The 5x GC-Additive is an ideal tool for the amplification of GC-rich templates. It changes DNA behaviour upon heating and can be used with primer-template pairs with a GC-rich content which do not work well under standard PCR conditions.
The 5x GC-Additive reduces the number of secondary structures and enables the specific hybridisation of primers. In order to obtain the most satisfactory results, using it with TaqNova DNA Polymerase is recommended.
Features and advantages
- Ideal for GC-rich and difficult templates which fail with standard Taq DNA polymerases
- Improved PCR specificity and efficiency
5x GC-Additive (RP516) – Discontinued - Manual
5x GC-Additive should be used at 1.0 – 2.0x final concentration – the optimal amount required should be determined for each individual experiment.
Store all components at -20°C.
Shipping on dry or blue ice.
Free of unspecific nucleases.