, Iván Hernández-Neuta, Narayanan Madaboosi, Mats Nilsson and Wouter van der Wijngaart Products T4 DNA Ligase (EN11), Deoxyribonucleotides (dNTPs), ATP Year 2018 Source Nucleic Acids Research, 2017, Vol. 45, No. 8 e59
Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy
Authors Malte Kühnemund Qingshan Wei, Evangelia Darai, Yingjie Wang, Iván Hernández-Neuta, Zhao Yang, Derek Tseng, Annika Ahlford, Lucy Mathot, Tobias Sjöblom, Aydogan Ozcan & Mats Nilsson Products RNase H (RT34), T4 DNA Ligase (EN11), TRANSCRIPTME RNA Kit (RT31), Deoxyribonucleotides (dNTPs) Year 2017 Source NATURE COMMUNICATIONS
Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production
Authors Ewa Skała, Renata Grąbkowska, Przemysław Sitarek, Łukasz Kuźma, Andrzej Błauż, Halina Wysokińska Products TaqNova DNA Polymerase (RP7A), Deoxyribonucleotides (dNTPs), Primers Year 2015 Source Plant Cell Tiss Organ Cult (2015) 123:83–98
Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives
Authors Ewa Skała, Agnieszka Kicel, Monika A. Olszewska, Anna K. Kiss, and Halina Wysokińska Products TaqNova DNA Polymerase (RP7A), Deoxyribonucleotides (dNTPs), Primers Year 2015 Source Hindawi Publishing Corporation BioMed Research International Volume 2015, Article ID 181098, 11 pages
Identification of Lactobacillus strains of goose origin using MALDI-TOF mass spectrometry and 16Se23S rDNA intergenic spacer PCR analysis
Authors Marta Dec, Renata Urban-Chmiel, Sebastian Gnat, Andrzej Puchalski, Andrzej Wernicki Products , Deoxyribonucleotides (dNTPs), , Primers Year 2014 Source Elsevier
The use of long-term Scutellaria altissima callus cultures for shoot regeneration, production of bioactive metabolites and micropropagation
Authors Izabela Grzegorczyk-Karolak, Łukasz Kuźma and Halina Wysokińska Products Deoxyribonucleotides (dNTPs), Taq Polymerases, Primers Year 2013 Source Journal of Medicinal Plant Research