Blue Prestained Protein Marker (10–180 kDa)

Blue Prestained Protein Marker (10–180 kDa)

122,00 

A model of proteins’ molecular weight for Blue Prestained Protein Marker is a mixture of carefully selected 10 stained, proteins within a wide range of masses (10 –180 kDa). Proteins, the marker consists of, are covalently bonded with the blue dye. Two reference fragments are (25 kDa and 72 kDa) more intense during SDS-PAGE electrophoresis (buffer Tris-Glycine). The Marker is ready to use and does not require any additional prepartions before it is transferred to the wells in a polyacrylamide gel.
PM32-500

SKU: PM32 Categories: ,

Available Options:

Volume

500 μl

Product Information

A model of proteins’ molecular weight for Blue Prestained Protein Marker is a mixture of carefully selected 10 stained, proteins within a wide range of masses (10 –180 kDa). Proteins, the marker consists of, are covalently bonded with the blue dye. Two reference fragments are (25 kDa and 72 kDa) more intense during SDS-PAGE electrophoresis (buffer Tris-Glycine). The Marker is ready to use and does not require any additional prepartions before it is transferred to the wells in a polyacrylamide gel.

Application

  • Proteins’ molecular weight estimation during polyacrylmide electrophoressis in denaturation condtions (SDS-PAGE) and Western blotting
  • Verification of the efficiency of proteins transfer to PVDF, nylon, nitrocellulose membranes (Western blotting)
  • Monitoring of proteins migration process during SDS-PAGE electrophoresis

Advantages

  •  Covers a wide range of masses: 10–180 kDa
  • Ready to use format
  • Allows to monitor Markers’ proteins migration process during electrophoressis
  • Marker creates ten bold bands in a ranges of 10–180 kDa

Protocol & Manual

Blue Prestained Protein Marker (10–180 kDa) - Manual

Technical Tips

Formulation

0.1– 0.5 mg/ml of each protein suspended in 20 mM Tris-phosphate (pH 7.5); 2% SDS; 0.2 mM DTT; 3.6 M urea and 15% (v/v) glycerol.

Usage

  • Prior to use, thaw the Protein Marker at room temperature or 37°C.
  • Do not heat, dilute or add any reducing agent!
  • In order to obtain best results, depending on the type of gel, size of a well and aplication it is recommended to transfer:
    • SDS-PAGE: 3 μl to 5 μl
    • Western blotting: 1.5 μl to 2.5 μl

Additional information

  • Marker’s low molecular weight proteins may migrate with the same
    electrophoretic speed as a loading dye used in low percentage gels (< 10%).
  • Marker’s high molecular weight proteins (> 100 kDa) may require
    a higher voltage or longer transfer time during Western Blotting.

Storage conditions

Store at 2–8°C (up to 3 months).Longer storage time requires temperature equal to -20°C.

Shipping conditions

Shipping on blue or dry ice.

FAQ

Quality & Safety

Quality control

Tested during SDS-PAGE electrophoresis and Western blotting.

Blue Prestained Protein Marker (10–180 kDa) - MSDS


Blue Prestained Protein Marker (10–180 kDa) - CoA 1.2018 L.815972

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