Proteins & Enzymes

    Riboprotect LYO

    RIBOPROTECT Hu RNase Inhibitor Lyo-Ready (RT35L) NEW

    The RIBOPROTECT Hu RNase Inhibitor Lyo-Ready is a thermally-resistant RNase Inhibitor in a glycerol-free version, dedicated for lyophilization. The perfect solution for lyophilized qPCR mixes development, RIBOPROTECT remains highly active after lyophilization and is compatible with various additives (see pictures below). Efficiently inhibits ribonuclease activity of common RNases as RNase A, RNase B, RNase C.
    RT35, RT35-020, RT35-100, RT35L, RT35L-020, RT35L-100

    Proteinase K Molecular Biology Grade

    PROTEINASE K Molecular Biology Grade

    Proteinase K Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad spectrum endopeptidase with a very high specific activity. Proteinase K Molecular Biology Grade is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS. As a result, this enzyme is widely using for digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K Molecular Biology Grade is free of exonucleases, endonucleases, and ribonucleases. Also check our Proteinase K NGS Grade
    RP100, RP100B, RP101B, RP102B, RP103B-5, RP103B-10, RP103B-50, RP103B, RP103B-C, RP107B, RP107B-1, RP107B-5

    RIBOPROTECT RNase Inhibitor RT35. Inhibit ribonuclease activity

    RIBOPROTECT Hu RNase Inhibitor (RT35)

    The RIBOPROTECT Hu RNase Inhibitor is a 50 kDa recombinant human placental protein expressed in Escherichia coli. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C, and protects RNA integrity from degradation. RIBOPROTECT Hu is intended for use in applications where the presence of RNases may cause a hazard to RNA quality and experiment results, e.g. in RNA isolation, cDNA synthesis, RT-PCR, in vitro transcription and translation, or RNase-free monoclonal antibody preparation. It is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.
    TaqNova HS DNA polymerase

    TaqNovaHS DNA Polymerase (RP9)

    TaqNovaHS DNA Polymerase is a mixture of thermostable Taq DNA polymerase and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity (for Hot-Start PCR technique); high PCR specificity with minimal optimization; fast 3-minute enzyme activation time; very efficient

    RP902, RP902A, RP905, RP905A, RP910, RP910A, RP925, RP925A

    RT32L-Reverse-Transcriptase-LYO- TRANSCRIPTME

    TRANSCRIPTME LYO Reverse Transcriptase (RT32L)

    TRANSCRIPTME LYO Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template. TRANSCRIPTME LYO Reverse Transcriptase has increased thermal stability, which allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ –› 5’ exonuclease and reduced RNase H activity, which improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNA synthesis up to 7 kb long.
    RT32L, RT32L-100

    TRANSCRIPTME Reverse Transcriptase (RT32)

    TRANSCRIPTME is a modified, recombinant form of the Reverse Transcriptase from Moloney Murine Leukemia Virus (M-MuLV) purified from Escherichia coli. TRANSCRIPTME Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template.

    RT32, RT32-020, RT32-100

    PCR Anti-inhibitor RP51

    PCR Anti-inhibitor (RP50)

    PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins, which counteracts substances inhibiting the PCR reaction. The addition of PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originated in a DNA template isolation.

    RP50, RP51

    Proteinase K NGS Grade

    PROTEINASE K NGS Grade

    Proteinase K NGS Grade  is a broad spectrum endopeptidase with very high specific activity. Proteinase K NGS Grade is developed for most demanding applications. Additional purification technology results in its significantly increased solubility (2.5 fold), increased specific activity, and remarkable purity with DNA content ≤0.1 pg/mg. Product  is available as specially formulated for smooth aliquoting powder or convenient for direct use liquid. Proteinase K NGS Grade is free of exonucleases, endonucleases and ribonucleases. Also check our PROTEINASE K Molecular Biology Grade
    RP100, RP100N, RP101N, RP102N, RP103N, RP107B

    DNaseMe dsDNAse

    DNaseMe, dsDNase (EN33) – NEW

    DNaseMe is a 42.8 kDa recombinant endonuclease, derived from marine amphipods, expressed in Pichia pastoris. The enzyme displays high specific activity towards double-stranded DNA leaving single-stranded DNA or RNA undamaged in standard conditions. DNaseMe is highly active in a broad spectrum of temperatures, buffer conditions and pH. The specific activity is similar to bovine DNase I however, DNaseMe is characterized by higher stability in demanding reaction and storage conditions (e.g. high salt and detergent containing buffers, elevated temperature). These features make DNaseMe extremely useful for rapid and “RNA safe” degradation of genomic DNA, where absence of ribonucleases is critical to maintain the integrity of RNA.
    EN33, EN33-050, EN33-250, EN33-S

    TaqNova DNA free

    TaqNova DNA-free Polymerase (RP10)

    TaqNova DNA-free Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The TaqNova DNA-free Polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR condition. The enzyme catalyzes DNA synthesis in a 5’ → 3’ direction, shows no 3’ → 5’ exonuclease activity, but has a 5’ → 3’ exonuclease activity.
    RP10, RP1002, RP1002-S, RP1010

    Saltonase HL-Nuclease

    Saltonase, HL-Nuclease (EN32) – NEW

    Saltonase, (HL-Nuclease)  is a cold-active, heat-labile recombinant endonuclease produced in E.coli. Saltonase originates from psychrophilic bacteria and effectively digests all types of DNA and RNA substrates in different buffer conditions and a broad range of temperatures. It is very active in demanding conditions, including low temperatures and environment with high salt content. These features make Saltonase extremely useful for removing undesired nucleic acids contamination during purification of proteins in laboratory and manufacturing workflows.
    EN32, EN32-050, EN32-250, EN32-S

    Masterase HL-Nuclease

    Masterase, HL-dsDNase (EN31) – NEW

    Masterase, HL-dsDNase is a 43.3 kDa heat-labile recombinant endonuclease, derived from a cold water eukaryotic organism, expressed in Pichia pastoris. The enzyme displays high specific activity towards double-stranded DNA leaving single-stranded DNA or RNA undamaged in standard conditions. Masterase can be easily inactivated by heat treatment in moderate temperatures. It is intended for applications where the presence of dsDNA influences experiments’ results in thermo-sensitive applications and it is extremely useful for rapid and safe purification of RNA or proteins samples from contaminating DNA.
    EN31, EN31-025, EN31-050, EN31-S, EN31-HC