Molecular Biology Reagents

    TaqNova DNA Polymerase

    TaqNova DNA Polymerase (RP7A)

    TaqNova DNA Polymerase suited to a wide range of applications, fast and very efficient; universal and easy-to-use; half-life of the enzyme is 45 minutes at 95°C; shows 5’→3’ exonuclease activity; does not have 3’→5’ exonuclease activity; adds A on the 3’ ends.
    RP7, RP702, RP702A, RP705, RP705A, RP710, RP710A, RP725, RP725A

    EXTRACTME PLASMID MAXI (EM18)

    The EXTRACTME PLASMID MAXI kit (EM18) and EXTRACTME PLASMID MAXI ENDOTOXIN-FREE kit (EM19) are designed for the efficient purification of high quality plasmid DNA from 200-1000 ml of cultured bacterial cells. The kits are based on anion-exchange resins, allowing extraction of ultrapure, transfectiongrade pDNA, which is highly suited for use in demanding applications. The isolation protocol and buffer formulations were optimized for high isolation efficiency and pDNA purity. The product is intended for research use only.

    EM18, EM18-010, EM18-025

    EXTRACTME PLASMID MIDI (EM16)

    The EXTRACTME PLASMID MIDI kit (EM16) and are designed for ultrapure, transfection-grade plasmid DNA isolation im medium scale (50-300 ml of bacterial culture); yield: 200-600 μg DNA from 100 ml culture; isolation time: 120-130 minutes (with DNA precipitation); centrifugation steps: 6000 x g (no need to have ultracentrifuge); laskyophilized RNase A included; each column contain special modificated anion-exchange resin.

    The product is intended for research use only.

    EM16, EM16-010, EM16-025

    EXTRACTME GENOMIC DNA KIT (EM13)

    The EXTRACTME GENOMIC DNA KIT is designed for a rapid and efficient purification of high quality genomic, mitochondrial, bacterial, parasite or viral DNA from solid tissues, physiological fluids, fresh and frozen blood, human and animal mucosa membrane swabs, semen, hair, rodent tails, insects, bacteria, yeast and cell cultures. The isolation protocol and buffer formulations have been optimized for high isolation efficiency and DNA purity. The product is intended for research use only.
    EM13, EM13-010, EM13-050, EM13-250

    EXTRACTME miRNA KIT (EM12)

    The EXTRACTME miRNA KIT is designed for the rapid and efficient purification of miRNA with possibility of simultaneous purification of large RNA and DNA. High quality miRNA may be purified from 1-10 mg of tissue (fresh or frozen) and 104-106 cultured cells. The isolation protocol, buffers formulations and columns were optimized for high isolation efficiency and purity of miRNA. The product is intended for research use only.

    EM12, EM12-010, EM12-050, EM12-250

    DNA SWAB & SEMEN KIT

    EXTRACTME DNA SWAB &SEMEN KIT (EM06)

    The EXTRACTME DNA SWAB & SEMEN KIT is designed for a rapid and efficient purification of high quality DNA from human and animal mucosa membrane swabs (including buccal, nasal, pharyngeal and vaginal swabs) as well as from semen. The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity. The product is intended for research use only.
    EM06, EM06-010, EM06-050, EM06-250

    EXTRACTME DNA BLOOD KIT (EM05)

    The EXTRACTME DNA BLOOD KIT is designed for a rapid and efficient purification of high quality (genomic, mitochondrial and viral) DNA from whole blood (fresh or frozen, human or mammalian), plasma, serum, buffy coat, lymphocytes and body fluids. The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity. The product is intended for research use only.
    EM05-010, EM05-050, EM05-250

    EXTRACTME DNA TISSUE KIT (EM03)

    The EXTRACTME DNA TISSUE KIT is designed for a rapid and efficient purification of high quality DNA from solid tissues (fresh, frozen, formalin-preserved or paraffin-embedded), physiological fluids, hair, rodent tails, insects and cell cultures. The isolation protocol and buffer formulations have been optimized for high isolation efficiency and DNA purity. The product is intended for research use only.
    EM03, EM03-010, EM03-050, EM03-250

    RNase H

    RNase H (RT34)

    RNase H is a 18.9 kDa recombinant endoribonuclease purified from an Escherichia coli strain. The enzyme hydrolyses specifically the phosphodiester bonds of RNA hybridized to DNA and produces 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA. The enzymes does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as RNA bonded to the cDNA template may prevent binding of the amplification primers in a PCR reaction. RNase treatment is often necessary when amplifying longer, full-length cDNA targets. In addition, this enzymes is useful for the removal of poly(A) tails on mRNAs after hybridization with oligo(dT) and also for the site-specific enzymatic cleavage of RNA.

    RT34, RT34-025, RT34-050

    AMPLIFYME Probe Universal Mix (AM05)

    The AMPLIFYME Probe Universal Mix is a convenient enzyme mixture for fast and reliable qPCR using probes, including TaqMan®, Scorpions® and molecular beacon probes. It is the best choice for your probe based Real-Time PCR assays, including singleplex and multiplex gene expression studies, genotyping experiments or diagnostic assays. Universal - compatible with all types of qPCR instruments. Additional tubes with low and high concentration of ROX are included.
    AM05, AM05-200, AM05-020

    AMPLIFYME Probe No-Rox Mix (AM04)

    Convenient enzyme mixture for fast and reliable qPCR using probes, including TaqMan®, Scorpions® and molecular beacon probes. It is the best choice for your probe based Real-Time PCR assays, including singleplex and multiplex gene expression studies, genotyping experiments or diagnostic assays. Compatible with qPCR instruments that don't need ROX dye.
    AM04, AM04-200, AM04-020

    AMPLIFYME SG RT-PCR Mixes

    AMPLIFYME SG UNIVERSAL Mix (AM02)

    The AMPLIFYME SG Universal Mix is a convenient enzyme mixture for fast and reliable quantitative Real-Time PCR, using SG dsDNA-binding dye. Compatible with all types of qPCR instruments. Additional tubes with low and high concentration of ROX are included.
    AM02, AM02-200, AM02-020