119,00 € – 777,00 €The AMPLIFYME SG Universal Mix is a convenient enzyme mixture for fast and reliable quantitative Real-Time PCR, using SG dsDNA-binding dye. Compatible with all types of qPCR instruments. Additional tubes with low and high concentration of ROX are included.
Ready-to-use, 2x concentrated Master Mix contains TaqNovaHS polymerase which is a mixture of recombinant Taq polymerase over-expressed in E. coli and a highly specific monoclonal anti-Taq antibody. The TaqNovaHS polymerase enables easy set-up of a hot-start PCR reaction at room temperature. The antibody binds reversibly to the enzyme, inhibiting polymerase activity at ambient temperatures, what prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR set-up. The antibody is released from the polymerase during initial DNA denaturation step, thus providing full DNA polymerase activity during standard cycling conditions.
Precisely optimized buffer components, reliable DNA polymerase and the appropriate selection of PCR enhancers provide a high performance, sensitivity and specificity over a broad dynamic range and give consistent results across all commonly-used Real-Time PCR platforms.
- qPCR / Real-Time PCR
- gene expression analysis
- mass screening
- pathogen detection
- characterization of genetically modified organisms (GMO)
- copy number variation analysis
Quality & Safety
The AMPLIFYME SG No-ROX Mix is extensively tested for its performance in different Real-Time PCR assays. Free of deoxyribonuclease contamination.
AMPLIFYME SG UNIVERSAL Mix (AM02) - MSDS
AMPLIFYME SG UNIVERSAL Mix (AM02) - CoA L:706373
AMPLIFYME SG UNIVERSAL Mix (AM02) - CoA L: 726373
AMPLIFYME SG UNIVERSAL Mix (AM02) - CoA L:716373
Clinical Value of Circulating Microribonucleic Acids miR-1 and miR-21 in Evaluating the Diagnosis of Acute Heart Failure in Asymptomatic Type 2 Diabetic Patients
Authors: Mutaa Abdalmutaleb Al-Hayali, Volkan Sozer, Sinem Durmus, Fusun Erdenen, Esma Altunoglu, Remise Gelisgen, Pınar Atukeren, Palmet Gun Atak, Hafize Uzun Products: AMPLIFYME SG UNIVERSAL Mix (AM02), EXTRACTME miRNA KIT (EM12) Year: 2019 Source: Biomolecules 2019, 9(5), 193