Discontinued Products

    EasyPCR XY (DY10A/DY105A) – To be discontinued

    EasyPCR XY AIM: The purpose of the exercise is to carry a PCR reaction with the DNA templates in order to determine human sex using PCR. The procedure is based on the amplification of a human amelogenin gene fragment. This gene is shorter by 189bp on chromosome Y as compared with chromosome X, which allows for the determination of human sex by means of PCR. The expected PCR products are 977bp and 788bp long for males, or only 977bp for females.
    DY10A, DY105A

    EasyPCR I

    EasyPCR I (DY45) – Discontinued

    EasyPCRI Kit -aim: presentation of an influence of changing reaction components onto the reaction efficiency. The following will be tested: composition of the reaction buffer, magnesium and nucleotides (dNTPs) concentration and the amount of template. The EasyPCRI kit is designed to perform experiments during two laboratory classes (I - PCR set-up - 2 hours; II - electrophoresis - 2 hours). We suggest to perform laboratory experiments in six groups of two students.
    DY45, DY455

    EXTRACTME DNA TISSUE PLUS KIT (EM04) – To be discontinued

    The EXTRACTME DNA TISSUE PLUS KIT is designed for a rapid and efficient purification of high quality DNA from solid tissues, physiological fluids, hair, rodent tails, insects and cell cultures. Kit includes additional bead-beating tubes with ceramic filling for tissue homogenization. The product is intended for research use only.
    EM04, EM04-010, EM04-050, EM04-250

    BSA EN17

    BSA (Bovine Serum Albumin) Fraction V (EN17) – To be discontinued

    BSA (Bovine Serum Albumin, Fraction V) is a protein commonly used in biochemistry and molecular biology. It is a neutral, non-reactive protein, not disturbing to the majority of the reaction, ideal for stabilizing enzymes to increase the efficiency of PCR reaction and preventing the adhesion of enzymes.

    EN17, EN17-010, EN17-100

    5x GC-Additive (RP516) – Discontinued

    5x GC-Additive is an ideal tool for the amplification of GC-rich templates; reduces the number of secondary structures and enables the specific hybridization of primers.
    RP516, RP517

    2x PCR Hypernova-RED PCR Master Mix (RP85) – Discontinued

    2x PCR Hypernova-RED is a 2x concentrated, ready-to-use PCR master mix which facilitates an easy and rapid PCR reaction set up. The 2x PCR Hypernova-RED solution contains a reaction buffer, magnesium chloride, dNTPs, Hypernova polymerase isolated from Pyrococcus woesei and enzymes increasing amplification efficiency. In order to set up the PCR reaction, all that needs adding to the master mix is the template, the primer set and water.

    RP85, RP85-10

    Hypernova-RED DNA Polymerase – Discontinued

    Hypernova-RED DNA polymerase is a variation of Hypernova DNA polymerase containing inner, red dye. Use of the Hypernova-RED DNA polymerase decreases the risk of error occurrence during preparation of reactions e.g. omission of the polymerase or mixing inaccurate reagents.

    Hypernova DNA polymerase catalyzes DNA replication reaction at 72°C. The half-life of the polymerase at 95°C is over 8 hours. It shows 3’→ 5’ exonuclease activity (proofreading activity). Any 5’→ 3’ exonuclease activity increases stability of the PCR products. It leaves blunt-ended, 3’ endings (important in molecular cloning) and allows to obtain a wide range of PCR products’ sizes (up to 10 kb).

    RP232R, RP235R

    Hypernova DNA Polymerase

    Hypernova DNA Polymerase – to be discontinued

    The Hypernova DNA Polymerase is a recombinant, thermostable and proofreading Pwo DNA polymerase, originally isolated from the hyperthermophilic archaeon Pyrococcus woesei. The enzyme can generate very long amplicons (up to 10 kbp).

    The polymerase is recommended for the multiplex PCR as it works well in a wide range of Mg2+, salt concentration and pH. It is also recommended for the amplification of difficult templates (regions abundant in GC, palindromes and multiple repeats).

    RP232, RP235

    dNTPs SET 100 mM (RP675) – To be discontinued

    dNTPs SET 100 mM is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates 100 mM of each dNTP (separately: dATP, dCTP, dGTP, dTTP), supplied as lithium salts solutions. (much higher stability) Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.
    RP675

    dNTPs SET 10 mM (RP665) – To be discontinued

    dNTPs SET 10 mM is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates 10 mM of each dNTP (separately: dATP, dCTP, dGTP, dTTP), supplied as lithium salts solutions. (much higher stability) Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.
    RP665

    dNTPs MIX 8 mM (RP61) – Discontinued

    Ready-to-use dNTPs MIX 8 mM Total is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates (2 mM dATP, 2 mM dCTP, 2 mM dGTP, 2 mM dTTP), supplied as lithium salts solutions. (much higher stability) Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.
    RP61

    EXTRACTME GENOMIC DNA 96-WELL KIT (EM33) – Discontinued

    The EXTRACTME GENOMIC DNA 96-WELL KIT is designed for a rapid and efficient purification of high quality genomic, mitochondrial, bacterial, parasite or viral DNA from solid tissues , physiological fluids , fresh and frozen blood, human and animal mucosa membrane swabs , semen, hair, rodent tails, insects, bacteria, yeast and cell cultures.  The product is intended for research use only.
    EM33, EM33-192, EM33-960