Discontinued Proteins & Enzymes

    TEV Protease

    TEV Protease (RP17) – Discontinued

    Recombinant TEV Protease is a highly site-specific cysteine protease, which is found in the Tobacco Etch Virus. Due to its sequence specificity, the TEV protease is a very powerful reagent for the removal of fusion tags from recombinant proteins after protein purification. TEV Protease specifically recognizes a seven amino acid sequence of the general form Glu-X-X-Tyr-X-Gln↓ (Gly/Ser). Most commonly Glu-Asn-Leu-Tyr-Phe-Gln↓ Gly, and cleaves between glutamine and glycine or serine.
    RP171, RP172

    IPTG (B35) – To be discontinued

    IPTG (isopropyl-β-D-1-thiogalactopyranoside) is a chemical analog of lactose, which is not hydrolysed by β-galactosidase.
    It is used during the expression of recombinant protein in a Tabor-Studier as an inducer of the lac promoter. IPTG is also used in combination with X-Gal in the screening of recombinant clones of E. coli based on the known plasmid pUC18/19 system for selecting the white/blue colonies.
    B35,  B35, B325,  B325,

    TaqNova HS DNA polymerase

    TaqNovaHS DNA Polymerase ( 2U/μl ) – Discontinued

    TaqNovaHS DNA Polymerase is amixture of thermostable Taq DNA polymerase and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity (for Hot-Start PCR technique); high PCR specificity with minimal optimization; fast 3-minute enzyme activation time; very efficient

    RP902,  RP905,  RP910,  RP925,

    TaqNova DNA Polymerase

    TaqNova DNA Polymerase ( 2U/μl ) – Discontinued

    TaqNova DNA Polymerase suited to a wide range of applications, fast and very efficient; universal and easy-to-use; half-life of the enzyme is 45 minutes at 95°C; shows 5’→3’ exonuclease activity; does not have 3’→5’ exonuclease activity; adds A on the 3’ ends.
    RP7,  RP702, RP705, RP710,  RP725,

    RIBOPROTECT Hu-Mut RNase Inhibitor RT36

    RIBOPROTECT Hu-Mut RNase Inhibitor (RT36) – Discontinued

    The RIBOPROTECT Hu-Mut is a genetically modified human placental RNase inhibitor expressed in Escherichia coli. The RIBOPROTECT Hu-Mut has significantly improved resistance to oxidation compared to the wild type human and porcine RNase inhibitors, and is stable at low DTT concentrations. It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C by non-covalent binding in a 1:1 ratio. RIBOPROTECT Hu-Mut is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases.
    RT36, RT36-020, RT36-100

    BSA EN17

    BSA (Bovine Serum Albumin) Fraction V (EN17) – To be discontinued

    BSA (Bovine Serum Albumin, Fraction V) is a protein commonly used in biochemistry and molecular biology. It is a neutral, non-reactive protein, not disturbing to the majority of the reaction, ideal for stabilizing enzymes to increase the efficiency of PCR reaction and preventing the adhesion of enzymes.

    EN17, EN17-010, EN17-100

    2x PCR Hypernova-RED PCR Master Mix (RP85) – Discontinued

    2x PCR Hypernova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR Hypernova-RED solution contains a reaction buffer, magnesium chloride, dNTPs, Hypernova polymerase isolated from Pyrococcus woesei and enzymes increasing amplification efficiency. In order to set up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water.

    RP85, RP85-10

    Hypernova-RED DNA Polymerase – Discontinued

    Hypernova-RED DNA polymerase is a variation of Hypernova DNA polymerase containing inner, red dye. Use of the Hypernova-RED DNA polymerase decreases the risk of error occurrence during preparation of reactions e.g. omission of the polymerase or mixing inaccurate reagents.

    Hypernova DNA polymerase catalyzes DNA replication reaction at 72°C. The half-life of the polymerase at 95°C is over 8 hours. It shows 3’→ 5’ exonuclease activity (proofreading activity). Any 5’→ 3’ exonuclease activity increases stability of the PCR products. It leaves blunt-ended, 3’ endings (important in molecular cloning) and allows to obtain a wide range of PCR products’ sizes (up to 10 kb).

    RP232R, RP235R

    Hypernova DNA Polymerase

    Hypernova DNA Polymerase – to be discontinued

    The Hypernova DNA Polymerase is a recombinant, thermostable and proofreading Pwo DNA polymerase, originally isolated from the hyperthermophilic archaeon Pyrococcus woesei. The enzyme can generate very long amplicons (up to 10 kbp).

    The polymerase is recommended for the multiplex PCR as it works well in a wide range of Mg2+, salt concentration and pH. It is also recommended for the amplification of difficult templates (regions abundant in GC, palindromes and multiple repeats).

    RP232, RP235