A model of proteins’ molecular weight for Blue Prestained Protein Marker is a mixture of carefully selected 10 stained, proteins within a wide range of masses (10 –180 kDa). Proteins, the marker consists of, are covalently bonded with the blue dye. Two reference fragments are (25 kDa and 72 kDa) more intense during SDS-PAGE electrophoresis (buffer Tris-Glycine). The Marker is ready to use and does not require any additional prepartions before it is transferred to the wells in a polyacrylamide gel.
- Proteins’ molecular weight estimation during polyacrylmide electrophoressis in denaturation condtions (SDS-PAGE) and Western blotting
- Verification of the efficiency of proteins transfer to PVDF, nylon, nitrocellulose membranes (Western blotting)
- Monitoring of proteins migration process during SDS-PAGE electrophoresis
- Covers a wide range of masses: 10–180 kDa
- Ready to use format
- Allows to monitor Markers’ proteins migration process during electrophoressis
- Marker creates ten bold bands in a ranges of 10–180 kDa
Blue Prestained Protein Marker (10–180 kDa) - Discontinued - Manual
0.1– 0.5 mg/ml of each protein suspended in 20 mM Tris-phosphate (pH 7.5); 2% SDS; 0.2 mM DTT; 3.6 M urea and 15% (v/v) glycerol.
- Prior to use, thaw the Protein Marker at room temperature or 37°C.
- Do not heat, dilute or add any reducing agent!
- In order to obtain best results, depending on the type of gel, size of a well and aplication it is recommended to transfer:
- SDS-PAGE: 3 μl to 5 μl
- Western blotting: 1.5 μl to 2.5 μl
- Marker’s low molecular weight proteins may migrate with the same
electrophoretic speed as a loading dye used in low percentage gels (< 10%).
- Marker’s high molecular weight proteins (> 100 kDa) may require
a higher voltage or longer transfer time during Western Blotting.
Store at 2–8°C (up to 3 months).Longer storage time requires temperature equal to -20°C.
Shipping on blue or dry ice.
Tested during SDS-PAGE electrophoresis and Western blotting.