Molecular Biology Reagents

    RIBOPROTECT

    RIBOPROTECT Hu RNase Inhibitor (RT35)

    The RIBOPROTECT Hu RNase Inhibitor is a 50 kDa recombinant human placental protein expressed in Escherichia coli. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C, and protects RNA integrity from degradation. RIBOPROTECT Hu is used in applications where the presence of RNases causes a substantial hazard in receiving good quality, trustworthy, and reproducible data, e.g. in RNA isolation, cDNA synthesis, RT-PCR, in vitro transcription and translation, or RNase-free monoclonal antibody preparation. It is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.
    Proteinase K Molecular Biology Grade

    PROTEINASE K Molecular Biology Grade

    Proteinase K MB Grade - lyophilized or liquid version tailored to your application.

    Boost the efficiency of protein digestion with custom-made enzymes. BLIRT - expert in Proteinase K production for over 15 years. Certified manufacturing process ISO 13485 resulting in the highest quality product with a very high batch-to-batch consistency.
    • Short lead time for bulk quantities.
    • Portioning service with the possibility of white labeling.
    • High throughput for aliquoting of the liquid form (up to several million samples monthly).
    RP100, RP100B, RP101B, RP102B, RP103B-5, RP103B-10, RP103B-50, RP103B, RP103B-C, RP107B, RP107B-1, RP107B-5
     
    Riboprotect LYO

    RIBOPROTECT Hu RNase Inhibitor Lyo-Ready (RT35L)

    The RIBOPROTECT Hu RNase Inhibitor Lyo-Ready is a thermally-resistant RNase Inhibitor in a glycerol-free version, dedicated for lyophilization. The perfect solution for lyophilized qPCR mixes development, RIBOPROTECT remains highly active after lyophilization and is compatible with various additives (check the section "Product Characteristics"). Efficiently inhibits ribonuclease activity of common RNases as RNase A, RNase B, RNase C.
    RT35, RT35-020, RT35-100, RT35L, RT35L-020, RT35L-100

    TaqNova HS DNA polymerase

    TaqNovaHS DNA Polymerase (RP9)

    TaqNovaHS DNA Polymerase is a mixture of thermostable Taq DNA polymerase and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity (for Hot-Start PCR technique); high PCR specificity with minimal optimization; fast 3-minute enzyme activation time; very efficient

    RP902, RP902A, RP905, RP905A, RP910, RP910A, RP925, RP925A

    RT32L-Reverse-Transcriptase-LYO- TRANSCRIPTME

    TRANSCRIPTME LYO Reverse Transcriptase (RT32L)

    TRANSCRIPTME LYO Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template. TRANSCRIPTME LYO Reverse Transcriptase has increased thermal stability, which allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ –› 5’ exonuclease and reduced RNase H activity, which improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNA synthesis up to 7 kb long.
    RT32L, RT32L-100

    TRANSCRIPTME Reverse Transcriptase (RT32)

    TRANSCRIPTME is a modified, recombinant form of the Reverse Transcriptase from Moloney Murine Leukemia Virus (M-MuLV) purified from Escherichia coli. TRANSCRIPTME Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template.

    RT32, RT32-020, RT32-100

    AMPLIFYME Probe One-Step Universal RT-qPCR Mix AM09.1

    AMPLIFYME Probe One-Step Universal RT-qPCR Mix (AM09.1) – New version

    AMPLIFYME Probe One-Step Universal RT-qPCR Mix (AM09.1) Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on probe detection technology: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers. Additionally, Mu-MLV Reverse Transcriptase and RNase Inhibitor and ROX solution are included in separate tubes.
    AM09, AM09-100, AM09-500,AM09.1, AM09.1-100, AM09.1-500

    RT-qPCR Mix Probe One-Step No-ROX

    AMPLIFYME Probe One-Step No-ROX RT-qPCR Mix (AM08.1) – New version

    The AMPLIFYME Probe One-Step No-ROX RT-qPCR Mix - created for reproducible and efficient first-strand cDNA synthesis and subsequent Real-Time PCR in a single tube. It is the best choice for your probe based Real-Time PCR assays, including singleplex and multiplex gene expression studies. Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on probe detection technology: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers. Additionally, Mu-MLV Reverse Transcriptase and RNase Inhibitor are included in separate tubes.
    AM08, AM08-100, AM08-500, AM08.1, AM08-100.1, AM08-500.1

    PCR Anti-inhibitor RP51

    PCR Anti-inhibitor (RP50)

    PCR Anti-inhibitor is a carefully composed mixture of alkaline proteins, which counteracts substances inhibiting the PCR reaction. The addition of PCR Anti-inhibitor to a reaction mixture is an ideal way of eliminating inhibitors originated in a DNA template isolation.

    RP50, RP51

    Proteinase K NGS Grade

    PROTEINASE K NGS Grade

    Proteinase K NGS Grade is a broad spectrum endopeptidase with a very high specific activity developed for most demanding applications. Additional purification technology results in its significantly increased solubility (2.5 fold), increased specific activity, and remarkable purity with DNA content ≤0.1 pg/mg. The product is available as specially formulated for smooth aliquoting lyophilized powder. Proteinase K NGS Grade is free of exonucleases, endonucleases, and ribonucleases. Also, check our PROTEINASE K Molecular Biology Grade
    RP100, RP100N, RP101N, RP102N, RP103N, RP107B

    DNaseMe dsDNAse

    DNaseMe, dsDNase (EN33)

    DNaseMe is a 42.8 kDa recombinant endonuclease, derived from marine amphipods, expressed in Pichia pastoris. The enzyme displays high specific activity towards double-stranded DNA leaving single-stranded DNA or RNA undamaged in standard conditions. DNaseMe is highly active in a broad spectrum of temperatures, buffer conditions and pH. The specific activity is similar to bovine DNase I however, DNaseMe is characterized by higher stability in demanding reaction and storage conditions (e.g. high salt and detergent containing buffers, elevated temperature). These features make DNaseMe extremely useful for rapid and “RNA safe” degradation of genomic DNA, where absence of ribonucleases is critical to maintain the integrity of RNA.
    EN33, EN33-050, EN33-250, EN33-S

    TaqNova DNA free

    TaqNova DNA-free Polymerase (RP10)

    TaqNova DNA-free Polymerase is a 94 kDa recombinant, thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications which require DNA synthesis at extremely high temperatures. The TaqNova DNA-free Polymerase is a universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR condition. The enzyme catalyzes DNA synthesis in a 5’ → 3’ direction, shows no 3’ → 5’ exonuclease activity, but has a 5’ → 3’ exonuclease activity.
    RP10, RP1002, RP1002-S, RP1010