RIBOPROTECT Hu RNase Inhibitor (RT35)

The RIBOPROTECT Hu RNase Inhibitor is a 50 kDa recombinant human placental protein expressed in Escherichia coli. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C, and protects RNA integrity from degradation. RIBOPROTECT Hu is used in applications where the presence of RNases causes a substantial hazard in receiving good quality, trustworthy, and reproducible data, e.g. in RNA isolation, cDNA synthesis, RT-PCR, in vitro transcription and translation, or RNase-free monoclonal antibody preparation. It is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.

PROTEINASE K Molecular Biology Grade

Proteinase K MB Grade - lyophilized or liquid version tailored to your application.

Boost the efficiency of protein digestion with custom-made enzymes. BLIRT - expert in Proteinase K production for over 15 years. Certified manufacturing process ISO 13485 resulting in the highest quality product with a very high batch-to-batch consistency.

• Short lead time for bulk quantities.
• Portioning service with the possibility of white labeling.
• High throughput for aliquoting of the liquid form (up to several million samples monthly).

 

TaqNovaHS DNA Polymerase (RP9)

TaqNovaHS DNA Polymerase is a mixture of thermostable Taq DNA polymerase and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity (for Hot-Start PCR technique); high PCR specificity with minimal optimization; fast 3-minute enzyme activation time; very efficient

TRANSCRIPTME LYO Reverse Transcriptase (RT32L)

TRANSCRIPTME LYO Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template. TRANSCRIPTME LYO Reverse Transcriptase has increased thermal stability, which allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ –› 5’ exonuclease and reduced RNase H activity, which improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNA synthesis up to 7 kb long.

TRANSCRIPTME Reverse Transcriptase (RT32)

TRANSCRIPTME is a modified, recombinant form of the Reverse Transcriptase from Moloney Murine Leukemia Virus (M-MuLV) purified from Escherichia coli. TRANSCRIPTME Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template.

PCR Anti-inhibitor (RP50)

PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins, which counteracts substances inhibiting the PCR reaction. The addition of PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originated in a DNA template isolation.

PROTEINASE K NGS Grade

Proteinase K NGS Grade is a broad spectrum endopeptidase with a very high specific activity developed for most demanding applications. Additional purification technology results in its significantly increased solubility (2.5 fold), increased specific activity, and remarkable purity with DNA content ≤0.1 pg/mg. The product is available as specially formulated for smooth aliquoting lyophilized powder. Proteinase K NGS Grade is free of exonucleases, endonucleases, and ribonucleases. Also, check our PROTEINASE K Molecular Biology Grade

DNaseMe, dsDNase (EN33)

DNaseMe is a 42.8 kDa recombinant endonuclease, derived from marine amphipods, expressed in Pichia pastoris. The enzyme displays high specific activity towards double-stranded DNA leaving single-stranded DNA or RNA undamaged in standard conditions. DNaseMe is highly active in a broad spectrum of temperatures, buffer conditions and pH. The specific activity is similar to bovine DNase I however, DNaseMe is characterized by higher stability in demanding reaction and storage conditions (e.g. high salt and detergent containing buffers, elevated temperature). These features make DNaseMe extremely useful for rapid and “RNA safe” degradation of genomic DNA, where absence of ribonucleases is critical to maintain the integrity of RNA.