Discontinued Molecular Biology Reagents

    AMPLIFYME Probe One-Step Universal RT-qPCR Mix (AM09) -Discontinued

    Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on probe detection technology: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers.
    AM09, AM09-100, AM09-500

    AMPLIFYME Probe One-Step No-ROX RT-qPCR Mix (AM08) -Discontinued

    Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on probe detection technology: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers.
    AM08, AM08-100, AM08-500,

    AMPLIFYME SG One-Step Universal RT-qPCR Mix (AM07) – To be discontinued

    Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on intercalating dsDNA binding dye chemistry: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers.
    AM07, AM07-100, AM07-500

    AMPLIFYME SG One-Step No-ROX RT-qPCR Mix (AM06) – To be discontinued

    Ready-to-use, 2x concentrated Mix contains all ingredients necessary for Real-Time PCR based on intercalating dsDNA binding dye chemistry: hot-start Taq polymerase, dNTPs, specially developed buffer, stabilizers and enhancers.
    AM06, AM06-100, AM06-500

    EasyGenotyping ITS PCR (DY62) – Discontinued

    EasyGenotyping ITS PCR. The aim of this experiment is to use the ribotyping for the intra- and interspecies differentiation of the supplied bacterial strains (DNA from 6 different species, 3 isolates each). The EasyGenotyping ITS PCR kit is designed to perform experiments during two laboratory classes (I - PCR set-up - 1.5-2 hours, II - electrophoresis - 2 hours). We suggest to perform laboratory experiments in six groups of two students.
    DY62, DY625

    EasyPCR HIV+ DNA isolatio Edu Kits

    EasyPCR HIV (+ DNA extraction) (DY25) – To be discontinued

    EasyPCR HIV (+ DNA extraction) Aim: to carry out a genomic DNA isolation and a PCR reaction with the isolated templates in order to detect the mutation in the ccr5 gene which confers resistance to HIV in humans. Set with reagents for DNA extraction.
    DY25, DY255

    EasyPCR XY (+ DNA extraction) (DY10) – To be discontinued

    EasyPCR XY (+ DNA extraction) AIM: The purpose of the exercise is to carry out a genomic DNA isolation from buccal swabs and a PCR reaction with the isolated DNA templates in order to determine human sex using PCR. The procedure is based on the amplification of a human amelogenin gene fragment. This gene is shorter by 189bp on chromosome Y as compared with chromosome X, which allows for the determination of human sex by means of PCR. The expected PCR products are 977bp and 788bp long for males, or only 977bp for females
    DY10, DY105

    EasyPCR XY (DY10A/DY105A) – To be discontinued

    EasyPCR XY AIM: The purpose of the exercise is to carry a PCR reaction with the DNA templates in order to determine human sex using PCR. The procedure is based on the amplification of a human amelogenin gene fragment. This gene is shorter by 189bp on chromosome Y as compared with chromosome X, which allows for the determination of human sex by means of PCR. The expected PCR products are 977bp and 788bp long for males, or only 977bp for females.
    DY10A, DY105A

    EasyPCR I

    EasyPCR I (DY45) – Discontinued

    EasyPCRI Kit -aim: presentation of an influence of changing reaction components onto the reaction efficiency. The following will be tested: composition of the reaction buffer, magnesium and nucleotides (dNTPs) concentration and the amount of template. The EasyPCRI kit is designed to perform experiments during two laboratory classes (I - PCR set-up - 2 hours; II - electrophoresis - 2 hours). We suggest to perform laboratory experiments in six groups of two students.
    DY45, DY455

    EXTRACTME DNA TISSUE PLUS KIT (EM04) – To be discontinued

    The EXTRACTME DNA TISSUE PLUS KIT is designed for a rapid and efficient purification of high quality DNA from solid tissues, physiological fluids, hair, rodent tails, insects and cell cultures. Kit includes additional bead-beating tubes with ceramic filling for tissue homogenization. The product is intended for research use only.
    EM04, EM04-010, EM04-050, EM04-250

    5x GC-Additive (RP516) – Discontinued

    5x GC-Additive is an ideal tool for the amplification of GC-rich templates; reduces the number of secondary structures and enables the specific hybridization of primers.
    RP516, RP517

    2x PCR Hypernova-RED PCR Master Mix (RP85) – Discontinued

    2x PCR Hypernova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR Hypernova-RED solution contains a reaction buffer, magnesium chloride, dNTPs, Hypernova polymerase isolated from Pyrococcus woesei and enzymes increasing amplification efficiency. In order to set up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water.

    RP85, RP85-10