The EXTRACTME PLASMID MAXI kit (EM18) and EXTRACTME PLASMID MAXI ENDOTOXIN-FREE kit (EM19) are designed for the efficient purification of high quality plasmid DNA from 200-1000 ml of cultured bacterial cells. The kits are based on anion-exchange resins, allowing extraction of ultrapure, transfectiongrade pDNA, which is highly suited for use in demanding applications. The isolation protocol and buffer formulations were optimized for high isolation efficiency and pDNA purity. The product is intended for research use only.
The EXTRACTME PLASMID MIDI kit (EM16) and are designed for ultrapure, transfection-grade plasmid DNA isolation im medium scale (50-300 ml of bacterial culture); yield: 200-600 μg DNA from 100 ml culture; isolation time: 120-130 minutes (with DNA precipitation); centrifugation steps: 6000 x g (no need to have ultracentrifuge); laskyophilized RNase A included; each column contain special modificated anion-exchange resin.
The product is intended for research use only.
The EXTRACTME miRNA KIT is designed for the rapid and efficient purification of miRNA with possibility of simultaneous purification of large RNA and DNA. High quality miRNA may be purified from 1-10 mg of tissue (fresh or frozen) and 104-106 cultured cells. The isolation protocol, buffers formulations and columns were optimized for high isolation efficiency and purity of miRNA. The product is intended for research use only.
RNase H is a 18.9 kDa recombinant endoribonuclease purified from an Escherichia coli strain. The enzyme hydrolyses specifically the phosphodiester bonds of RNA hybridized to DNA and produces 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA. The enzymes does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as RNA bonded to the cDNA template may prevent binding of the amplification primers in a PCR reaction. RNase treatment is often necessary when amplifying longer, full-length cDNA targets. In addition, this enzymes is useful for the removal of poly(A) tails on mRNAs after hybridization with oligo(dT) and also for the site-specific enzymatic cleavage of RNA.