Name: Agarose HR (AG42)
SKU: AG42
Availability: InStock

176,00 € – 281,00 €

The HR Agarose is suitable for the separation of small DNA fragments and PCR products of between 20 – 800 bp. The HR Agarose allows the separation of DNA fragments differing by a molecular weight of 2% and provides a good alternative to polyacrylamide electrophoresis. In addition, HR Agarose has an excellent clarity of gels at a concentration of as high as 5%. This provides electrophoresis results of a very good clarity. The melting and gelling point of the agarose have lower values when compared to LE Agarose Standard and are higher than LM Agarose Low Melting Point.

AG42, AG42-005, AG-010

SKU: AG42 Categories: Agaroses, Electrophoresis

Available Options:

Features and advantages

Ultra-high resolution, with high clarity and low background
Medium melting and gelling point values
High gel strength(eas y-to-handle gels)
No DNase, RNase and protease activity
High purity (Molecular Biology Grade)

Applications

Conventional and preparative electrophoresis of DNA and RNA fragments
Ideal for PCR product separations
Purification of DNA fragments from the gel for further molecular biology applications
Analysis of AFLP (Amplified Fragment Length Polymorphism), STR (Short Tandem Repeats) and tri-/tetranucleotide repeats

Storage

Store at room temperature

Shipping conditions

Shipping at ambient temperature

Agarose HR (AG42) - Manual

Usage

Dissolve the appropriate quantity of agarose in 1x TAE / 1x TBE buffer by heating the suspension in a microwave or water bath. However, the best quality of the high percentage gels (4 – 5%) can be obtained by autoclaving the suspension for 10 min at 121°C. Cool the solution to approx. 60°C before pouring.
The detection of nucleic acids in agarose gels can be carried out with ethidium bromide or other commercially available stains for DNA visualization.