Proteins & Enzymes

Proteins & Enzymes

Showing 1–12 of 23 results

    2x PCR Hypernova-RED PCR Master Mix (RP85)

    2x PCR Hypernova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR Hypernova-RED solution contains a reaction buffer, magnesium chloride, dNTPs, Hypernova polymerase isolated from Pyrococcus woesei and enzymes increasing amplification efficiency. In order to set up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water.

    RP85, RP85-10

    48,00 € NET

    (59,04 € GROSS)

    BSA (Bovine Serum Albumin) Fraction V (EN17)

    BSA (Bovine Serum Albumin, Fraction V) is a protein commonly used in biochemistry and molecular biology. It is a neutral, non-reactive protein, not disturbing to the majority of the reaction, ideal for stabilizing enzymes to increase the efficiency of PCR reaction and preventing the adhesion of enzymes.

    EN17, EN17-010, EN17-100

    50,00 € NET

    (61,50 € GROSS)

    Hypernova DNA Polymerase

    The Hypernova DNA Polymerase is a recombinant, thermostable and proofreading Pwo DNA polymerase, originally isolated from the hyperthermophilic archaeon Pyrococcus woesei. The enzyme can generate very long amplicons (up to 10 kbp).

    The polymerase is recommended for the multiplex PCR as it works well in a wide range of Mg2+, salt concentration and pH. It is also recommended for the amplification of difficult templates (regions abundant in GC, palindromes and multiple repeats).

    RP232, RP235

    55,00 € NET

    (67,65 € GROSS)

    Hypernova-RED DNA Polymerase

    Hypernova-RED DNA polymerase is a variation of Hypernova DNA polymerase containing inner, red dye. Use of the Hypernova-RED DNA polymerase decreases the risk of error occurrence during preparation of reactions e.g. omission of the polymerase or mixing inaccurate reagents.

    Hypernova DNA polymerase catalyzes DNA replication reaction at 72°C. The half-life of the polymerase at 95°C is over 8 hours. It shows 3’→ 5’ exonuclease activity (proofreading activity). Any 5’→ 3’ exonuclease activity increases stability of the PCR products. It leaves blunt-ended, 3’ endings (important in molecular cloning) and allows to obtain a wide range of PCR products’ sizes (up to 10 kb).

    RP232R, RP235R

    48,00 € NET

    (59,04 € GROSS)

    PCR Anti-inhibitor (RP50)

    PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins, which counteracts substances inhibiting the PCR reaction. The addition of PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originated in a DNA template isolation.

    RP50, RP51

    52,00 € NET

    (63,96 € GROSS)
    phi29 polymerase

    phi29 Polymerase (EN20)

    phi29 Polymerase is a highly processive recombinant polymerase with exceptional strand displacement activity, therefore allows very efficient isothermal DNA amplification. The enzyme is capable of amplification of up to 70 thousands base insertions per binding event. The phi 29 polymerase possesses a 3’→5’ proofreading exonuclease activity, acting preferentially on ssDNA or RNA. Above all, extremely high yields of amplified DNA can be obtained even from minute amounts of template. Find out more about our Polymerases.
    EN20-10, EN20-50

    90,00 € NET

    (110,70 € GROSS)
    Proteinase K NGS Grade

    PROTEINASE K Molecular Biology Grade

    Proteinase K from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad spectrum endopeptidase with a very high specific activity. Proteinase K Molecular Biology Grade is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS. As a result, Proteinase K is widely using for digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K Molecular Biology Grade is free of exonucleases, endonucleases, and ribonucleases. Also check our Proteinase K NGS Grade
    RP100, RP100B, RP101B, RP102B, RP103B-5, RP103B-10, RP103B-50, RP103B, RP103B-C, RP107B, RP107B-1, RP107B-5

    30,00 € NET

    (36,90 € GROSS)
    Proteinase K NGS Grade

    PROTEINASE K NGS Grade

    Proteinase K NGS Grade  is a broad spectrum endopeptidase with very high specific activity. Proteinase K NGS Grade is developed for most demanding applications. Additional purification technology results in its significantly increased solubility (2.5 fold), increased specific activity, and remarkable purity with DNA content ≤0.1 pg/mg. Product  is available as specially formulated for smooth aliquoting powder or convenient for direct use liquid. Proteinase K NGS Grade is free of exonucleases, endonucleases and ribonucleases. Also check our Proteinase K MB Grade
    RP100, RP100N, RP101N, RP102N, RP103N, RP107B

    95,00 € NET

    (116,85 € GROSS)

    Quick Ligase (EN12)

    ATP-dependent recombinant T4 DNA ligase for efficient ligation of cohesive and or blunt end DNA fragments in 5 and 15 minutes respectively.
    EN12, EN12-050, EN12-150

    125,00 € NET

    (153,75 € GROSS)

    RIBOPROTECT Hu RNase Inhibitor (RT35)

    The RIBOPROTECT Hu RNase Inhibitor is a 50 kDa recombinant human placental protein expressed in Escherichia coli. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C. RIBOPROTECT Hu is intended for use in applications where the presence of RNases may cause a hazard to RNA quality and experiment results, e.g. in RNA isolation, cDNA synthesis, RT-PCR, in vitro transcription and translation, or RNase-free monoclonal antibody preparation. It is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.

    66,00 € NET

    (81,18 € GROSS)
    Riboprotect Hu-Mut RNase Inhibitor

    RIBOPROTECT Hu-Mut RNase Inhibitor (RT36)

    The RIBOPROTECT Hu-Mut is a genetically modified human placental RNase inhibitor expressed in Escherichia coli. The RIBOPROTECT Hu-Mut has significantly improved resistance to oxidation compared to the wild type human and porcine RNase inhibitors, and is stable at low DTT concentrations. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C by non-covalent binding in a 1:1 ratio. RIBOPROTECT Hu-Mut is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.
    RT36, RT36-020, RT36-100

    79,00 € NET

    (97,17 € GROSS)
    RNase A

    RNase A (RP145)

    The Ribonuclease A (RNase A) is a 13.7 kDa (monomer) endoribonuclease isolated from bovine pancreas, which selectively cleaves single-stranded RNA 3’ next to pyrimidine residues (cytosine, uracil). It degrades RNA to cyclic nucleotide monophosphates to 5’- OH and 2’-, 3’-cyclic monophosphate. The enzyme exhibits no endonuclease or exonuclease activity towards DNA substrates. The RNase A is used to remove RNA during the isolation procedures of plasmid and genomic DNA.
    RP147, RP145

    78,00 € NET

    (95,94 € GROSS)