RT32L-Reverse-Transcriptase-LYO- TRANSCRIPTME

    TRANSCRIPTME LYO Reverse Transcriptase (RT32L)

    TRANSCRIPTME LYO Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template. TRANSCRIPTME LYO Reverse Transcriptase has increased thermal stability, which allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ –› 5’ exonuclease and reduced RNase H activity, which improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNA synthesis up to 7 kb long.
    RT32L, RT32L-100

    TRANSCRIPTME Reverse Transcriptase (RT32)

    TRANSCRIPTME is a modified, recombinant form of the Reverse Transcriptase from Moloney Murine Leukemia Virus (M-MuLV) purified from Escherichia coli. TRANSCRIPTME Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template.

    RT32, RT32-020, RT32-100

    PCR Anti-inhibitor RP51

    PCR Anti-inhibitor (RP50)

    PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins, which counteracts substances inhibiting the PCR reaction. The addition of PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originated in a DNA template isolation.

    RP50, RP51

    IPTG (B35) – To be discontinued

    IPTG (isopropyl-β-D-1-thiogalactopyranoside) is a chemical analog of lactose, which is not hydrolysed by β-galactosidase.
    It is used during the expression of recombinant protein in a Tabor-Studier as an inducer of the lac promoter. IPTG is also used in combination with X-Gal in the screening of recombinant clones of E. coli based on the known plasmid pUC18/19 system for selecting the white/blue colonies.
    B35,  B35, B325,  B325,

    BSA EN17

    BSA (Bovine Serum Albumin) Fraction V (EN17) – To be discontinued

    BSA (Bovine Serum Albumin, Fraction V) is a protein commonly used in biochemistry and molecular biology. It is a neutral, non-reactive protein, not disturbing to the majority of the reaction, ideal for stabilizing enzymes to increase the efficiency of PCR reaction and preventing the adhesion of enzymes.

    EN17, EN17-010, EN17-100

    UDGase

    Uracil DNA Glycosylase (UDG) (EN19)

    E. coli Uracil DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. The enzyme shows no activity on RNA or oligonucleotides.
    EN19, EN19-050, EN19-250