TRANSCRIPTME LYO Reverse Transcriptase (RT32L)

TRANSCRIPTME LYO Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template. TRANSCRIPTME LYO Reverse Transcriptase has increased thermal stability, which allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ –› 5’ exonuclease and reduced RNase H activity, which improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNA synthesis up to 7 kb long.

TRANSCRIPTME Reverse Transcriptase (RT32)

TRANSCRIPTME is a modified, recombinant form of the Reverse Transcriptase from Moloney Murine Leukemia Virus (M-MuLV) purified from Escherichia coli. TRANSCRIPTME Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template.

PCR Anti-inhibitor (RP50)

PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins, which counteracts substances inhibiting the PCR reaction. The addition of PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originated in a DNA template isolation.

Uracil DNA Glycosylase (UDG) (EN19)

E. coli Uracil DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. The enzyme shows no activity on RNA or oligonucleotides.