2x PCR Hypernova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR Hypernova-RED solution contains a reaction buffer, magnesium chloride, dNTPs, Hypernova polymerase isolated from Pyrococcus woesei and enzymes increasing amplification efficiency. In order to set up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water.

This convenient 2x PCR Hypernova-RED master mix reduces the time required to set up PCR reactions and decreases the possibility of contamination, particularly when preparing large numbers of reactions. Moreover, the master mix is supplemented with an inner dye and a density reagent, which allows for direct loading of PCR products to a gel.

Features and advantages

• Increased processivity (for long amplicons, up to 10 kb)
• High fidelity (proofreading activity)
• Ideal for difficult templates which fail with standard Taq DNA polymerases
• Fool proof during multiplex PCR
• Very specific and sensitive
• More thermostable than Taq polymerase
• Facilitate high throughput PCR reaction set-up
• Reduced risk of contamination
• Direct gel loading

Applications

• Reproducible amplification of long templates
• Multiplex PCR
• Cloning, site-directed mutagenesis and other methods which require high fidelity
• Amplification of regions abundant in GC

Unit definition

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 72°C in a 50 μl reaction.