The RIBOPROTECT Hu-Mut is a genetically modified human placental RNase inhibitor expressed in Escherichia coli. The RIBOPROTECT Hu-Mut has significantly improved resistance to oxidation compared to the wild type human and porcine RNase inhibitors, and is stable at low DTT concentrations. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C by non-covalent binding in a 1:1 ratio. RIBOPROTECT Hu-Mut is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.
The RIBOPROTECT Hu RNase Inhibitor is a 50 kDa recombinant human placental protein expressed in Escherichia coli. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C. RIBOPROTECT Hu is intended for use in applications where the presence of RNases may cause a hazard to RNA quality and experiment results, e.g. in RNA isolation, cDNA synthesis, RT-PCR, in vitro transcription and translation, or RNase-free monoclonal antibody preparation. It is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.