The dNTPs is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates (dATP, dCTP, dGTP, dTTP), ready to use in PCR, RT-PCR and Real-Time PCR applications. The dNTPs are available as a set of four separate buffered dNTPs solutions (dNTPs SET) and also as an accurate mixture (dNTPs MIX). Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.
Deoxyribonucleotides are a crucial component of PCR reaction and their chemical purity has a significant influence on the PCR reaction results. They are supplied as lithium salts solutions, which provides extended stability and resistance to repeated freezing and thawing cycles.
Features and advantages
- The highest purity, determined by means of enzymatic synthesis and high resolution HPLC (> 99 % dNTP, < 0.9 % dNDP)
- Increased sensitivity and yield; allows the detection of a small number of copies of template DNA
- DNase, RNase, ATPase and pyrophosphatase free
- Effectively and efficiently amplifies even very large PCR amplicons
- The presence of lithium salt in the storage buffer guarantees high stability and resistance to frequent thawing and freezing cycles
- Free of modified dNTP, tetraphosphorane and pirophosphorane contaminations, which often cause an inhibition of the PCR reaction
- PCR, long-PCR, Real-time PCR, RT-PCR
- cDNA synthesis
- Primer extension
- DNA sequencing and labeling
dNTPs MIX 10 mM (RP63) - Manual
Store at -20°C upon receipt. Avoid multiple freezing/thawing cycles.
Aliquoting is recommended.
Shipping on dry or blue ice.
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Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy
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Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production
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Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives
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Identification of Lactobacillus strains of goose origin using MALDI-TOF mass spectrometry and 16Se23S rDNA intergenic spacer PCR analysis
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The use of long-term Scutellaria altissima callus cultures for shoot regeneration, production of bioactive metabolites and micropropagation
Authors Izabela Grzegorczyk-Karolak, Łukasz Kuźma and Halina Wysokińska Products Deoxyribonucleotides (dNTPs), Taq Polymerases, Primers Year 2013 Source Journal of Medicinal Plant Research