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2x PCR Hypernova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR Hypernova-RED solution contains a reaction buffer, magnesium chloride, dNTPs, Hypernova polymerase isolated from Pyrococcus woesei and enzymes increasing amplification efficiency. In order to set up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water.
48,00 € NET(59,04 € GROSS)
BSA (Bovine Serum Albumin, Fraction V) is a protein commonly used in biochemistry and molecular biology. It is a neutral, non-reactive protein, not disturbing to the majority of the reaction, ideal for stabilizing enzymes to increase the efficiency of PCR reaction and preventing the adhesion of enzymes.
50,00 € NET(61,50 € GROSS)
The Hypernova DNA Polymerase is a recombinant, thermostable and proofreading Pwo DNA polymerase, originally isolated from the hyperthermophilic archaeon Pyrococcus woesei. The enzyme can generate very long amplicons (up to 10 kbp).
The polymerase is recommended for the multiplex PCR as it works well in a wide range of Mg2+, salt concentration and pH. It is also recommended for the amplification of difficult templates (regions abundant in GC, palindromes and multiple repeats).
42,00 € NET(51,66 € GROSS)
Hypernova-RED DNA polymerase is a variation of Hypernova DNA polymerase containing inner, red dye. Use of the Hypernova-RED DNA polymerase decreases the risk of error occurrence during preparation of reactions e.g. omission of the polymerase or mixing inaccurate reagents.
Hypernova DNA polymerase catalyzes DNA replication reaction at 72°C. The half-life of the polymerase at 95°C is over 8 hours. It shows 3’→ 5’ exonuclease activity (proofreading activity). Any 5’→ 3’ exonuclease activity increases stability of the PCR products. It leaves blunt-ended, 3’ endings (important in molecular cloning) and allows to obtain a wide range of PCR products’ sizes (up to 10 kb).
48,00 € NET(59,04 € GROSS)
PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins, which counteracts substances inhibiting the PCR reaction. The addition of PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originated in a DNA template isolation.
52,00 € NET(63,96 € GROSS)
Phi29 Polymerase is highly processive recombinant polymerase with exceptional strand displacement activity, which allows for highly efficient isothermal DNA amplification. The enzyme is capable of up to 70 thousands base insertions per binding event. The polymerase also possesses a 3’→5’ proofreading exonuclease activity acting preferentially on ssDNA or RNA. Extremely high yields of amplified DNA can be obtained even from minute amounts of template.
90,00 € NET(110,70 € GROSS)
30,00 € NET(36,90 € GROSS)
95,00 € NET(116,85 € GROSS)
125,00 € NET(153,75 € GROSS)
66,00 € NET(81,18 € GROSS)
The RIBOPROTECT Hu-Mut is a genetically modified human placental RNase inhibitor expressed in Escherichia coli. The RIBOPROTECT Hu-Mut has significantly improved resistance to oxidation compared to the wild type human and porcine RNase inhibitors, and is stable at low DTT concentrations. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C by non-covalent binding in a 1:1 ratio. RIBOPROTECT Hu-Mut is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.
79,00 € NET(97,17 € GROSS)
78,00 € NET(95,94 € GROSS)