PCR & Real-Time PCR

PCR & Real-Time PCR

Showing 1–12 of 27 results

    TaqNova DNA-free Polymerase (RP10)

    TaqNova DNA-free Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The TaqNova DNA-free Polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR condition. The enzyme catalyzes DNA synthesis in a 5’ → 3’ direction, shows no 3’ → 5’ exonuclease activity, but has a 5’ → 3’ exonuclease activity.
    RP10, RP1002, RP1002-S, RP1010

    110,00 € NET

    (135,30 € GROSS)

    AMPLIFYME Probe One-Step Universal RT-qPCR Mix (AM09)

    Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on probe detection technology: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers.
    AM09, AM09-100, AM09-500

    0,00 € NET

    (0,00 € GROSS)

    AMPLIFYME Probe One-Step No-ROX RT-qPCR Mix (AM08)

    Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on probe detection technology: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers.
    AM08, AM08-100, AM08-500

    0,00 € NET

    (0,00 € GROSS)

    AMPLIFYME SG One-Step Universal RT-qPCR Mix (AM07)

    Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on intercalating dsDNA binding dye chemistry: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers.
    AM07, AM07-100, AM07-500

    0,00 € NET

    (0,00 € GROSS)

    AMPLIFYME SG One-Step No-ROX RT-qPCR Mix (AM06)

    Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on intercalating dsDNA binding dye chemistry: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers.
    AM06, AM06-100, AM06-500

    0,00 € NET

    (0,00 € GROSS)
    TaqNova Stoffel DNA Polymerase

    TaqNova Stoffel DNA Polymerase (RP810)

    TaqNova Stoffel DNA Polymerase is an universal and easy-to-use DNA polymerase, that works rapidly and effectively in various PCR conditions. The enzyme catalyses DNA synthesis in a 5’→3’ directions, it does not show a 3’→5’ and 5’→3’ exonuclease activity.  
    RP810

    142,00 € NET

    (174,66 € GROSS)

    PCR Anti-inhibitor (RP50)

    PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins, which counteracts substances inhibiting the PCR reaction. The addition of PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originated in a DNA template isolation.

    RP50, RP51

    52,00 € NET

    (63,96 € GROSS)

    5x GC-Additive (RP516)

    5x GC-Additive is an ideal tool for the amplification of GC-rich templates; reduces the number of secondary structures and enables the specific hybridization of primers.
    RP516, RP517

    40,00 € NET

    (49,20 € GROSS)

    2x PCR Hypernova-RED PCR Master Mix (RP85)

    2x PCR Hypernova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR Hypernova-RED solution contains a reaction buffer, magnesium chloride, dNTPs, Hypernova polymerase isolated from Pyrococcus woesei and enzymes increasing amplification efficiency. In order to set up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water.

    RP85, RP85-10

    48,00 € NET

    (59,04 € GROSS)

    Hypernova-RED DNA Polymerase

    Hypernova-RED DNA polymerase is a variation of Hypernova DNA polymerase containing inner, red dye. Use of the Hypernova-RED DNA polymerase decreases the risk of error occurrence during preparation of reactions e.g. omission of the polymerase or mixing inaccurate reagents.

    Hypernova DNA polymerase catalyzes DNA replication reaction at 72°C. The half-life of the polymerase at 95°C is over 8 hours. It shows 3’→ 5’ exonuclease activity (proofreading activity). Any 5’→ 3’ exonuclease activity increases stability of the PCR products. It leaves blunt-ended, 3’ endings (important in molecular cloning) and allows to obtain a wide range of PCR products’ sizes (up to 10 kb).

    RP232R, RP235R

    48,00 € NET

    (59,04 € GROSS)

    Hypernova DNA Polymerase

    The Hypernova DNA Polymerase is a recombinant, thermostable and proofreading Pwo DNA polymerase, originally isolated from the hyperthermophilic archaeon Pyrococcus woesei. The enzyme can generate very long amplicons (up to 10 kbp).

    The polymerase is recommended for the multiplex PCR as it works well in a wide range of Mg2+, salt concentration and pH. It is also recommended for the amplification of difficult templates (regions abundant in GC, palindromes and multiple repeats).

    RP232, RP235

    55,00 € NET

    (67,65 € GROSS)

    dNTPs SET 100 mM (RP675)

    dNTPs SET 100 mM is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates 100 mM of each dNTP (separately: dATP, dCTP, dGTP, dTTP), supplied as lithium salts solutions. (much higher stability) Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.
    RP675

    465,00 € NET

    (571,95 € GROSS)