Proteins & Enzymes

    TaqNova Stoffel

    TaqNova Stoffel DNA Polymerase (RP810)

    TaqNova Stoffel DNA Polymerase is an universal and easy-to-use DNA polymerase, that works rapidly and effectively in various PCR conditions. The enzyme catalyses DNA synthesis in a 5’→3’ directions, it does not show a 3’→5’ and 5’→3’ exonuclease activity.  
    RP810

    UDGase

    Uracil DNA Glycosylase (UDG) (EN19)

    E. coli Uracil DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. The enzyme shows no activity on RNA or oligonucleotides.
    EN19, EN19-050, EN19-250

    TaqNova DNA Polymerase

    TaqNova DNA Polymerase (RP7A)

    TaqNova DNA Polymerase suited to a wide range of applications, fast and very efficient; universal and easy-to-use; half-life of the enzyme is 45 minutes at 95°C; shows 5’→3’ exonuclease activity; does not have 3’→5’ exonuclease activity; adds A on the 3’ ends.
    RP7, RP702, RP702A, RP705, RP705A, RP710, RP710A, RP725, RP725A

    Phi29 DNA Polymerase EN20

    phi29 Polymerase (EN20)

    phi29 Polymerase is a highly processive recombinant polymerase with exceptional strand displacement activity, therefore allows very efficient isothermal DNA amplification. The enzyme is capable of amplification of up to 70 thousands base insertions per binding event. The phi 29 polymerase possesses a 3’→5’ proofreading exonuclease activity, acting preferentially on ssDNA or RNA. Above all, extremely high yields of amplified DNA can be obtained even from minute amounts of template. Find out more about our Polymerases.
    EN20-10, EN20-50

    RNase A

    RNase A (RP145)

    The Ribonuclease A (RNase A) is a 13.7 kDa (monomer) endoribonuclease isolated from bovine pancreas, which selectively cleaves single-stranded RNA 3’ next to pyrimidine residues (cytosine, uracil). It degrades RNA to cyclic nucleotide monophosphates to 5’- OH and 2’-, 3’-cyclic monophosphate. The enzyme exhibits no endonuclease or exonuclease activity towards DNA substrates. The RNase A is used to remove RNA during the isolation procedures of plasmid and genomic DNA.
    RP147, RP145

    RNase H

    RNase H (RT34)

    RNase H is a 18.9 kDa recombinant endoribonuclease purified from an Escherichia coli strain. The enzyme hydrolyses specifically the phosphodiester bonds of RNA hybridized to DNA and produces 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA. The enzymes does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as RNA bonded to the cDNA template may prevent binding of the amplification primers in a PCR reaction. RNase treatment is often necessary when amplifying longer, full-length cDNA targets. In addition, this enzymes is useful for the removal of poly(A) tails on mRNAs after hybridization with oligo(dT) and also for the site-specific enzymatic cleavage of RNA.

    RT34, RT34-025, RT34-050

    Tth DNA Ligase EN13

    Tth DNA Ligase (EN13)

    Tth DNA Ligase catalyzes the NAD-dependent formation of phosphodiester bonds between adjacent 3’-hydroxyl and 5’-phosphate termini in double stranded DNA. It is not active against single stranded DNA or RNA and blunt ended DNA.

    EN13, EN13-025, EN13-250

    Quick Ligase EN12

    Quick Ligase (EN12)

    ATP-dependent recombinant T4 DNA ligase for efficient ligation of cohesive and or blunt end DNA fragments in 5 and 15 minutes respectively.
    EN12, EN12-050, EN12-150

    T4 DNA Ligase EN11

    T4 DNA Ligase (EN11)

    ATP-dependent recombinant enzyme used for molecular cloning, site-directed mutagenesis, nick repair in duplex DNA, RNA or DNA/RNA hybrids, Ligation Mediated PCR; concentration 5 U/μl
    EN11, EN11-050, EN11-250